A5100402083- Retinal Development and Disorders
- Retinal Diseases and Treatments
- melanin and skin pigmentation
- Heme Oxygenase-1 and Carbon Monoxide
- Retinoids in leukemia and cellular processes
- Connexins and lens biology
- Glaucoma and retinal disorders
- Cancer-related molecular mechanisms research
- Ocular Disorders and Treatments
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- Mosquito-borne diseases and control
- Adenosine and Purinergic Signaling
- RNA modifications and cancer
- Corneal Surgery and Treatments
- Mast cells and histamine
- Ocular Oncology and Treatments
- RNA regulation and disease
- Cancer-related gene regulation
- Biosensors and Analytical Detection
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Ocular Surface and Contact Lens
Affiliated Eye Hospital of Wenzhou Medical College
2015-2025
Wenzhou Medical University
2015-2025
Zhangjiakou Academy of Agricultural Sciences
2024
First Affiliated Hospital of Wenzhou Medical University
2024
Shanxi Agricultural University
2024
Nanjing Medical University
2023
Wenzhou Hospital of Traditional Chinese Medicine
2022
Yueyang Hospital
2016-2022
Shanghai University of Traditional Chinese Medicine
2016-2022
Zhejiang Chinese Medical University
2022
Compromised clearance of all-trans-retinal (atRAL), a component the retinoid cycle, increases susceptibility mouse retina to acute light-induced photoreceptor degeneration. Abca4−/−Rdh8−/− mice featuring defective atRAL were used examine one or more underlying molecular mechanisms, because exposure intense light causes severe degeneration in these animals. Here we report that bright increased levels induced rapid NADPH oxidase-mediated overproduction intracellular reactive oxygen species...
Although several genetic and biochemical factors are associated with the pathogenesis of retinal degeneration, it has yet to be determined how these different impairments can cause similar degenerative phenotypes. Here, we report microglial/macrophage activation in both a Stargardt disease age-related macular degeneration mouse model caused by delayed clearance all-trans-retinal from retina, retinitis pigmentosa impaired pigment epithelium (RPE) phagocytosis. Mouse microglia displayed RPE...
Abstract Hypertonic stress activates T cells by inducing the controlled release of ATP through pannexin-1 hemichannels and activation ATP-gated ion channels P2X1, P2X4, P2X7. saline (HS) resuscitation increases cell function inhibits posttraumatic anergy, which can reduce immunosuppression sepsis in trauma patients. We have previously shown that HS induces cellular enhances function. However, mechanism subsequent regulation remain poorly understood. In present study, we show inhibition gap...
A systems pharmacological approach that capitalizes on the characterization of intracellular signaling networks can transform our understanding human diseases and lead to therapy development. Here, we applied this strategy identify targets for treatment Stargardt disease, a severe juvenile form macular degeneration. Diverse GPCRs have previously been implicated in neuronal cell survival, crosstalk between GPCR pathways represents an unexplored avenue intervention. We focused receptor family...
Summary There is growing evidence that micro RNA s are important regulators of gene expression in a variety cell types. Using immortalized lines and primary neural crest explants, we show ‐211, previously implicated the regulation melanoma proliferation invasiveness, promotes pigmentation melanoblasts melanocytes. Expression this regulated by key melanocyte transcription factor MITF regulates targeting TGF ‐ β receptor 2. Transfection with pre‐miR‐211 precursor molecules melb‐a melan‐a cells...
Rhodopsin homeostasis is tightly coupled to rod photoreceptor cell survival and vision. Mutations resulting in the misfolding of rhodopsin can lead autosomal dominant retinitis pigmentosa (adRP), a progressive retinal degeneration that currently untreatable. Using cell-based high-throughput screen (HTS) identify small molecules stabilize P23H-opsin mutant, which causes most cases adRP, we identified novel pharmacological chaperone opsin, YC-001. As non-retinoid molecule, YC-001 demonstrates...
Photoreceptor death leads to vision impairment in several retinal degenerative disorders. Therapies protecting photoreceptor from degeneration remain be developed. Anti-inflammation, anti-oxidative stress, and neuroprotective effects of celastrol have been demonstrated a variety disease models. The current study aimed investigate the protective effect celastrol. Bright light-induced BALB/c mice was used, morphological, functional, molecular changes retina were evaluated absence presence...
Oxidative stress (OS) induces osteoblast apoptosis, which plays a crucial role in the initiation and progression of osteoporosis. Although OS is closely associated with mitochondrial dysfunction, detailed mechanisms underlying OS-induced apoptosis have not been thoroughly elucidated to date. In present study, we found that abnormalities largely contributed as evidenced by enhanced production reactive oxygen species; considerable reduction respiratory chain complex activity, membrane...
Abstract Epithelial-to-mesenchymal transition (EMT) is a critical and complex process involved in normal embryonic development, tissue regeneration, tumor progression. It also contributes to retinal diseases, such as age-related macular degeneration (AMD) proliferative vitreoretinopathy (PVR). Although absent melanoma 2 (AIM2) has been linked inflammatory disorders, autoimmune cancers, its role the EMT of pigment epithelium (RPE-EMT) diseases remains unclear. The present study demonstrated...
