A5003580258- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Cardiovascular Health and Disease Prevention
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Advanced Glycation End Products research
- Endoplasmic Reticulum Stress and Disease
- Cerebrovascular and Carotid Artery Diseases
- Adipose Tissue and Metabolism
- Peripheral Artery Disease Management
- Genetics and Neurodevelopmental Disorders
- Regulation of Appetite and Obesity
- Diabetic Foot Ulcer Assessment and Management
- Liver Disease Diagnosis and Treatment
- Cardiovascular Function and Risk Factors
- Pancreatitis Pathology and Treatment
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Retinal Diseases and Treatments
- Pituitary Gland Disorders and Treatments
- Natural Antidiabetic Agents Studies
- Medication Adherence and Compliance
- Thyroid Disorders and Treatments
Wakayama Medical University
2021-2025
Keio University
2004-2023
Osaka University
2013-2022
Suita Municipal Hospital
2018
Tokushima University
2014
Weatherford College
2008
Hiroshima University
2007
Vanderbilt University
2004-2005
Kanazawa Medical University
2005
Kanazawa University
2005
Abstract Neurofilament light chain (NfL) is a novel biomarker of neurodegenerative diseases. It detectable in the peripheral blood, allowing low-invasive assessment early signs neurodegeneration. The level NfL gradually increases with age; however, what other factors affect it remains unclear. present study examined association between blood and renal function among healthy participants undergoing health check (n = 43, serum NfL) patients diabetes mellitus 188, plasma NfL). All were 60 years...
The C1/RIPE3b1 (−118/−107 bp) binding factor regulates pancreatic-β-cell-specific and glucose-regulated transcription of the insulin gene. In present study, activator from mouse βTC-3 cell nuclear extracts was purified by DNA affinity chromatography two-dimensional gel electrophoresis. activity found in roughly 46-kDa fraction at pH 7.0 4.5, each contained N- C-terminal peptides to MafA as determined peptide mass mapping tandem spectrometry. detected complex using peptide-specific antisera....
Insulin gene expression is regulated by several islet-enriched transcription factors. However, MafA the only β cell-specific activator. Here, we show that selectively induces endogenous insulin in non-β cells. was also first detected insulin-producing cells formed during second and predominant phase of cell differentiation, absent few insulin-positive found Nkx6.1 -/- pancreata, which lack majority second-phase These results demonstrate a potent activator likely to function downstream islet...
The c-Jun N-terminal kinase (JNK) pathway is known to be activated under diabetic conditions and possibly involved in the progression of insulin resistance. In this study, we examined effects modulation JNK liver on resistance glucose tolerance. Overexpression dominant-negative type obese mice dramatically improved markedly decreased blood levels. Conversely, expression wild normal sensitivity. phosphorylation state crucial molecules for signaling was altered upon modification pathway....
The glycolytic enzyme glucokinase plays a primary role in the glucose-responsive secretion of insulin, and defects this can cause NIDDM. As step toward understanding molecular basis (GK) gene regulation, we assessed structure regulation human GK beta-cell-type promoter. results reporter analyses using HIT-T15 cells revealed that promoter was comprised multiple cis-acting elements, including two primarily important cis-motifs: palindrome structure, hPal-1, insulin cis-motif A element-like...
The pancreatic β- and α-cells are developmentally related to each other but reveal diverse gene expression patterns. Among the two important transcription factors for insulin expression, IEF1 is present both in α- β-cells, PDX-1/IPF1/STF-1/IDX-1, a homeodomain-containing factor, β-cells not α-cells. To elucidate function of PDX-1 β-cell-specific genes, we established stable αTC1 clone 6 (αTC1.6)-derived transfectants expressing examined changes patterns them. exogenous αTC1.6 cells alone...
