A5016003147- Wheat and Barley Genetics and Pathology
- Chromosomal and Genetic Variations
- Genetic and Environmental Crop Studies
- Agricultural pest management studies
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Botanical Research and Chemistry
Carlsberg Laboratory
2021-2022
Improved agricultural and industrial production organisms are required to meet the future global food demands minimize effects of climate change. A new resource for crop microbe improvement, designated FIND-IT (Fast Identification Nucleotide variants by droplet DigITal PCR), provides ultrafast identification isolation predetermined, targeted genetic in a screening cycle less than 10 days. Using large-scale sample pooling combination with digital PCR (ddPCR) greatly increases size...
Lupins are high-protein crops that rapidly gaining interest as hardy alternatives to soybean; however, they accumulate antinutritional alkaloids of the quinolizidine type (QAs). Lupin domestication was enabled by discovery genetic loci conferring low QA levels (sweetness), but precise identity underlying genes remains uncertain. We show pauper , most common sweet locus in white lupin, encodes an acetyltransferase (AT) unexpectedly involved early pathway. In plants, a single-nucleotide...
Novel crop improvement methodologies, including the exploitation of natural genetic variation, are urgently required to feed our rapidly growing human population in context global climate change. Here we describe a ‘Fast Identification Nucleotide variants by DigITal PCR’ (FIND-IT) method for rapid identification pre-targeted or rare alleles large genomic populations. Libraries 500,000 individuals can be screened and desired isolated within two weeks. FIND-IT is widely applicable mining...