ABSTRACT Dysfunction and loss of the retinal pigment epithelium (RPE) are hallmarks degeneration, but underlying pathogenetic processes only partially understood. Using mice with a null mutation in transcription factor gene Mitf, which RPE deficiencies associated we evaluated role trophic factors secreted by homeostasis. In such mice, thickness outer nuclear layer (ONL) is as wild type up to postnatal day 10, then progressively reduced, marked increase number apoptotic cells decline staining...
Abstract Background Oxidative stress is a common cause of neurodegeneration and plays central role in retinal degenerative diseases. Heme oxygenase-1 (HMOX1) redox-regulated enzyme that induced neurodegenerative diseases acts against oxidative but can also promote cell death, phenomenon still unexplained molecular terms. Here, we test whether HMOX1 has opposing effects during degeneration investigate the mechanisms behind its pro-apoptotic role. Methods Basal levels retinas are examined...
Virus-like particle (VLP) vaccine is considered to be the most promising candidate alternative traditional inactivated for foot-and-mouth disease (FMD). To elicit a desired immune response, hollow mesoporous silica nanoparticles (HMSNs) have been synthesized and utilized as nanocarrier FMD VLP delivery. The as-prepared HMSNs displayed relatively small size (∼260 nm), large cavity (∼150 thin wall (∼55 nm). inherent structural superiorities make them ideal nanocarriers vaccine, which exhibited...
J. Neurochem. (2012) 121 , 146–156. Abstract All‐ trans ‐retinal and its condensation‐products can cause retinal degeneration in a light ‐ dependent manner contribute to the pathogenesis of human macular diseases such as Stargardt’s disease age related degeneration. Although these toxic retinoid by products originate from rod cone photoreceptor cells, contribution each cell type induced is unknown. In this study, primary objective was learn whether rods or cones are more susceptible induced,...
Abstract Regeneration of the visual pigment by cells retinal epithelium (RPE) is fundamental to vision. Here we show that microphthalmia-associated transcription factor, MITF, which plays a central role in development and function RPE cells, regulates expression two cycle genes, Rlbp1 encodes retinaldehyde binding protein-1 (RLBP1) Rdh5 , retinol dehydrogenase-5 (RDH5). First, found are downregulated optic cups presumptive RPEs Mitf -deficient mouse embryos. Second, experimental manipulation...
Autophagy is an evolutionarily conserved catabolic mechanism that relieves cellular stress by removing/recycling damaged organelles and debris through the action of lysosomes. Compromised autophagy has been implicated in many neurodegenerative diseases, including retinal degeneration. Here we examined phenotypes resulting from RPE-specific deletion regulatory gene Atg7 generating Atg7flox/flox;VMD2-rtTA-cre+ mice to determine whether essential for RPE functions retinoid recycling....
Abstract Epithelial–mesenchymal transition (EMT) of the retinal pigment epithelium (RPE) is a hallmark pathogenesis proliferative vitreoretinopathy (PVR) that can lead to severe vision loss. Nevertheless, precise regulatory mechanisms underlying PVR remain largely unknown. Here, we show expression death-associated protein-like 1 (DAPL1) downregulated in membranes and DAPL1 deficiency promotes EMT RPE cells mice. In fact, adeno-associated virus (AAV)-mediated overexpression Dapl1- deficient...
Purpose: Dry eye disease (DED) is a multifactorial that associated with inflammation. Excessive DNA present in the tear fluid of patients DED. Absent melanoma 2 (AIM2) key sensor. This study aimed to investigate role AIM2 pathogenesis Methods: DED was induced by injection scopolamine (SCOP). Aberrant detected cell-free (cfDNA) ELISA and immunostaining. Corneal epithelial defects were assessed corneal fluorescein staining, zonula occludens-1 immunostaining TUNEL. Tear production analyzed...
Cited2 is a transcriptional modulator with pivotal roles in different biological processes. Cited2-deficient mouse embryos manifested two major defects the developing eye. An abnormal corneal-lenticular stalk was characteristic of Cited2(-/-) eyes, feature reminiscent Peters' anomaly, which can be rescued by increased Pax6 gene dosage embryonic eyes. In addition, hyaloid vascular system showed hypercellularity consisting aberrant vasculature, might correlated VEGF expression lens. Deletion...
Chicken plumage colour is a complex trait controlled by many genes. Herein, through Rhode Island Red (RIR) and White Leghorn (WL) F1 cross populations, the segregation of color was observed in females, showing white males, dark red (DR) light yellow (LY) females. The has been found to be caused dominant alleles (
Herein, we purposed to explore the association of sleep duration with chest pain among adults in US.This research work enrolled 13,274 subjects National Health and Nutrition Examination Survey (NHANES) from 2011 2018. The US was evaluated by Multivariable logistic regression.To elucidate association, made adjustments for gender, BMI, diabetes, smoking status, drinking race, marital annual family income, hyperlipoidemia, Hypertension. Chest incidence decreased 5% [OR = 0.95 (0.93, 0.98), p...
The decreased antioxidant capacity in the retinal pigment epithelium (RPE) is hallmark of degenerative diseases including age-related macular degeneration (AMD). Nevertheless, exact regulatory mechanisms underlying pathogenesis degenerations remain largely unknown. Here we show mice that deficiencies Dapl1, a susceptibility gene for human AMD, impair RPE and lead to 18-month-old homozygous partial deletion Dapl1. Dapl1-deficiency associated with reduction RPE's capacity, experimental...