A5069270299- Plant Molecular Biology Research
- RNA Research and Splicing
- Plant Reproductive Biology
- RNA and protein synthesis mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- RNA modifications and cancer
- Light effects on plants
- Plant nutrient uptake and metabolism
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Plant Physiology and Cultivation Studies
- Immunodeficiency and Autoimmune Disorders
- Polysaccharides and Plant Cell Walls
- Genomics and Chromatin Dynamics
- Vitamin K Research Studies
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Lipoproteins and Cardiovascular Health
- Genomics and Rare Diseases
- Immune Cell Function and Interaction
- Soybean genetics and cultivation
- Genetic Associations and Epidemiology
- Mast cells and histamine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Masaryk University
2020-2024
Centrum kardiovaskulární a transplantační chirurgie
2018-2024
Central European Institute of Technology
2016-2019
Central European Institute of Technology – Masaryk University
2016-2019
Mendel University in Brno
2007-2017
Czech Academy of Sciences
2001-2009
Czech Academy of Sciences, Institute of Biophysics
2001-2009
Postembryonic de novo organogenesis represents an important competence evolved in plants that allows their physiological and developmental adaptation to changing environmental conditions. The phytohormones auxin cytokinin (CK) are regulators of the fate pluripotent plant cells. However, molecular nature interaction(s) control is largely unknown. Here, we show CK modulates auxin-induced (AIO) via regulation efflux-dependent intercellular distribution. We used hypocotyl explants-based vitro...
Abstract The development and activity of the procambium cambium, which ensure vascular tissue formation, is critical for overall plant architecture growth. However, little known about molecular factors affecting meristems formation. Here, we show that His kinase CYTOKININ-INDEPENDENT1 (CKI1) cytokinin receptors ARABIOPSIS HISTIDINE KINASE2 (AHK2) AHK3 are important regulators in Arabidopsis thaliana shoots. Genetic modifications CKI1 cause dysfunction two-component signaling pathway defects...
Abstract The dioecious white campion Silene latifolia (syn. Melandrium album) has heteromorphic sex chromosomes, XX in females and XY males, that are larger than the autosomes enable their separation by flow sorting. group of MROS genes, first male-specifically expressed genes plants, was recently identified S. latifolia. To localize we used flow-sorted X chromosomes as a template for PCR with internal primers. Our results indicate MROS3 gene is located at least two copies tandemly arranged...
High concentrations of cytokinins (CKs) in the cultivation medium can induce partial photomorphogenesis dark-grown Arabidopsis seedlings. However, no significant increases endogenous CK levels have been found de-etiolated mutants, suggesting that either parallel pathways are involved light and responses, or changes sensitivity to CKs occur during photomorphogenesis. Here it is shown even modest induced by transgenic expression biosynthetic gene, ipt, lead many typical features light-induced...
Common variable immunodeficiency disorders (CVID) represent a group of primary diseases characterized by hypogammaglobulinemia and impaired specific Ab response, resulting in recurrent infections due to dysfunctional immune response. The mechanisms mediating deficiency CVID remain be determined. Previous studies indicated that dysregulation patients is associated with chronic microbial translocation, systemic activation, altered homeostasis lymphocytic myeloid lineages. A detailed...
Abstract Hereditary angioedema due to C1 inhibitor deficiency (C1-INH-HAE) is a rare and life-threatening condition characterized by recurrent localized edema. We conducted systematic screening of SERPING1 defects in cohort 207 Czech patients from 85 families with C1-INH-HAE. Our workflow involved combined strategy sequencing extended UTR deep intronic regions, advanced silico prediction tools, mRNA-based functional assays. This approach allowed us detect causal variant all except one...
Complex cardiovascular procedures may initiate a systemic inflammatory response syndrome (SIRS) with massive cytokine release, which is involved in postoperative myocardial injury. Intraoperative hemoadsorption (HA) mitigates the response. Micro ribonucleic acids (miRNAs) are emerging as marker of injury.This study evaluated if intraoperative reduction by HA modulates SIRS and affects injury measured miRNA-126, 223 miRNA-1, 133a, respectively. Twenty-eight patients were assigned into (n =...
Plant hormones control plant development by modulating the expression of regulatory genes, including homeobox-containing KNOXI genes. However, much remains to be elucidated about interactions involved. Therefore, hormonal regulation gene was investigated using hormone applications and an inducible transgenic ipt system increase endogenous cytokinin (CK) levels. Treatments with auxin, abscisic acid (ABA), cytokinins, ethylene, gibberellin (GA) did not result in ectopic BP (BREVIPEDICELLUS)...
Acceptor splice site recognition (3′ site: 3′ss) is a fundamental step in precursor messenger RNA (pre-mRNA) splicing. Generally, the U2 small nuclear ribonucleoprotein (snRNP) auxiliary factor (U2AF) heterodimer recognizes 3′ss, of which U2AF35 has dual function: (i) It binds to intron–exon border some 3′ss and (ii) mediates enhancer-binding splicing activators’ interactions with spliceosome. Alternative mechanisms for have been suggested, yet they are still not thoroughly understood. Here,...
Splicing-affecting mutations can disrupt gene function by altering the transcript assembly. To ascertain splicing dysregulation principles, we modified a minigene assay for parallel high-throughput evaluation of different next-generation sequencing. In our model system, all exonic and six intronic positions SMN1 gene's exon 7 were mutated to possible nucleotide variants, which amounted 180 unique single-nucleotide mutants 470 double mutants. The resulted in wide range aberrations. Exonic...
Neutrophils impact on processes preceding the formation of bradykinin, a major swelling mediator in hereditary angioedema (HAE), yet their potential role HAE pathogenesis has not been sufficiently studied. We assessed relative mRNA expression 10 genes related to neutrophil activation using RNA extracted from peripheral blood neutrophils 23 patients symptom-free period and 39 healthy donors. Increased levels CD274, IL1B, IL1RN, IL8, MMP9, TLR4, together with lack mutual correlations detected...
Mutations can be induced by environmental factors but also arise spontaneously during DNA replication or due to deamination of methylated cytosines at CpG dinucleotides. Sites where mutations occur with higher frequency than would expected chance are termed hotspots while sites that contain rarely coldspots. permanently scanned and repaired repair systems. Among them, the mismatch targets base pair mismatches, which discriminated from canonical pairs probing altered elasticity DNA. Using...
Abstract Tandem donor splice sites (5′ss) are unique regions with at least two GU dinucleotides serving as splicing cleavage sites. The Δ3 tandem 5′ss a specific subclass of separated by 3 nucleotides which can affect protein function inserting/deleting single amino acid. One is typically preferred, yet factors governing particular choice not fully understood. A highly conserved exon 21 the STAT3 gene was chosen model to study mechanisms. Based on multiple lines experimental evidence,...
ABSTRACT Purpose In 2015, the American College of Medical Genetics and Genomics (ACMG) Association for Molecular Pathology (AMP) published consensus standardized guidelines variant classification in Mendelian disorders. To increase accuracy consistency, Clinical Genome Resource (ClinGen) Familial Hypercholesterolemia (FH) Variant Curation Expert Panel (VCEP) was tasked with optimizing existing ACMG/AMP framework disease-specific FH. Here, we provide recommendations most common FH-causing...