A5038300775- Neuroscience and Neuropharmacology Research
- CRISPR and Genetic Engineering
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Cholinesterase and Neurodegenerative Diseases
- Advanced biosensing and bioanalysis techniques
- Acute Myeloid Leukemia Research
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- HIV Research and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- Parkinson's Disease Mechanisms and Treatments
- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Amyotrophic Lateral Sclerosis Research
- Histone Deacetylase Inhibitors Research
- Connexins and lens biology
- Animal Genetics and Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Insect Resistance and Genetics
- Biochemical and Molecular Research
- Molecular Biology Techniques and Applications
- Health and Medical Education
- Diabetes and associated disorders
The University of Sydney
2024-2025
Universidad de la República
1998-2024
Collège de France
1992-1997
Inserm
1992-1997
The University of Texas MD Anderson Cancer Center
1993
Instituto de Investigaciones Biológicas Clemente Estable
1988-1991
Universidad de Montevideo
1990
The University of Texas System
1978-1983
University of Houston
1983
Anderson Hospital
1978
Anandamide is an endogenous ligand for central cannabinoid receptors and released after neuronal depolarization. increased protein tyrosine phosphorylation in rat hippocampal slices neurons culture. The action of anandamide resulted from the inhibition adenylyl cyclase cyclic adenosine 3′,5′-monophosphate-dependent kinase. One proteins phosphorylated response to was isoform pp125-focal adhesion kinase (FAK+) expressed preferentially neurons. Focal a involved interactions between integrins...
The mechanisms by which stimuli that raise cytosolic free Ca2+ concentrations in neurons can increase protein tyrosine phosphorylation are not known. Using rat hippocampal slices and cortical synaptosomes, we have examined the regulation of two highly related cytoplasmic kinases, pp125 focal adhesion kinase (pp125(FAK)) proline-rich 2/cell beta (PYK2/CAKbeta). Membrane depolarization increased PYK2/CAKbeta pp125(FAK). These effects were blocked EGTA or C inhibitors (RO31-8220; GF109203X)...
Abstract Atypical wild-type superoxide dismutase 1 (SOD1) protein misfolding and deposition occurs specifically within the degenerating substantia nigra pars compacta (SNc) in Parkinson disease. Mechanisms driving formation of this pathology relationship with SNc dopamine neuron health are yet to be fully understood. We applied proteomic mass spectrometry synchrotron-based biometal quantification post-mortem brain tissues from disease patients age-matched controls uncover key factors...
Although protein phosphatases appear to be highly controlled in intact cells, relatively little is known about the physiological regulation of their activity. DARPP-32, a dopamine- and cAMP-regulated phosphoprotein apparent M(r) 32,000, phosphorylated vitro by casein kinase I, II, cAMP-dependent on sites vivo. DARPP-32 Thr-34 potent inhibitor phosphatase 1 an excellent substrate for calcineurin, Ca2+/calmodulin-dependent phosphatase. Here we provide evidence, using both purified proteins...
During neuropathological states associated with inflammation, the levels of cytokines such as interleukin-1beta (IL-1beta) are increased. Several studies have suggested that neuronal damage observed in pathogenesis implicating IL-1beta caused by an alteration neurochemical interactions between neurons and astrocytes. We report here treating striatal astrocytes primary culture for 22-24 hr enhances ATP-evoked release arachidonic acid (AA) no effect on ATP-induced accumulation inositol...
Abstract FAK+, an isoform of focal adhesion kinase preferentially expressed in brain and PYK2/Cakβ (proline‐rich tyrosine 2/cell kinaseβ) are two related cytoplasmic kinases. They candidates for coupling electrical activity stimulation neurotransmitter receptors to short long‐term changes synaptic properties, cytoskeletal organization gene expression neurons. As the same set stimuli appear capable stimulating FAK and/or PYK2 non‐neuronal cells cell lines with neuronal characteristics, we...
Abstract: In rat hippocampal slices and in neurons primary culture, K + ‐induced depolarization increased markedly rapidly tyrosine phosphorylation of a 110‐kDa protein (pp110) and, to lesser degree, 120‐kDa (pp120), calcium‐dependent fashion. Qlutamate, 1‐aminocyclopentane‐ trans ‐1,3‐dicarboxylic acid (an agonist metabotropic glutamate receptors), α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic ionotropic receptors) stimulated also pp110 pp120. These effects were not observed astrocytes...
