Background Chickens are animals that sensitive to thermal stress, which may decrease their production level in terms it affects feed intake and thus, decreasing body weight gain. The Heat Shock Factors (HSF) Proteins (HSP) genes involved the key cellular defense mechanisms during exposure hot environments. Aimed with this study analyze expression of HSF1, HSF3, HSP70 HSP90 two local breeds (Peloco Caneluda) a commercial broiler line (Cobb 500®) verify differences resistance these chicken...

Frequencies of κ-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Guzerat Nellore and x Holstein crossbreds) by means polymerase chain reaction-restriction fragment length polymorphism analysis using two independent restriction nucleases (Hinf I HaeIII).The genotyping (A B) is practical importance, since B allele found to correlate with commercially valuable parameters cheese yielding efficiency.The frequencies among...

Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (control [CTL]; fed 1.0 times the maintenance requirement; n = 12; and overnourished [ON]; at 1.5 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows slaughtered 135, 190, 240 d gestation samples LM collected for analysis mRNA expression histological evaluation collagen content number cells. There was no interaction between gestational period nutrition variables...

Thirteen reference genes were investigated to determine their stability be used as a housekeeping in gene expression studies skeletal muscle of chickens. Five different algorithms for ranking and results suggested that individual rankings the differed among them. The validated using samples obtained from Pectoralis major chicken. Samples chickens development periods post hatch under nutritional diets. For calculation ΔΔCt approach was applied compare relative pairs within each 52 when...

Quantitative RT-PCR is an important technique for assessing gene expression. However, a proper normalization of reference genes prior to expression analyses target necessary. The best normalizer that which remains stable in all samples from different treatments. aim this study was identify muscle tissue three genetically divergent chickens groups (Peloco, Cobb 500® and Caneluda) under environmental (heat stress comfort) sex influence. Expressions ten were tested stability breast muscular...

The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action its alleles immune functions, whereas indirect production traits might explained by better general conditions more productive animals. In present study, BoLA-DRB3 gene was investigated in 1058 cows belonging seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total 37 were identified, 15 them described for first time breed. highly significant association (p < 0.02)...

1. A study was conducted to test the hypothesis that feed efficiency (FE) correlated with expression of genes from somatotropic axis and mitochondrial involved in energy production, environment which birds are exposed influenced such genes.2. Quails were divided into High-FE low-FE groups maintained a comfortable or heat stress (HS) (38°C for 24 h) evaluate changes insulin-like growth factor I (IGF-I), hormone receptor (GHR), adenine nucleotide translocator (ANT), uncoupling protein (UCP)...

The PSS genotypes of 596 F2 pigs produced by initial mating Brazilian native boars commercial sows and were characterized PCR-RFLP their carcass performance traits evaluated. Among the animals analyzed, 493 (82.72%) as NN 103 (17.28%) Nn. With respect to traits, Nn presented higher (p < 0.05) right half weight, left loin depth eye area, lower shoulder backfat thickness, thickness between last next but one lumbar vertebrae after rib at 6.5 cm from midline compared animals. also showed values...

Este trabalho foi realizado para estimar a diversidade genética de 30 acessos capim-elefante utilizando-se marcadores moleculares. Amostras DNA genômico cada acesso foram obtidas realização da amplificação por PCR utilizando técnica RAPD. Foram utilizados 20 primers aleatórios que geraram 88 bandas alta intensidade e reprodutíveis (64 polimórficas 24 monomórficas). Os agrupados pelo método UPGMA com base na estimativa das distâncias genéticas. grupos Porto Rico, Santa Rita IJ-7136 cv. EMPASC...

The components of the insulin-like growth factor (IGF) system appear to be involved in regulation ovarian follicular and atresia pig. We investigated expression pattern mRNAs for IGF1 (IGF1), its binding proteins (IGFBP1, IGFBP2, IGFBP3, IGFBP5), epidermal swine follicle cells tissue throughout estrous cycle using real-time quantitative PCR technique. results gene were analyzed linear regression with as a dependent variable days an independent variable. Additionally, analysis was made...

