A5101397706- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant responses to water stress
- Gene expression and cancer classification
- Plant Gene Expression Analysis
- Sexual Differentiation and Disorders
- Bacteriophages and microbial interactions
- Molecular Biology Techniques and Applications
- Plant biochemistry and biosynthesis
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Connective tissue disorders research
- Phytochemicals and Antioxidant Activities
- melanin and skin pigmentation
- Hearing, Cochlea, Tinnitus, Genetics
- Cancer Research and Treatments
- Prenatal Screening and Diagnostics
- Medicinal Plant Pharmacodynamics Research
- Renal and related cancers
- Phytochemistry and Biological Activities
- Genetic and Kidney Cyst Diseases
- Immunodeficiency and Autoimmune Disorders
First Affiliated Hospital of Zhengzhou University
2012-2022
Cangzhou Central Hospital
2021
Chengdu University of Traditional Chinese Medicine
2020-2021
Guangxi Medicinal Botanical Garden
2007-2021
Zhengzhou University
2012
Southeast University
2011
First Affiliated Hospital of Jiangxi Medical College
2006
Nanfang Hospital
2005
Southern Medical University
2002-2003
Safflower is widely used in dying and traditional medicine, C-glucosylquinochalcones are the main metabolic species red color of safflower. Various safflower cultivars have flowers with different colors. However, transcriptional differences among different-colored genes participating C-glucosylquinochalcone biosynthesis largely unknown. To provide insights on this issue, we performed integrated metabolomics transcriptome analyses flavonoid colors (white-W, yellow-Y, light red-LR, deep...
MicroRNAs (miRNAs) are small, non-coding RNAs that important regulators of gene expression, and play major roles in plant development their response to the environment. Root extracts from Panax notoginseng contain triterpene saponins as principal bioactive constituent, demonstrate medicinal properties. To investigate novel conserved miRNAs P. notoginseng, three small RNA libraries constructed 1-, 2-, 3-year-old roots which root saponin levels vary underwent high-throughput sequencing. roots,...
Abstract Spatholobus suberectus Dunn ( S . ), which belongs to the Leguminosae, is an important medicinal plant in China. Owing its long growth cycle and increased use human medicine, wild resources of have decreased rapidly may be on verge extinction. De novo assembly whole genome provides us a critical potential resource towards biosynthesis main bioactive components seed development regulation mechanism this plant. Utilizing several sequencing technologies such as Illumina HiSeq X Ten,...
Background Safflower ( Carthamus tinctorius L.) is a domesticated species with long history of cultivation and widespread distribution across the globe, light plays an important role in controlling its boundary. Flowers from safflower have been widely used traditional Chinese medicine because their ability to improve cerebral blood flow. Flavonoids are main active compounds many pharmacological effects. In this study, we aimed explore relationship between different intensities flavonoid...
45,X/46,XY mosaicism is a rare chromosomal abnormality and probably underdiagnosed. Although clinical genetic analyses have been performed in some disorders of sexual development, there few studies focusing on the phenotype details mosaicism, especially Chinese population. The aim this study was to describe experience our service relation 16 cases with mosaicism. age at first evaluation patients ranged from 43 days 30 years. Eight were reared as female 8 male. main reasons for examination...
Interferon regulatory factor (IRF) 2 is a transcription belonging to the IRF family, which essential for gasdermin D (GSDMD)‑induced pyroptosis. Decreasing myocardial cell pyroptosis confers protection against heart damage and cardiac dysfunction caused by infarction (MI). The aim of present study was investigate involvement IRF2 in MI underlying mechanism To mimic MI, ligation left anterior descending coronary artery performed establish an vivo mouse model rat cardiomyocytes H9c2 cells were...
Flowers of safflower (Carthamus tinctorius L.) have been widely used in traditional Chinese medicine because their ability to improve cerebral blood flow. The major active ingredients are flavonoids. To date, few flavonoid biosynthesis genes cloned from this species. In our previous research, four isoform chalcone isomerase were screened. study, a new gene (designated as CtCHI-N) was cDNA library. A 696-bp for CtCHI-N cloned, and its sequence subsequently analyzed. recombinant protein...
Pharmacognosy Magazine ,2019,15,60,18-23.DOI:10.4103/pm.pm_317_18Published:January 2019Type:Original Article Authors:Xiaoying Chen, Shuangshuang Qin, Cui Li, Qinghua Wu, Chao Jiang, Jian Yang, and Yang Author(s) affiliations:Xiaoying Chen1, Qin1, Li1, Wu1, Jiang2, Yang2, Xiaoyun Guo1, Chunli Ou1 1Guangxi Key Laboratory of Medicinal Resources Protection Genetic Improvement, Guangxi Botanical Garden Plants, Nanning, Guangxi, China 2National Resource Center for Chinese Materia Medica, Academy...