Early in HIV infection, CD4+ lymphocytes exhibit the properties of an anergic state characterized by unresponslveness to mltogens or TCR stimulation and defective IL-2 production. As tyrosine phosphorylation is earliest biochemical events initiated CD3-TCR, we studied protein purified from 25 asymptomatic seropositlve patients (CD4 T cells >350/mm3) previously stimulated vitro immobilized antl-CD3 mAb co-lmmoblllzed antl-CD28 mAbs. Purified HIV-lnfected exhibited early response CD3...
Electrophoretic shift mutants induced in Chinese hamster ovary (CHO) cells indicate that these not extensively functionally hemizygotic. Therefore, effective haploidy is unsatisfactory as a general theory to explain the frequency of recessive this cell line. CHO were screened for electrophoretic variants enzymes coded by approximately 40 genetic loci. Clones isolated after exposure ultraviolet radiation examined starch gel and Cellogel electrophoresis. Shift recovered representing 11...
Concordant segregation analysis of Chinese hamster <i>(Cricetulus griseus</i>) isozymes and chromosomes segregating from interspecific somatic cell hybrids made with mouse C11D cells revealed the locations <i>GPI </i>and <i>PEPD </i>on chromosome 9 <i>TPI 8 in both euploid CHO cells. The patterns electrophoretically detectable shift mutants these loci were consistent observed presence two normally banded 8’s monosomy for 9. These findings isolation...
Even though the short-term actions of dopamine on postsynaptic receptors are well-characterized, molecular bases for long-term trophic interactions between neurons and their targets remain unclear. Since protein-tyrosine phosphorylation plays a key role in action factors, we have investigated its possible involvement striatal targets. Lesioning rat nigrostriatal by using 6-hydroxydopamine increased tyrosine several proteins, including major 180-kDa protein (pp180) ipsilateral striatum....
Mitomycin C (MMC)-resistant interspecific somatic cell hybrids made between human cells and the MMC-sensitive, Chinese hamster ovary (CHO) excision repair-deficient UV41 generally contained chromosome 16, while other chromosomes were randomly present. MMC-sensitive -resistant subclones isolated from resistant clones resistance segregated concordantly with 16 markers. UV radiation survival analysis of indicated that MMC correlated. Therefore complementing gene, Excision Repair Cross...
Polyethylene glycol-mediated fusion of Chinese hamster ovary (CHO) cells with mouse Cl1D produced interspecific somatic cell hybrids which slowly segregated CHO chromosomes. Cytogenetic and isozyme analysis HAT- bromodeoxyuridine-selected hybrid subclones members a clone panel retaining different combinations chromosomes enabled provisional assignments the following enzyme loci to chromosomes: TK, GALK, ACP1 chromosome 7; TK GALK Z13; ACP1, ADA, ITPA Z8; ADA Z9. These genetic markers reflect...
Introducción: La pandemia impactó en la calidad de vida, salud integral y formación los estudiantes posgrados a nivel mundial. Objetivos: Describir percepción del efecto académica posgrado pediatría Uruguay durante años 2019- 2021. Metodología: Estudio descriptivo, transversal mediante encuesta las generaciones 2019, 20 21. Se analizaron variables sociodemográficas salud, rendimiento.Resultados: Participaron 93 (65% habilitados). mediana edad fue 30,5 años, género femenino: 88%. Cursaron...
Abstract Atypical wild-type superoxide dismutase 1 (SOD1) protein misfolding and deposition occurs specifically within the degenerating substantia nigra pars compacta (SNc) in Parkinson disease. Mechanisms driving formation of this pathology relationship with SNc dopamine neuron health, are yet to be fully understood. We applied proteomic mass spectrometry synchrotron-based biometal quantification post-mortem brain tissues from disease patients age-matched controls uncover key factors...
En este trabajo se presentan los resultados de una indagacion caracter diagnostico acerca del desempeno academico estudiantes Medicina en cursos y examenes correspondientes al ciclo Basico Clinico Comunitario (semestres 2 6 la carrera) el lapso 2009 a 2016. Los datos inscripciones, aprobaciones no obtuvieron sistematizaron partir fuentes secundarias oficiales origen institucional. Se realizo un analisis cuantitativo ex-post en estudio tipo descriptivo que incluyo estadistico inferencial uno...