The aim of this study was to evaluate the effect dietary lysine on performance, protein deposition and respiratory chain gene expression in male broilers. A total 252 Cobb 500 broilers were distributed, a completely randomized design, into four treatments with seven replicates nine birds per experimental unit. Experimental consisted diets based corn soybean meal, levels digestible lysine: 1.016%, 1.099%, 1.182% 1.265%. increase level diet provided higher weight gains, feed efficiency body...

Abstract: The objective of this work was to evaluate the tillering dynamics 'Suvernola' digit grass (Digitaria eriantha) when subjected different defoliation frequencies. Four frequencies (interval among harvests) were evaluated - very high, reached 25 cm in height; 35 cm; medium, 45 and low, 55 two seasons year, with higher lower insolation, from January 2015 2016. A randomized complete block design four replicates used. increase frequency increased number generations appearance, mortality,...

Follicular dynamics and the expression of candidate genes using real-time polymerase chain reaction (PCR) were compared during oestrous cycle pig breeds with high (commercial line; n = 24) low (local Brazilian Piau; 21) ovulation rates prolificacy. Gilts killed on Days 0, 4, 10 18 visible ovarian follicles classified by follicular diameter. Recovered cumulus–oocyte complexes as normal or atretic frozen in liquid nitrogen until RNA extraction. Low and/or prolificacy Piau gilts was associated...

The bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative control this parasite. We examined the pattern of gene expression three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen d3 (S100A7), calcium channel transient receptor potential vanilloid 6 (TRPV6), and cysteine proteinase...

Em 2006, Jeannette Wing publicou um artigo descrevendo o Pensamento Computacional (PC) como conjunto de habilidades necessárias ao século XXI. Desde então, muitas pesquisas na área da educação e computação relatam formas introduzir PC no ensino. Este apresenta mapeamento sistemático feito com objetivo identificar artigos que abordem maneira este contribua para a aprendizagem disciplinas básica. Resultados apontam há pouca pesquisa envolvendo forma interdisciplinar. No entanto, estas poucas...

In this study, we analyzed insulin-like growth factor I (IGF-I), hormone receptor (GHR) and uncoupling protein (UCP) mRNA expression in the muscle liver of high- (0.23 g/g) low- (0.17 feed-efficiency (FE) Japanese quail at three different air temperatures: comfortable (25 °C), heat stress (38 °C) for 12 h or cold (10 h. Total RNA was extracted from breast each quail, cDNA amplified using specific primers target genes. Expression quantitative real-time PCR (qRT-PCR). IGF-I higher livers...

Uma população F2 de suínos obtida a partir do intercruzamento da geração F1, proveniente acasalamento divergente dois machos raça naturalizada brasileira Piau com 18 fêmeas comerciais (Landrace × Large White Piétrain) e genotipada para cinco marcadores tipo microssatélite foi utilizada o objetivo mapear locos características quantitativas associados desempenho no cromossomo 4. As avaliadas foram: número tetas, peso ao nascimento, aos 21, 42, 63, 77 105 dias idade, abate, consumo ração,...

Summary Pig is an important animal for meat production; this generally associated with characteristics determined prenatally during myogenesis. Expressed sequence tags ( EST ) can provide direct information on the transcriptome and indirect relation between genome phenotype, giving about differentially expressed genes DEG ). In work, identification annotation of from libraries three pig breeds D uroc, L arge W hite ocal B reed P iau) were performed followed by real‐time PCR analyses pre‐...

MicroRNAs are a class of naturally occurring non-coding RNAs. Typically they ∼22 nucleotides long and suppress translation their targets genes. Several laboratories have attempted to identify miRNAs from pig muscle the bioinformatics strategies using ESTs proved be successful for this aim. In study we report an in silico identification ncRNA EST libraries focusing on novel further investigated differential expression pigs (known novel) by quantitative real-time PCR during pre- postnatal...