To determine the impact of blood glucose profile, involving fluctuation and excursion levels, on glycated proteins, we evaluated association among daily profile glucose, albumin (GA) HbA1c levels in patients with type 1 diabetes (n = 93) 2 75). GA were strongly correlated (r 0.85, P<0.0001) 0.61, P<0.0001), respectively. similar between diabetes, while significantly higher diabetes. Thus ratio to was than that (3.32 0.36 vs. 2.89 0.44, p<0.001). The degrees maximum mean Stepwise multivariate...
Diabetes is the most prevalent and serious metabolic disease, number of diabetic patients worldwide increasing. The reduction insulin biosynthesis in pancreatic β-cells closely associated with onset progression diabetes, thus it important to search for ways induce insulin-producing cells non−β-cells. In this study, we showed that a modified form duodenal homeobox factor 1 (PDX-1) carrying VP16 transcriptional activation domain (PDX-1/VP16) markedly increases induces various pancreas-related...
SARS-CoV-2 infection could trigger autoimmune disease. We report a case of concomitant exacerbation Graves orbitopathy (GO) and myasthenia gravis (MG) after infection. A 43-year-old woman had diplopia, proptosis, swollen eyelids. Blood tests showed thyrotoxicosis positive thyroid-stimulating hormone receptor antibodies, orbital magnetic resonance imaging (MRI) enlarged extraocular muscles. She was therefore referred to our hospital with diagnosis GO. Methylprednisolone pulse therapy (MPT) in...
OBJECTIVE—The binding of advanced glycation end products (AGEs) to their receptor (RAGE) plays an important role in the development diabetic vascular complications. In present study, we examined circulating endogenous secretory RAGE (esRAGE) levels subjects with type 1 diabetes and explored possible association between esRAGE severity RESEARCH DESIGN AND METHODS—Circulating serum were 67 Japanese patients (22 men 45 women, age 24.0 ± 4.4 years [means SD]) 23 age-matched healthy nondiabetic...
The transcription factor pancreatic and duodenal homeobox 1 (PDX-1) is expressed in progenitor cells. In exocrine pancreas, PDX-1 down-regulated during late development, while re-up-regulation of has been reported cancer pancreatitis. To determine whether sustained expression could affect pancreas was constitutively all lineages by transgenic approaches. markedly small with the replacement acinar cells duct-like structures, accompanied activated Stat3. Genetic ablation Stat3 profoundly...
MafA, a recently isolated pancreatic beta-cell-specific transcription factor, is potent activator of insulin gene transcription. In this study, we show that MafA overexpression, together with PDX-1 (pancreatic and duodenal homeobox factor-1) NeuroD, markedly increases expression in the liver. Consequently, substantial amounts protein were induced by such combination. Furthermore, streptozotocin-induced diabetic mice, overexpression liver, dramatically ameliorated glucose tolerance, while...
Reactive oxygen species (ROS) are induced under diabetic conditions and likely associated with the development of type 2 diabetes. It is also known that ROS production facilitated in presence copper ion through Fenton reaction. The aim this study was to examine involvement pathogenesis diabetes evaluate potential usefulness a chelating agent for treatment First, both serum levels C57BL/KsJ-db/db mice were significantly higher compared those nondiabetic mice. Second, we treated db/db...
OBJECTIVE Islet β-cells loose their ability to synthesize insulin under diabetic conditions, which is at least partially due the decreased activity of transcription factors such as MafA. Although an in vitro study showed that reactive oxygen species (ROS) decrease MafA expression, underlying mechanism still remains unclear. In this study, we examined effects c-Jun, known be upregulated by ROS, on expression conditions. RESEARCH DESIGN AND METHODS To examine protein levels and performed...
Brachial-ankle pulse wave velocity (baPWV) is a method to estimate arterial stiffness, which reflects the stiffness of both aorta and peripheral artery; it would be applicable general practice, since its measurementis automated. The aim this study was evaluate whether baPWV can predictors future cardiovascular events (CVE) in diabetic patients. We prospectively evaluated association between or carotid intima-media thickness (carotid IMT) at baseline new onset CVE 1040 type 2 patients without...