Abstract Background Waardenburg syndrome (WS) is a dominantly inherited, genetically heterogeneous auditory‐pigmentary characterized by nonprogressive sensorineural hearing loss and iris discoloration. This study aimed to investigate the underlying molecular pathology in Chinese WS families. Methods A total of 13 patients with type II (WS2) from six unrelated families were enrolled. We investigated mutation profile genes related congenital deafness these through targeted sequencing...
Lonicera hypoglauca Miq, which is widely distribute in south China, an important Chinese plant used traditional medicine. Here we report the first complete chloroplast (cp) genome sequence of this species. The circular cp 154,581 bp size, including a large single-copy (LSC) region 88,379 and small (SSC) 18,646 bp, were separated by two inverted repeat (IR) regions (IRA B, 23,778 each). A total 121 genes annotated, 8 ribosomal RNAs (rRNAs), 33 transfer (tRNAs) 80 protein-coding (PCGs)....
To perform mutation screening and prenatal diagnosis for a five-generation Chinese pedigree with autosomal dominant congenital nuclear cataract from Henan province by DNA sequencing.Blood samples were taken the family members. Four candidate genes (CRYBA1/A3, CRYBB1, CRYBB2 CRYGD) screened mutations using direct sequencing. Prenatal genetic was provided fetus at early gestation through chorionic villus sampling.A missense mutation, c.387C to A, detected in exon 4 of CRYBB1 gene all patients....
To explore the genetic etiology of three pedigrees with a gestational history fetal renal anomalies.Peripheral venous blood or skin samples were derived from probands pedigrees. Copy number variation sequencing (CNV-seq) was applied to detect alterations genome CNVs.The patient pedigree 1 and fetuses 2 3 all carried heterozygous 17q12 deletion, size ranging 1.4 Mb 1.48 encompassing HNF1B gene.The diagnosis microdeletion may be difficult during period for its variable phenotypes. Alterations...
The complete chloroplast genome of Lonicera similis Hemsl. has been characterized by reference-based assembly using Illumina paired-end data. circular cp is 155,463 bp in length, containing a large single copy (LSC) region 89,282 bp, small (SSC) 18,661 which are separated pair inverted repeat (IR) regions 23,760 bp. A total 129 genes were predicted from the genome, including 83 protein-coding genes, 37 tRNA and eight rRNA genes. Phylogenetic analysis reveals that L. more closely related to...
Abstract Nephrolithiasis is not common in children, but the incidence gradually increased these years. Urinary tract malformations, urinary infection, dietary habits, geographic region and genetic factor are involved etiology of nephrolithiasis. For affected child, it especially important to elucidate etiology, which may provide an accurate diagnosis, a personalized therapy effective follow-up strategy. Here seek ten-year-old boy incidentally found with nephrolithiasis, next generation...
To identify potential mutations of ED1 gene in six pedigrees with hypohidrotic ectodermal dysplasia (HED), and to provide genetic counseling prenatal diagnosis.Eight coding exons patients clinically diagnosed HED their relatives were amplified by polymerase chain reaction (PCR). The products further analyzed direct sequencing.Various detected, which included R153C, A349T, G299S, A349T X392Q. Heterozygous double peaks at the same position found female carriers. Deletion exon 9 was detected...
The phenotype and genetics of three patients with autosomal recessive polycystic kidney disease (ARPKD) at childhood, teenage advanced age were analyzed.Next generation sequencing (NGS) was applied to all the probands. PCR Sanger used verify suspicious gene variants screened by NGS in probands their family members, one got prenatal diagnosis.Through NGS, sequencing, 5-yr proband pedigree 1 shown carry compound heterozygous c.5935G>A(p.G1979R) c.5428G>T(p.E1810X) PKHD1, originated from his...
Objective: To detect gene mutation sassociated with deafness in four Waardenburg syndrome (WS) type Ⅱ patients, and to explore the possible mechanism of molecular genetics. Methods: All patients WS were identified at genetic prenatal diagnosis center First Affiliated Hospital Zhengzhou University from August 2015 December 2018.Clinical materials peripheral blood collected family members. The genes associated tested by next generation sequencing(NGS). And suspected mutations verified Sanger...