A5046963687- Mast cells and histamine
- Immune Cell Function and Interaction
- Food Allergy and Anaphylaxis Research
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Erythrocyte Function and Pathophysiology
- Kruppel-like factors research
- Acne and Rosacea Treatments and Effects
- Hemoglobinopathies and Related Disorders
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- melanin and skin pigmentation
- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- Bladder and Urothelial Cancer Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Transgenic Plants and Applications
Université Toulouse III - Paul Sabatier
2021-2024
Centre de Recherche en Cancérologie de Toulouse
2021-2024
Inserm
2019-2024
Université de Toulouse
2021-2024
Centre National de la Recherche Scientifique
1996-2024
Nantes Université
2019
Université d'Angers
2019
Université de Reims Champagne-Ardenne
1996
BackgroundDespite their central role in peanut allergy, human monoclonal IgE antibodies have eluded characterization.ObjectiveWe sought to define the sequences, affinities, clonality, and functional properties of allergy.MethodsWe applied our single-cell RNA sequencing–based SEQ SIFTER discovery platform samples from allergic individuals who varied by age, sex, ethnicity, geographic location order understand commonalities response allergens. Select were then recombinantly expressed...
Mast cells (MCs) are multifaceted innate immune often present in the tumor microenvironment (TME). However, MCs have been only barely characterized studies focusing on global infiltrate phenotyping. Consequently, their role cancer is still poorly understood. Furthermore, prognosis value confusing since associated with good and bad (or both) depending type. In this pilot study performed a surgical cohort of 48 patients Non-Small Cell Lung Cancer (NSCLC), we MC population within TME matching...
Acne is a multifactorial disease driven by physiological changes occurring during puberty in the pilosebaceous unit (PSU) that leads to sebum overproduction and dysbiosis involving notably Cutibacterium acnes . These PSU microenvironment lead shift from homeostatic an inflammatory state. Indeed, immunohistochemical analyses have revealed inflammation lymphocyte infiltration can be detected even infraclinical acneic stages, highlighting importance of early stages disease. In this study, we...
Abstract Effector CD4+ T (Teff) lymphocytes infiltrate sites of inflammation and orchestrate the immune response by instructing local leukocytes. Mast cells (MCs) are tissue sentinel strategically located near blood vessels cell–rich areas. MC/Teff cell interactions shape Teff responses, but in turn, action on MCs is still poorly understood. Here, we analyzed human interplay through both application RNA sequencing functional assays. We showed that activated induce a specific transcriptomic...
Interleukin-33 (IL-33) is an alarmin released by epithelial cells in response to tissue damage. It activates resident immune sentinel cells, which then produce signals commonly associated with type 2 responses, particularly affecting infiltrating antigen-specific T cells. Given that mast (MCs) are a primary target of IL-33 and can shape helper (Th) cell we investigated the effect priming on ability MCs influence Th cytokine production. To examine cell/MC interaction, developed human MC/memory CD4
ABSTRACT Background Diagnosis of allergies is mostly based on the patient's clinical history and allergen provocation tests. Determination specific IgE (sIgE) profiles can be performed to support allergy diagnosis. This commonly done in vivo by skin prick test or vitro with automated systems. Several platforms exist quantify sIgE levels, but all these methods require access instruments, often delaying test's results. The luciferase‐linked immunosorbent assay (LuLISA) allows bioluminescent...
The actions of transforming growth factor β (TGFβ) and erythropoietin (Epo) were studied using normal erythroid progenitors from fetal rat liver spleen at 18, 19 20 days. rhTGFβ1 inhibited the late BFUe colonies significantly each age in both organs methylcellulose cultures containing 2 U/ml rhEpo. There was no significant inhibition CFUe proliferation, except for 18 days, suggesting different sensitivities to factors a given age, spleen. colorimetric MTT assay used examine human leukemic...
ABSTRACT Acne is a multifactorial disease driven by physiological changes occurring during puberty in the pilosebaceous unit (PSU) that leads to sebum overproduction and dysbiosis involving notably Cutibacterium acnes . These PSU microenvironment lead shift from homeostatic an inflammatory state. Indeed, immunohistochemical analyses have revealed inflammation lymphocyte infiltration can be detected even infraclinical acneic stages, highlighting importance of early stages disease. In this...
Summary CD4 + T helper cells (Th) infiltrate sites of inflammation and orchestrate the immune response by instructing local leukocytes. Mast (MCs) are tissue sentinel particularly abundant in skin mucosa. Here, we analyzed interplay between human MCs Th and, through application RNAseq functional assays, showed that induced a specific transcriptomic program helped (named here MC TH ) driving them toward an inflammatory phenotype. The gene signature indicated cell acquired turn capacity to...
e14592 Background: A better understanding of the immune-modulating interactions between tumor cells and immune underlying balance control resistance in colorectal cancer (CRC) is crucial for design immunotherapies. We have previously demonstrated that overexpression human MHC class Ib molecule - HLA-E/β2 microglobulin by CRC was associated with an unfavorable prognosis, suggesting its involvement escape. However, specific receptor HLA-E/β2m CD94/NKG2A, inhibitory or CD94/NKG2C, activating...
The actions of transforming growth factor β (TGFβ) and erythropoietin (Epo) were studied using normal erythroid progenitors from fetal rat liver spleen at 18, 19 20 days. rhTGFβ1 inhibited the late BFUe colonies significantly each age in both organs methylcellulose cultures containing 2 U/ml rhEpo. There was no significant inhibition CFUe proliferation, except for 18 days, suggesting different sensitivities to factors a given age, spleen. colorimetric MTT assay used examine human leukemic...