A5019796877- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Neuropeptides and Animal Physiology
- Photoreceptor and optogenetics research
- Growth Hormone and Insulin-like Growth Factors
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Protein Degradation and Inhibitors
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Photochromic and Fluorescence Chemistry
- Peptidase Inhibition and Analysis
- DNA and Nucleic Acid Chemistry
- Adipose Tissue and Metabolism
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Signaling Pathways in Disease
- Cancer Cells and Metastasis
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Regulation of Appetite and Obesity
- Cancer Research and Treatments
- Advanced Fluorescence Microscopy Techniques
Centre National de la Recherche Scientifique
2016-2025
Sorbonne Université
2012-2025
Université Paris Cité
2012-2025
Institut de Biologie de l'École Normale Supérieure
2015-2025
Université Paris Sciences et Lettres
2016-2025
École Normale Supérieure - PSL
2016-2025
Laboratoire de Physique de l'ENS
2017-2025
École Normale Supérieure
2024-2025
Université Paris-Panthéon-Assas
2023
Sorbonne Paris Cité
2016-2022
We have previously demonstrated that the envelope proteins of a murine and primate retrovirus are immunosuppressive in vivo. This property was manifested by ability proteins, when expressed allogeneic tumor cells normally rejected engrafted mice, to env-expressing escape (at least transiently) immune rejection. Here, we analyzed activity human syncytins. These genes from endogenous retroviruses independently coopted ancestral hosts, conserved evolution, specifically placenta, with cell-cell...
Abstract Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate functions as well homeostasis interface. To date, astroglial mechanisms underlying these have been poorly addressed. Here we demonstrate a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, pool bound to ribosomes, endfeetome, primarily encodes for secreted membrane proteins. detected nascent protein synthesis Finally, determined...
Alterations of chromatin modifiers are frequent in cancer, but their functional consequences often remain unclear. Focusing on the Polycomb protein EZH2 that deposits H3K27me3 (trimethylation Lys27 histone H3) mark, we showed its high expression solid tumors is a consequence, not cause, tumorigenesis. In mouse and human models, dispensable for prostate cancer development restrains breast High results from tight coupling to proliferation ensure homeostasis. However, this process malfunctions...
Persistent luminescence nanoparticles (PLNPs) are attracting growing interest for non-invasive optical imaging of tissues with a high signal to noise ratio. PLNPs can emit persistent through the tissue transparency window several minutes, after UV light excitation before systemic administration or directly in vivo visible irradiation, allowing us get rid autofluorescence tissues. constitute promising alternative commercially available near infrared probes thanks their versatile...
We previously delineated a highly conserved immunosuppressive (IS) domain within murine and primate retroviral envelope proteins (Envs). The envelope-mediated immunosuppression was manifested by the ability of proteins, when expressed allogeneic tumor cells normally rejected engrafted mice, to allow these escape, at least transiently, immune rejection. Using this approach, we identified key residues whose mutation specifically abolishes IS activity without affecting “mechanical” fusogenic...
Male androgenetic alopecia (AGA) is the most common form of hair loss in men. It characterized by a distinct pattern progressive starting from frontal area and vertex scalp. Although several genetic risk loci have been identified, relevant genes for AGA remain to be defined.To identify biomarkers associated with AGA.Molecular premature were identified through gene expression analysis using cDNA generated scalp biopsies hairless or bald men AGA, healthy volunteers.This monocentric study...
Why does a normal cell possibly harboring genetic mutations in oncogene or tumor suppressor genes becomes malignant and develops is subject of intense debate. Various theories have been proposed but their experimental test has hampered by the unpredictable improbable transformation single cells. Here using an optogenetic approach we permanently turn on (KRASG12V) zebrafish brain that, only synergy with transient co-activation reprogramming factor (VENTX/NANOG/OCT4), undergoes deterministic...
Why does a normal cell possibly harboring genetic mutations in oncogene or tumor suppressor genes becomes malignant and develops is subject of intense debate. Various theories have been proposed but their experimental test has hampered by the unpredictable improbable transformation single cells. Here, using an optogenetic approach we permanently turn on (KRASG12V) zebrafish brain that, only synergy with transient co-activation reprogramming factor (VENTX/NANOG/OCT4), undergoes deterministic...
The insulin-like growth factor (IGF) system is a major regulator of somatic in vertebrates. Both ligands (IGF-I and IGF-II) signal via the same IGF receptor (IGF-IR). Classical IGF-IR invalidation lethal at birth, so that conditional models are needed to study postnatal role this receptor. To establish genetically inducible IGF-IR, we targeted gene using construct introduced neomycin resistance cassette into intron 2, leaving rest intact. This interfered with processing primary transcript,...
Abstract Reduced IGF type I receptor levels diminish postnatal growth rate and adult body weight in mice. Here, we studied the impact of experimental deficiency on tissue-specific by Cre-lox-mediated dosage a floxed IGF-IR gene. We generated mice with wide spectrum (5–82%), separated them into two groups either strong (≥50%) (XS mice) or moderate (<50%, M mice). The XS was significantly retarded from 3 wk after birth onward, respect to littermates. This effect twice as males females....
To improve our knowledge of the bioactive conformation CCK-B antagonists, we have developed a new series constrained dipeptoids whose synthesis and biochemical properties are reported here. These compounds, general structure N alpha-[(2-adamantyloxy)carbonyl]-alpha-methyltryptophanyl-(4 -X)-proline, were designed by introducing cyclization in previously described CCK-B/peptoid antagonist RB 210, N-[N-[(2-adamantyloxy)carbonyl]-DL-alpha-methyltryptophanyl] -N-(2-phenylethyl)glycine (Blommaert...
Abstract Tumor development is a multistep process in which both genetic and epigenetic events cooperate for the emergence of malignant clone. The possibility that endogenous retroviruses promote expansion neoplastic clone by subverting immune surveillance has been proposed, but remained elusive. Here we show knocking down—by RNA interference—an retrovirus spontaneously induced B16 murine melanoma results rejection tumor cells immunocompetent mice, under conditions where control grow into...
Most RecQ DNA helicases share a conserved domain arrangement that mediates their activities in genomic stability. This comprises helicase motor domain, C-terminal (RecQ-C) region including winged-helix (WH) and 'Helicase RNase D C-terminal' (HRDC) domain. Single-molecule real-time translocation unwinding by full-length Escherichia coli variants lacking either the HRDC or both WH domains was analyzed. operated under two interconvertible kinetic modes, 'slow' 'normal', as it unwound duplex...
The biological processes associated with the onset of schizophrenia remain largely unknown. Current hypotheses favor gene × environment interactions as supported by our recent report about DNA methylation changes during psychosis. Here, we conducted first longitudinal transcriptomic analysis blood samples from 31 at-risk individuals who later converted to psychosis and 63 did not. Individuals were followed for a maximum 1 year. Blood collected at baseline end follow-up served their own...
Somitogenesis, the segmentation of antero-posterior axis in vertebrates, is thought to result from interactions between a genetic oscillator and posterior-moving determination wavefront. The segment (somite) size set by product period velocity Surprisingly, while can vary factor three 20 °C 32 °C, somite constant. How this temperature independence achieved mystery that we address study. Using RT-qPCR show endogenous fgf8 mRNA concentration decreases during somitogenesis correlates with...
Inserting a simple mask in microscope enables 3D tracking of single cells and subnanometer force spectroscopy.
The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate typical human oncogene, kRASG12V, embryos investigate developmental tumorigenic phenotypes. demonstrate spatiotemporal...