The recent outbreak of the novel coronavirus disease 2019 (COVID-19) has been labelled as a pandemic by World Health Organization. Although person-to-person transmission etiologic agent, severe acute respiratory syndrome 2 (SARS-CoV-2), confirmed, it is not known whether COVID-19 may be transmitted blood transfusion. Notwithstanding urgent requirement blood, critical to know SARS-CoV-2 virus can transfusion because many individuals asymptomatic carriers and donate blood. Several cases in...

We investigated the possible involvement of superoxide (.O2-) radical in alterations vascular reactivity and phosphoinositide (PI) turnover aortas from streptozotocin (STZ)-induced diabetic (4 week) rats. STZ treatment increased maximal contractile response aorta to norepinephrine (NE), phenylephrine (PE) high K+, whereas sensitivity remained unaltered. Ca(++)-induced contractions presence maximally effective concentrations PE K+ were also augmented after treatment. The was associated with...

Recent reports indicate that antibodies associated with heparin-induced thrombocytopenia and thrombosis (HITP) are specific for complexes formed between heparin the heparin-binding, platelet alpha granule protein, factor 4 (PF4). As other disorders mediated by immune (IC), characteristics of involved immunoglobulins could affect ability IC to cause symptoms. We therefore studied class, subclass, potency heparin:PF4 two groups patients: one severe thrombocytopenia, or without thrombosis, a...

Summary The role of repopulating lymphocytes after allogeneic stem cell transplantation (SCT) includes the prevention serious infections and attacking residual tumour cells in early post‐transplant phase. Therefore, current study analysed absolute lymphocyte count (ALC) on day 21 SCT predicting transplant outcomes 82 patients terms risk opportunistic recurrence original disease. median dose CD34 + , CD3 mononuclear (MNC) infused was 6·41 × 10 6 /kg, 1·96 8 /kg 6·81 respectively. high ALC...

Abstract: Objective: The therapeutic options currently available for treating refractory graft‐vs.‐host disease (GVHD) are limited. Therefore, the present study evaluated efficacy of mycophenolate mofetil (MMF) as a salvage treatment acute and chronic GVHD in allograft patients. Methods: Twenty‐six consecutive patients with (13 patients) or were enrolled. first‐line all consisted combination cyclosporine A (CyA) steroids, while was steroids without CyA according to risk group. MMF added at...

Abstract Multidrug resistance‐1 (MDR‐1) gene single nucleotide polymorphisms (SNPs) have been identified as associated with the treatment outcomes of acute myeloid leukemia (AML) in Caucasians; yet, similar evidence is lacking for Asian populations. A total 101 AML patients were enrolled current study. Two MDR1 SNPs (C3435T and G2677T/A) analyzed PCR/RFLP assay. As regards C3435T polymorphism, C/C genotype was significantly correlated lower functional P‐glycoprotein (P‐gp) activity leukemic...

In the analysis of red blood cell (RBC) aggregation using optical detection, various shearing methods have been used to disperse RBCs in confined geometries. This study investigated RBC measurement a microchip-stirring system by analys

The present study evaluated the serum levels of known angiogenic factors and analysed their prognostic significance in patients with acute or chronic leukemia. Enzyme-linked immunosorbent assays (ELISAs) were performed to quantify basic fibroblast growth factor (bFGF), vascular endothelial (VEGF), hepatocyte (HGF), tumor necrosis factor-alpha (TNF-alpha), angiogenin, matrix metalloproteinase-9 (MMP-9) stored samples obtained before treatment from myeloid leukemia (AML; 30 patients),...

Abstract Background Hereditary spherocytosis (HS) is the most common cause of inherited chronic hemolysis. The various tests developed for HS screening have many shortcomings. purpose this study was to develop a new, simple, reliable test using flow cytometry. Methods In new test, deionized water, hemolysis‐inducing agent, spiked red cell suspension during acquisition, and count cells measured sequentially in real‐time study, optimal parameters were established, discriminatory power adopted...

The bacterium Chlamydia trachomatis is one of the leading causes sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. infections, we evaluated a novel point-of-care (POC) test, aQcare TRF kit, which uses europium-chelated nanoparticles time-resolved fluorescence reader.The test performance was by comparing results obtained using POC testing kit with those nucleic acid amplification (NAAT), 114 NAAT-positive 327 NAAT-negative samples.The cut-off...

Abstract Background Deformability of red blood cells (RBCs) is a determinant flow resistance as RBCs pass through small capillaries the microcirculation. Available techniques for measuring RBC deformability often require washing process after each measurement, which not optimal day‐to‐day clinical use. Methods A laser diffraction technique has been combined with slit‐flow rheometry, shows significant advances in ektacytometric design, operation, and data analysis. The essential features this...

Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional indices are dimensionless values and not available for comparison across Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress aggregate RBCs under the shearing hydrodynamic force. The present study investigated relationships between by comparing measured with threshold using LORCA aggregometer. results showed did vary...

Purpose: We report our initial experience with transurethral injection of autologous adipose-derived regenerative cells (ADRCs) for the treatment urinary incontinence after radical prostatectomy.Materials and Methods: After providing written informed consent, six men persistent prostatectomy were enrolled in study.Under general anesthesia, about 50 mL adipose tissue was obtained from patients by liposuction.ADRCs separation centrifugation using Celution cell-processing device.A mixture ADRCs...

Abstract: Background : Little is known about the role of CD56+ natural killer (NK) cell dose on outcome allogeneic peripheral blood stem transplantation (PBSCT). Recently, higher NK cells has been associated with a lower incidence severe graft‐versus‐host disease (GVHD). The current study attempted to evaluate effect transplant outcomes in PBSCT setting. Methods and materials Sixty‐one cytokine mobilized PBSC recipients were analyzed according infused CD34+ relation overall survival (OS),...

The current study attempted to evaluate the association between IL-10 promoter gene polymorphism and transplant outcomes including occurrence of chronic graft-versus-host disease (GVHD) its clinical course during systemic immunosuppressive treatment (IST) among 60 recipients cytokine-mobilized peripheral blood stem cell (PBSC) from HLA-matched sibling donors.We analyzed 3 single-nucleotide polymorphisms in proximal region (-1082/-819/-592).In study, only two haplotypes (1082*A/819*T/592*A...

The work was done to study immunogenetic peculiarities of neuroinflammatory diseases among Korean children. A total 13 children with (8 males and 5 females; mean age 4.6 +/-2.6 yr) were consecutively recruited. Geno-mic typing performed on their HLA DRB/HLA DQB genes using PCR-SSOP/SSP techniques gel immunoelectrophoresis. frequencies HLA-DR1 *15 in acute disseminated encephalomyelitis (ADEM) (31%) DQB1 *06 other (38%) significantly increased compared control subjects. HLA-DRB3 * 0202...

The effect of the transplant dose each cell subset on engraftment kinetics and transplantation outcomes was evaluated in HLA-identical allogeneic peripheral blood stem (PBSCT). Sixty-nine patients were included this retrospective study. Engraftment kinetics, outcomes, immune reconstitution up to 1 year after analyzed according CD34+ non-CD34+ cells, including natural killer (NK) cells CD8+ cytotoxic T (Tc) cells. An accelerated neutrophil strongly associated with a higher NK (12 versus 16...