We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at biologic pH 7.4. Conversion into dendrimer was achieved by irradiating solutions with UV light reduce bound cations...

Hepatitis B virus infection remains an important global health concern despite the availability of safe and effective prophylactic vaccines. Limitations to these vaccines include requirement for refrigeration three immunizations thereby restricting use in developing world. A new nasal hepatitis vaccine composed recombinant surface antigen (HBsAg) a novel nanoemulsion (NE) adjuvant (HBsAg-NE) could be with fewer administrations.

Abstract Edible films based on potato starch, chitosan and Aloe vera gel (AV) as modifiers were evaluated towards their potential application in food or cosmetics industry. The films, with different AV concentration prepared by casting method. influence of UV radiation samples chemical structure was analyzed. surface morphology observed AFM molecular arrangement mixture components analyzed ATR–FTIR spectroscopy X-ray diffraction. Hydrogen bond interactions among the film components,...

ABSTRACT The currently available commercial human anthrax vaccine requires multiple injections for efficacy and has side effects due to its alum adjuvant. These factors limit utility when immunizing exposed populations in emergent situations. We evaluated a novel mucosal adjuvant that consists of nontoxic, water-in-oil nanoemulsion (NE). This material does not contain proinflammatory component but penetrates surfaces load antigens into dendritic cells. Mice guinea pigs were intranasally...

Epidemiological and experimental data suggest that both robust neutralizing antibodies potent cellular responses play important roles in controlling primary HIV-1 infection. In this study we have investigated the induction of systemic mucosal immune to HIV gp120 monomer immunogen administered intranasally a novel, oil-in-water nanoemulsion (NE) adjuvant. Mice guinea pigs immunized by application recombinant antigen mixed NE demonstrated serum anti-gp120 IgG, as well bronchial, vaginal, IgA...

Abstract Nasal administration of an oil-in-water nanoemulsion (NE) adjuvant W805EC produces potent systemic and mucosal, Th-1– Th-17–balanced cellular responses. However, its molecular mechanism action has not been fully characterized is particular interest because NE does contain specific ligands for innate immune receptors. In these studies, we demonstrate that activates immunity, induces gene transcription, modulates NF-κB activity via TLR2 TLR4 by a appears to be distinct from typical...

Several SARS-CoV-2 vaccines have received EUAs, but many issues remain unresolved, including duration of conferred immunity and breadth cross-protection. Adjuvants that enhance shape adaptive immune responses confer broad protection against variants will be pivotal for long-term as drift continue to emerge. We developed an intranasal, rationally designed adjuvant integrating a nanoemulsion (NE) activates TLRs NLRP3 with RNA agonist RIG-I (IVT DI). The combination spike protein antigen...

Aim: Epicutaneous immunotherapy (EPIT) with peanut has been demonstrated to be safe but efficacy may limited by allergen uptake through the skin barrier. To enhance into skin, authors used peanut-coated microneedles and compared them EPIT in a allergy mouse model. Methods: Sensitized mice were treated or peanut-EPIT then challenged determine protection. Results: Treatment was showed enhanced desensitization administered via similar schedule. Protection associated reduced Th2 immune responses...

The present article focuses on the characterization of new biocomposites poly(butylene succinate) (PBS) with fillers plant origin such as onion peels (OP) and durum wheat bran WB (Triricum durum) subjected to composting artificial aging. susceptibility fungal growth, cytotoxicity antibacterial properties were also examined. biodegradation samples was investigated under normalized conditions simulating an intensive aerobic process. It shown that tested natural significantly accelerate process...

The effect of the new bioproduct in form granulate and liquid concentrate on PLA biodegradation during industrial composting was investigated. analysis included compost parameters, mass loss, structural changes (SEM) chemical properties (FTIR-ATR, O/C ratio). effectiveness bioproduct, especially form, accelerating degradation improvingcompost confirmed what matters for sustainable waste management.

While the nasal mucosa is a potentially useful site for human immunization, toxin-based adjuvants are generally unsafe and less effective in humans. Safe mucosal that activate protective immunity via administration highly dependent on barrier antigen sampling by epithelial DCs. Here, we demonstrate protein antigens formulated unique oil-in-water nanoemulsions (NEs) result distinctive transcellular uptake ciliated cells, leading to delivery into associated lymphoid tissue. NE formulation also...

Vaccine adjuvants have been reported to induce both mucosal and systemic immunity when applied surfaces this dual response appears important for protection against certain pathogens. Despite the potential advantages, however, no are currently approved human use. Evaluating compounds as is a slow costly process due need lengthy animal immunogenicity studies. We constructed library of 112 intranasal adjuvant candidate formulations consisting oil-in-water nanoemulsions that contain various...

Food allergy is a growing health concern worldwide. Current allergen-specific immunotherapy (AIT) approaches require frequent dosing over extended periods of time and may induce anaphylaxis due to allergen-effector cell interactions. A critical need remains develop novel that refine AIT for the treatment food allergies. Previous studies show poly(lactide-co-glycolide) (PLG) nanoscale particles (NP) effectively suppress Th1- Th17-driven immune pathologies. However, their ability distinct...

The polymer processing industry is paying more attention to biodegradable materials synthesized from renewable sources. One of the most popular them polylactide (PLA). Except material which a given product made, particularly important process manufacturing material, processing, use by consumer, and finally, recycling it. Neither these steps indifferent environment. polymers can often lead degradation, affects properties leads generation substantial amounts post-production waste that cannot...

Live-virus vaccines for smallpox are effective but have risks that no longer acceptable routine use in populations at minimal risk of infection. We developed a mucosal, killed-vaccinia virus (VV) vaccine based on antimicrobial nanoemulsion (NE) soybean oil and detergent. Incubation VV with 10% NE least 60 min causes the complete disruption inactivation VV. Simple mixtures (Western Reserve serotype) (VV/NE) applied to nares mice resulted both systemic mucosal anti-VV immunity,...

TH2-biased immune responses are associated with inadequate protection against some pathogens and cancer, colitis, asthma allergy. Since most currently used vaccine adjuvants induce a response, this has led to interest in developing capable of activating TH1 immunity modulating existing TH2 responses. Immunotherapies shift from have generally required prolonged immunization protocols not induced effective We demonstrated that nanoscale emulsions (NE), novel mucosal adjuvant, robust IgA IgG...

Current influenza virus vaccines are focused on humoral immunity and limited by the short duration of protection, narrow cross-strain efficacy, suboptimal immunogenicity. Here, we combined two chemically biologically distinct adjuvants, an oil-in-water nanoemulsion (NE) RNA-based agonists RIG-I, to determine whether diverse mechanisms these adjuvants could lead improved immunogenicity breadth protection against virus. NE activates TLRs, stimulates immunogenic apoptosis, enhances cellular...