In order to characterize the expression of genes associated with immune response mechanisms mastitis, we quantified relative IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF- α in milk cells healthy cows clinical mastitis. Total RNA was extracted from six Black White Holstein (BW) Gyr cows, including three animals without mastitis per breed. Gene analyzed by real-time PCR. IL-10 gene higher group BW compared free infection both breeds (p < 0.05). It also 0.001), but it not significant when were...

Abstract Background Heat stress (HS) poses significant threats to the sustainability of livestock production. Genetically improving heat tolerance could enhance animal welfare and minimize production losses during HS events. Measuring phenotypic indicators response understanding their genetic background are crucial steps optimize breeding schemes for improved climatic resilience. The identification genomic regions candidate genes influencing traits interest, including variants with...

This study was conducted to evaluate the effects of dietary glutamine levels on performance and carcass traits broilers kept in thermoneutral environment from 21 42 days age. Hundred sixty eight male Cobb® with age initial body weight 900 ± 4.71 g were allotted a completely randomized design four (0.00, 0.50, 1.00 1.50%), seven replicates six birds each. Performance, relative weights carcass, breast, thigh drumstick mRNA expression HSP70 evaluated. Birds housed environmental chambers...

Mycoplasma hyopneumoniae is the etiologic agent of enzootic pneumonia, which causes important economic losses to swine industry. The Toll-like receptors (TLRs) are pattern-recognition detect microbial presence and initiate innate as well adaptative immune defense. receptor 6 a type I transmembrane protein that recognizes bacterial components. aim this study was compare mRNA expression pattern TLR6 gene in two genetically distinct groups pigs vaccinated against hyopneumoniae.For each genetic...

Current assay evaluates thermoregulatory characteristics of native sheep in pregnancy and supplemented grazing system. Were used 24 multiparous lactating ewes, 12 Santa Inês Morada Nova, with an average weight 52.6 31.3 kg, respectively, were randomly distributed a completely randomized split-split plot design. Physiological parameters such as heart rate (HR), respiratory (RR) rectal temperature (RT) measured that order during the morning afternoon, 06-07 13-14 h, every seven days, totaling...

Objetivou-se mapear QTL nos cromossomos 5, 7 e 8 associá-los a características de carcaça, cortes qualidade carne, desempenho órgãos internos suínos. Uma progênie F2 614 animais foi produzida do cruzamento dois varrões da raça naturalizada brasileira Piau 18 fêmeas comerciais (Landrace x Large White Pietrain). A população genotipada para 14 marcadores microssatélites cobrindo os 8. Em seguida, construído o mapa ligação cada cromossomo. As análises associação foram feitas usando mapeamento...

Crude glycerin has been used as an alternative energy source in animal feeding and can thus change both the animal’s performance levels of gene expression related to production process antioxidant system, since effect depends on its degree purity diet. The aim this study was evaluate crude inclusion (0, 6 12%) diet characteristics mRNA abundance genes associated with mitochondrial function (uncoupling protein (UCP), cytochrome C oxidase subunit III (COX III) adenine nucleotide transporter...

The aim of this work was to evaluate the coefficient variation (CV) and establish classification ranges for main variables evaluated in experimentation with poultrys through different methods. CV data response observed poultry articles (broilers, laying hens, roosters quails) published five national journals were tabulated a Microsoft Excel 2010® worksheet. Afterwards, they subjected normality evaluation Shapiro-Wilk method, later following descriptive statistics utilized: maximum value,...

Aiming to reduce feed costs, cottonseed oil (CSO) has been used as an alternative component in diets for broilers. However, this contains gossypol, antinutritional agent that impacts the use of mineral elements, inhibits glucose uptake, and a direct inhibitory action on intestinal enzymes. Nevertheless, toxic effects gossypol can be prevented by addition iron salts, such ferrous sulfate (FS), diet. This work was conducted evaluate performance gene expression antioxidant enzymes superoxide...

