A5074825225- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Antibiotic Use and Resistance
- Antibiotic Resistance in Bacteria
- Gut microbiota and health
- Influenza Virus Research Studies
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- Microscopic Colitis
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 detection and testing
- Neonatal and Maternal Infections
- Clinical Nutrition and Gastroenterology
- Diphtheria, Corynebacterium, and Tetanus
- Animal Disease Management and Epidemiology
- Bacterial Infections and Vaccines
- Clinical Laboratory Practices and Quality Control
- Mycobacterium research and diagnosis
- Antibiotics Pharmacokinetics and Efficacy
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
Bio-Rad (United States)
2022-2024
University of Michigan
2012-2021
Michigan Medicine
2010-2021
Michigan State University
2018
Michigan Department of Health and Human Services
2018
Henry Ford Hospital
2018
Mayo Clinic
2018
Lakeland Health
2018
Geriatric Research Education and Clinical Center
2013
Oklahoma Foundation for Medical Quality
2013
Integration of rapid diagnostic testing via matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) with antimicrobial stewardship team (AST) intervention has the potential for early organism identification, customization antibiotic therapy, and improvement in patient outcomes. The objective this study was to assess impact combined approach on clinical therapy-related outcomes patients bloodstream infections.A pre-post quasi-experimental conducted analyze MALDI-TOF AST...
The efficacy of influenza vaccines may decline during years when the circulating viruses have antigenically drifted from those included in vaccine.We carried out a randomized, double-blind, placebo-controlled trial inactivated and live attenuated healthy adults 2004-2005 season estimated both absolute relative efficacies.A total 1247 persons were vaccinated between October December 2004. Influenza activity Michigan began January 2005 with circulation an type A (H3N2) virus,...
Perennial and intense malaria transmission (holoendemic malaria) Epstein-Barr virus (EBV) infection are 2 cofactors in the pathogenesis of endemic Burkitt lymphoma (eBL). In present study, we compared EBV loads children living regions Kenya with differing intensities: Kisumu District, where is holoendemic, Nandi sporadic. For comparison, blood samples were also obtained from US adults, Kenyan patients eBL. Extraction DNA quantification by polymerase chain reaction give an load estimate that...
Previous studies have suggested a central role for superantigen-induced immune responses in the pathogenesis of streptococcal toxic shock syndrome. The production superantigens by clinical group A (GAS) isolates was studied, and ability plasma collected from patients with severe invasive GAS infections to neutralize proliferative- cytokine-inducing activities these investigated. Overnight culture supernatants all obtained disease were found contain superantigenic activity, as evident their...
Background. Studies of Clostridium difficile outbreaks suggested that certain ribotypes (eg, 027 and 078) cause more severe disease than other ribotypes. A growing number studies challenge the validity this hypothesis. Methods. We conducted a cross-sectional study C. infection (CDI) to test whether ribotype predicted clinical severity when adjusted for influence predictors. Toxigenic isolates were cultured from stool samples, screened genes encoding virulence factors by polymerase chain...
Background. The rationale and lessons learned through the evolution of National Survey for Susceptibility Bacteroides fragilis Group from its initiation in 1981 2007 are reviewed here. survey was conceived 1980 to track emerging antimicrobial resistance species.
The intestinal microbiome represents a complex network of microbes that are important for human health and preventing pathogen invasion. Studies examine differences in microbial communities across individuals with without enteric infections useful identifying support or impede health.16S rRNA gene sequencing was conducted on stool DNA from patients (n = 200) 75 healthy family members to identify community composition. Stools 13 were also examined post-infection better understand how recover....
Abstract A novel H1N1 influenza virus emerged in April 2009, and rapidly reached pandemic proportions. We report a retrospective observational case study of pathologic findings 8 patients with fatal infection at the University Michigan Health Systems (Ann Arbor) compared age-, sex-, body mass index–, treatment-matched control subjects. Diffuse alveolar damage (DAD) acute organizing phases affected all was accompanied by bronchopneumonia 6 patients. Organizing DAD established fibrosis present...
Studies evaluating rapid diagnostic testing plus stewardship intervention have consistently demonstrated improved clinical outcomes for patients with bloodstream infections. However, the cost of implementing new can be significant, and such usually does not generate additional revenue. There are minimal data impact adding matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) organism identification dedicating pharmacy personnel time on total hospital costs....
Streptococcal pyrogenic exotoxin F (SpeF), previously referred to as mitogenic factor, is a newly described potent mitogen produced by group A streptococci. To investigate whether this protein belongs the family of microbial superantigens, we analyzed cellular and molecular requirements for its presentation T cells compared it with known streptococcal superantigen (SpeA) nonspecific polyclonal T-cell phytohemagglutinin (PHA). SpeF SpeA were efficiently presented autologous antigen-presenting...
The efficacy of influenza vaccines may vary annually. In 2004-2005, when antigenically drifted viruses were circulating, a randomized, placebo-controlled trial involving healthy adults showed that inactivated vaccine appeared to be efficacious, whereas live attenuated less so.In 2005-2006, we continued our trial, examining the absolute and relative efficacies in preventing laboratory-confirmed symptomatic influenza.A total 2058 persons vaccinated October November 2005. Studywide activity was...
Inappropriate antibiotic use is a major factor contributing to the emergence and spread of antimicrobial resistance. The long turnaround time (over 24 hours) required for clinical susceptibility testing (AST) often results in patients being prescribed empiric therapies, which may be inadequate, inappropriate, or overly broad-spectrum. A reduction AST enable more appropriate therapies earlier. Here we report on new diagnostic asynchronous magnetic bead rotation (AMBR) biosensor droplet...
Rapid diagnostic testing with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) decreases the time to organism identification by 24 36 h compared amount required conventional methods. However, there are limited data evaluating impact MALDI-TOF real-time antimicrobial stewardship team (AST) review and intervention on prescribing outcomes for patients bacteremia blood cultures contaminated coagulase-negative Staphylococcus (CoNS). A quasiexperimental study was conducted...
Tigecycline is one of the few remaining therapeutic options for extensively drug-resistant (XDR) Gram-negative bacilli (GNB). MICs tigecycline to Acinetobacter baumannii have been reported be elevated when determined by Etest compared determinations broth microdilution (BMD) method. The study aim was compare susceptibility GNB four different testing methods. were collected from six health care systems (25 hospitals) in southeast Michigan January 2010 September 2011. among A. baumannii,...
The Klebsiella pneumoniae carbapenemase (KPC) was detected in carbapenem-resistant isolates of Citrobacter freundii and oxytoca recovered from different patients a Michigan hospital. Restriction analysis hybridization with KPC-specific probe showed the bla(KPC-2) genes these two genera family Enterobacteriaceae are carried on common plasmid.
Bacterial antibiotic resistance is one of the major concerns modern healthcare worldwide, and development rapid, growth-based, antimicrobial susceptibility tests key for addressing it. The cover image shows a self-assembled asynchronous magnetic bead rotation (AMBR) biosensor developed rapid detection bacterial growth. Using biosensors, minimum inhibitory concentration clinical E. coli isolate can be measured within two hours, where currently take 6-24 hours. A 16-well prototype also...
To establish the maximum-tolerated dose (MTD) and define toxicities of a single-dose infusion PNU-214565, recombinant Escherichia coli-derived fusion protein Staphylococcal enterotoxin A (SEA) Fab-fragment C242 monoclonal antibody in patients with advanced colorectal pancreatic carcinomas. investigate capability PNU-214565 to induce superantigen (SAg) response resulting cytokine production tumor regression.Twenty-one (age range, 39 76 years; median, 64; 12 men, nine women; 18 colorectal,...