A5070311875- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinoids in leukemia and cellular processes
- Garlic and Onion Studies
- Nanoparticle-Based Drug Delivery
- Advanced Drug Delivery Systems
- Listeria monocytogenes in Food Safety
- Respiratory viral infections research
- Soil and Unsaturated Flow
- Neonatal Respiratory Health Research
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Heparin-Induced Thrombocytopenia and Thrombosis
- Nephrotoxicity and Medicinal Plants
- Advancements in Transdermal Drug Delivery
- Drug-Induced Adverse Reactions
- IL-33, ST2, and ILC Pathways
- Clay minerals and soil interactions
- Viral gastroenteritis research and epidemiology
University of Michigan–Ann Arbor
2016-2024
Aligarh Muslim University
2009-2023
Michigan United
2018
Acute exacerbations are the major cause of morbidity and mortality in patients with chronic obstructive pulmonary disease (COPD). Rhinovirus, which causes acute may also accelerate progression lung these patients. Current therapies reduces respiratory symptoms does not treat root effectively. We hypothesized that quercetin, a potent antioxidant anti-inflammatory agent antiviral properties be useful treating rhinovirus-induced changes COPD. Mice COPD phenotype maintained on control or...
Abstract Airway epithelial cells are the major target for rhinovirus (RV) infection and express proinflammatory chemokines antiviral cytokines that play a role in innate immunity. Previously, we demonstrated RV interaction with TLR2 causes ILR-associated kinase-1 (IRAK-1) depletion both airway macrophages. Further, IRAK-1 degradation caused by activation was shown to inhibit ssRNA-induced IFN expression dendritic cells. Therefore, this study, examined of RV-induced IFN-β, IFN-λ1, CXCL-10,...
We have demonstrated that intranasal immunotherapy with allergens formulated in a nanoemulsion (NE) mucosal adjuvant suppresses Th2/IgE-mediated allergic responses and protects from allergen challenge murine food allergy models. Protection conferred by this therapy is associated strong suppression of specific Th2 cellular immunity increased Th1 cytokines. Here we extend these studies to examine the effect NE-allergen immunization mice sensitized multiple foods. Mice were both egg peanut then...
Current COVID-19 mRNA vaccines delivered intramuscularly (IM) induce effective systemic immunity, but with suboptimal immunity at mucosal sites, limiting their ability to impart sterilizing immunity. There is strong interest in rerouting immune responses induced the periphery by parenteral vaccination portal entry site of respiratory viruses, such as SARS-CoV-2, vaccination. We previously demonstrated combination adjuvant, NE/IVT, consisting a nanoemulsion (NE) and an RNA-based RIG-I agonist...
Retinoic acid (RA), controls the immunoregulatory functions of many immune cells, including dendritic cells (DCs), and is important for mucosal immunity. In DCs, RA regulates expression pattern recognition receptors stimulates interferon production. Here, we investigated role in DCs mounting immunity to respiratory syncytial virus (RSV). To abolish signaling used mice expressing a dominant negative form retinoic receptor-α (RARα) under CD11c promoter (CD11c-dnRARα). Paradoxically, upon RSV...
Therapeutics, based on small interfering RNA (siRNA), have demonstrated tremendous potential for treating cancer. However, issues such as non-specific targeting, premature degradation, and the intrinsic toxicity of siRNA, to be solved before they are ready use in translational medicines. To address these challenges, nanotechnology-based tools might help shield siRNA ensure its specific delivery target site. Besides playing a crucial role prostaglandin synthesis, cyclo-oxygenase-2 (COX-2)...
The objective of the study was to explore feasibility a new drug delivery system using laponite (LAP) and cyclic poly(ethylene glycol) (cPEG). Variously shaped flexible hybrid nanocrystals were made by both covalent physical attachment chemically homogeneous cyclized PEG nanodisc plates. size resulting, nearly spherical particles ranged from 1 1.5 µm, while PEGylation with linear methoxy poly (ethylene (mPEG) resulted in fragile sheets different shapes sizes. When infused 10% doxorubicin...
The present study deals with the evaluation of efficacy amoxicillin bearing poly-lactic-glycolic acid (PLGA) microsphere formulation in treatment experimental listeriosis Swiss albino mice. Amoxicillin PLGA microspheres were prepared by water-in-oil-in-water emulsion technique. microwspheres significantly regulated sustained release encapsulated drug over extended time period. rate increased temperature dependent manner. successfully cleared bacterial burdens vital organs (kidney, spleen,...
In the present study, we evaluated potential of a liposomal formulation garlic oil component DAS (diallyl sulfide) in treating disseminated infection caused by intracellular opportunistic pathogen Candida albicans experimental mice. The PC (phosphatidylcholine) was for size, zeta-potential, entrapment efficiency and release kinetics, toxicity etc. For therapeutic studies, mice were challenged with intravenous dosage 10(7) blastospores C. followed treatment various doses formulations [12 6...
The present study was aimed at evaluating the effectiveness of amoxicillin-bearing HSA (human serum albumin) and PLGA [poly(lactic-co-glycolic acid)] microparticles in combating Listeria monocytogenes infection Swiss albino mice. Amoxicillin-bearing microspheres were prepared by chemical cross-linking a drug/albumin mixture with glutaraldehyde, W/O/W (water-in-oil-in-water) emulsion technique. characterized for their size, ζ potential entrapment efficiency using SEM (scanning electron...
Mucosal surfaces are the primary point of entry for many infectious agents and mucosal immune responses serve as defense to these pathogens. In order mount an effective response, it is important induce T cell homing surfaces. Conventional vaccine adjuvants strong systemic immunity but often fail produce immunity. We have developed oil-in-water nanoemulsion (NE) adjuvant that provides efficient protection against pathogens when administered part intranasal vaccine. present study, we...
Abstract Vitamin A and its biologically active metabolites, all-trans 9-cis retinoic acid (RA), are thought to be important in generating modulating immune function. However, RA modulates the function of many types cells, specific role dendritic cell (DC) activation, Ag presentation, T effector has not been fully characterized. Because works primarily through receptor (RAR)α, we examined mice with a myeloid cell–specific defect signaling. These transgenic have CD11c-cre–driven expression...
Abstract Mucosal surfaces are the primary point of entry for most infectious agents. Therefore, induction mucosal immune responses serves as defense by preventing and clearing pathogens. In order to mount an effective response, secretory IgA T cell homing towards two very important factors. Conventional vaccines induce strong systemic immunity, but often fail produce responses. Our group has developed oil-in-water nanoemulsion which been shown provide protection against pathogens when...
Designing an adjuvant that induces mucosal as well systemic immunity has been long desired. Mucosal vaccines are more effective in combating respiratory pathogens they induce specific IgA and T cell responses. However, developing adjuvants is very challenging due to nature of the barrier environment. In our previous studies we have shown intranasal (oil-in-water based nanoemulsion-NE) interacts with epithelial cells dendritic cell-specific retinaldehyde dehydrogenase (RALDH) activity...