Objetivou-se com este estudo mapear locos de características quantitativas (QTL) nos cromossomos 9, 10 e 11 suínos (Sus scrofa) associar seus efeitos em carcaça, cortes órgãos vísceras, desempenho qualidade carne. Utilizaram-se amostras DNA animais pertencentes a uma população F2, oriunda do cruzamento entre machos da raça Piau fêmeas Landrace õ Large White Pietrain. Um total 13 microssatélites foi utilizado na construção dos mapas ligação atual. As análises associação foram feitas...

1. Sodium bicarbonate (NaHCO3), potassium carbonate (K2CO3), and ammonium chloride (NH4Cl) are commonly used to correct dietary electrolyte balance (DEB) in birds. However, there many gaps the knowledge of their effects when simultaneously. This study investigated effect DEB levels on performance, femur bone characteristics expression genes related ionic concentrations broilers at 21 days age.2. Male Cobb broiler chickens (n = 245), aged 1-21 d, were divided into groups based a completely...

A realização do presente estudo teve como objetivo mapear Quantitative Trait Loci (QTL) de carcaça e qualidade carne em uma população F2 suínos desenvolvida pelo cruzamento dois reprodutores da raça brasileira Piau com 18 fêmeas comerciais (Landrace x Large White Pietrain). O mapa ligação para essa foi construído após a genotipagem 684 animais 35 marcadores microssatélites. Os dados foram analisados mapeamento por intervalo usando-se sexo, lote genótipo halotano efeitos fixos peso ao abate,...

Efficient conversion of food into body mass has been associated with altered gene expression some proteins the electron transport chain.We evaluated effect age on mRNA uncoupling protein, adenine nucleotide translocase and cytochrome c oxidase complex III in quails.One-day-old broiler quails were sacrificed after 7, 14, 21, or 28 days.Six each slaughtered by cervical dislocation.Total RNA was extracted from breast muscle reverse transcribed cDNA.Real-time PCR analysis performed using...

Compreender o comportamento do consumidor de carne ovina permite um entendimento assertivo das demandas e preferências mercado. Este estudo visou caracterizar perfil no município Mossoró, localizado estado Rio Grande Norte. Para tanto foi utilizada uma metodologia pesquisa tipo Survey. A coleta dados realizada através questionário online, contendo 35 questões fechadas, cobrindo aspectos sociodemográficos consumo. Os resultados mostraram que a maioria dos consumidores é sexo feminino,...

Mapeou-se quantitative trait loci (QTL) associados a características de desempenho nos cromossomos 1, 2, 3, 12, 14, 15 e X suínos pertencentes uma população F2, formada partir do cruzamento entre dois machos da raça naturalizada brasileira Piau 18 fêmeas comerciais (Landrace x Large White Pietrain). O mapa genético ligação foi construído após genotipagem dos animais para 35 marcadores microssatélites. As estimativas conteúdo informação polimórfica indicaram que os microssatélites foram...

The objective of this study was to evaluate the chemical composition and fermentative parameters heart palm waste produced from Alexander Palm, ensiled with additives. Treatments consisted silage sheath or without calcium oxide. In oxide, we evaluated control (without additive) enriched 5.0 g kg−1 urea (urea). oxide (control) Experimental silos were distributed in a completely randomized design 2 × factorial arrangement (inclusion lack lime inclusion urea), four replicates. Crude protein...

Research on the use of heart palm by-product (HPB) as a feed for dairy cattle is needed since it low-cost alternative in addition avoiding environmental contamination. The objective this study was to evaluate intake, digestibility, milk yield and composition from cows fed HPB. Sixteen Holstein were used. Treatments consisted fresh sugarcane replacement (dry matter basis – DM) HPB at levels 0%, 7.5%, 15% 22.5%. Experimental design randomized blocks with measures repeated time, data compared...

Background: Leptospirosis is a cosmopolitan zoonosis caused by pathogenic spirochetes of the genus Leptospira spp. and it considered one main causes reproductive problems in cattle. Therefore, aim this study was to determine occurrence anti-Leptospira antibodies identify prevalent serovars risk factors associated with infection cattle herds, microregion Floriano, Piaui State, Brazil.Materials, Methods &amp; Results: A total 414 bovine sera samples were collected (390 females aged over 24...