A5049959275- Malaria Research and Control
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Vector-borne infectious diseases
- Drug Transport and Resistance Mechanisms
- Healthcare and Venom Research
- Hemoglobinopathies and Related Disorders
- Aquaculture disease management and microbiota
- vaccines and immunoinformatics approaches
- Invertebrate Immune Response Mechanisms
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
Radboud University Medical Center
2024
University Hospital Heidelberg
2020-2024
Heidelberg University
2020-2024
Radboud University Nijmegen
2024
University of Lisbon
2016-2022
National Center for Infectious Diseases
2020
Multiplexed PCR amplicon sequencing (AmpSeq) is an increasingly popular application for cost-effective monitoring of threatened species and managed wildlife populations, shows strong potential the genomic epidemiology infectious disease. AmpSeq data from microbes can inform disease control in multiple ways, such as by measuring drug resistance marker prevalence, distinguishing imported local cases, determining effectiveness therapeutics. We describe design comparative evaluation two new...
Intracellular pathogens have evolved mechanisms to ensure their survival and development inside host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei uptake via GLUT1 transporter specifically enhanced in P. berghei-infected We further ATP levels cells containing developing parasites are decreased, which known enhance membrane activity. In addition, molecules translocated cell, increasing at later stages infection....
Abstract Following Plasmodium falciparum infection, individuals can remain asymptomatic, present with mild fever in uncomplicated malaria cases, or show one more severe symptoms. Several studies have investigated associations between parasite transcription and clinical severity, but no broad conclusions yet been drawn. Here, we apply a series of bioinformatic approaches based on P. ’s tightly regulated transcriptional pattern during its ~48-hour intraerythrocytic developmental cycle (IDC) to...
Persistence of malaria parasites in asymptomatic hosts is crucial areas seasonally-interrupted transmission, where P. falciparum bridges wet seasons months apart. During the dry season, infected erythrocytes exhibit extended circulation with reduced cytoadherence, increasing risk splenic clearance cells and hindering parasitaemia increase. However, what determines parasite persistence for long periods time remains unknown. Here, we investigated whether seasonality affects plasma composition...
Abstract Two malaria parasite species, Plasmodium falciparum ( Pf ) and P. vivax Pv are responsible for most of the disease burden caused by malaria. Vaccine development against this has focused mainly on . Whole-sporozoite (WSp) vaccination, targeting pre-erythrocytic (PE) stages, is a promising strategy immunization several WSp-based vaccine candidates currently undergoing clinical evaluation. In contrast, no WSp have been developed , due to constraints in production sporozoites...
Whole-sporozoite vaccination/immunization induces high levels of protective immunity in both rodent models malaria and humans. Recently, we generated a transgenic line the parasite P. berghei (Pb) that expresses falciparum (Pf) circumsporozoite protein (PfCS), showed this (PbVac) was capable (1) infecting developing human hepatocytes but not erythrocytes, (2) inducing neutralizing antibodies against Pf parasite. Here, analyzed PbVac detail developed tools necessary for its use clinical...
Abstract Multiplexed PCR amplicon sequencing (AmpSeq) is an increasingly popular application for cost-effective monitoring of threatened species and managed wildlife populations, shows strong potential genomic epidemiology infectious disease. AmpSeq data microbes can inform disease control in multiple ways, including measuring drug resistance marker prevalence, distinguishing imported from local cases, determining the effectiveness therapeutics. We describe design comparative evaluation two...