A5081001323- Neuroblastoma Research and Treatments
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Radiopharmaceutical Chemistry and Applications
- Fuel Cells and Related Materials
- Genetics and Neurodevelopmental Disorders
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Protease and Inhibitor Mechanisms
- Cardiac Structural Anomalies and Repair
- Protein Degradation and Inhibitors
- Arctic and Russian Policy Studies
- Peroxisome Proliferator-Activated Receptors
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Virus-based gene therapy research
Inserm
2017-2025
Institut Curie
2017-2025
Université Paris Sciences et Lettres
2017-2025
La Ligue Contre le Cancer
2025
Université Toulouse-I-Capitole
2009
Abstract Noradrenergic and mesenchymal identities have been characterized in neuroblastoma cell lines according to their epigenetic landscapes core regulatory circuitries. However, relationship relative contribution patient tumors remain poorly defined. We now document spontaneous reversible plasticity between the two identities, associated with reprogramming, several models. Interestingly, xenografts cells from each identity eventually harbor a noradrenergic phenotype suggesting that...
Abstract Although heterogeneity of FAP+ Cancer-Associated Fibroblasts (CAF) has been described in breast cancer, their plasticity and spatial distribution remain poorly understood. Here, we analyze trajectory inference, deconvolute transcriptomics at single-cell level perform functional assays to generate a high-resolution integrated map cancer (BC), with focus on inflammatory myofibroblastic (iCAF/myCAF) CAF clusters. We identify 10 spatially-organized CAF-related cellular niches, called...
Abstract Two cell identities, noradrenergic and mesenchymal, have been characterized in neuroblastoma lines according to their epigenetic landscapes relying on specific circuitries of transcription factors. Yet, relationship relative contribution patient tumors remain poorly defined. Here, we demonstrate that the knock-out GATA3 , but not PHOX2A or PHOX2B cells induces a mesenchymal phenotype. Our results document spontaneous plasticity several models between both identities show relies...
Abstract Long intergenic non-coding RNAs (lincRNAs) are emerging as integral components of signaling pathways in various cancer types. In neuroblastoma, only a handful lincRNAs known upstream regulators or downstream effectors oncogenes. Here, we exploit RNA sequencing data primary neuroblastoma tumors, neuroblast precursor cells, cell lines and cellular perturbation model systems to define the lincRNome map up- driver genes MYCN , ALK PHOX2B . Each these controls expression particular...
Abstract In contrast to adult cancers, most pediatric malignancies display limited genetic heterogeneity. Ewing sarcoma, the major driver of tumor development is expression chimeric EWSR1-FLI1 transcription factor, which results from a chromosome translocation. Additional recurrent alterations include CDKN2A, STAG2, and TP53 genes. Through its binding specific elements including GGAA microsatellite sequences, has impact on epigenetic landscape cancer cells consequently, obscures makeup...
Abstract Through the analysis of neuroblastoma super-enhancer landscape, we could recently identy core regulatory circuitries (CRC) that drive identity, revealing two main types identity: a noradrenergic identity defined by CRC module including PHOX2B, HAND2, and GATA3, neural crest cell (NCC)-like driven containing AP-1 transcription factors (Boeva et al., Nature Genet 2017). We observed heterogeneous SK-N-SH line exhibited mixed whereas SH-SY5Y SH-EP lines, subcloned from parental line,...
Abstract Introduction: The heterogeneity of neuroblastoma cell identity has been recently revealed with the characterization noradrenergic and NCC (neural crest cell)-like/mesenchymal cells, each type being governed by a core regulatory circuitry (CRC) specific transcription factors (Boeva et al., 2017; van Groningen 2017). Whereas CRC includes PHOX2B, HAND2, GATA3 factors, associated to NCC-like/mesenchymal is composed AP-1 among others. present study aims at deciphering role...