A5107423876- Barrier Structure and Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Connexins and lens biology
- Caveolin-1 and cellular processes
- Cancer-related molecular mechanisms research
- Bacterial Infections and Vaccines
- Neuroscience of respiration and sleep
- Neurological Disease Mechanisms and Treatments
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
- Retinal Development and Disorders
- Salivary Gland Disorders and Functions
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- Single-cell and spatial transcriptomics
- Pregnancy and preeclampsia studies
- Ophthalmology and Eye Disorders
- Cellular transport and secretion
- Olfactory and Sensory Function Studies
Centre Interdisciplinaire de Recherche en Biologie
2017-2022
Inserm
2017-2022
Collège de France
2017-2021
Centre National de la Recherche Scientifique
2017-2021
Université Paris Sciences et Lettres
2017-2021
Sorbonne Université
2017-2021
Laboratoire de Psychologie : Cognition, Comportement, Communication
2021
Centre de Gestion Scientifique
2020
Hôpital Lariboisière
2017
Assistance Publique – Hôpitaux de Paris
2017
Abstract Astrocytes send out long processes that are terminated by endfeet at the vascular surface and regulate functions as well homeostasis interface. To date, astroglial mechanisms underlying these have been poorly addressed. Here we demonstrate a subset of messenger RNAs is distributed in astrocyte endfeet. We identified, among this transcriptome, pool bound to ribosomes, endfeetome, primarily encodes for secreted membrane proteins. detected nascent protein synthesis Finally, determined...
Abstract Brain mural cells form a heterogeneous family which significantly contributes to the maintenance of blood-brain barrier and regulation cerebral blood flow. Current procedures isolate them cannot specifically separate their distinct subtypes, in particular vascular smooth muscle (VSMCs) mid-capillary pericytes (mcPCs), differ among others by expression actin (SMA). We herein describe an innovative method allowing SMA + VSMCs − mcPCs be freshly isolated from rat cortex. Using...
Absence of the astrocyte-specific membrane protein MLC1 is responsible for megalencephalic leukoencephalopathy with subcortical cysts (MLC), a rare type leukodystrophy characterized by early-onset macrocephaly and progressive white matter vacuolation that lead to ataxia, spasticity, cognitive decline. During postnatal development (from P5 P15 in mouse), forms complex GlialCAM (another astrocytic transmembrane protein) at junctions between perivascular processes. Perivascular processes along...
Astrocytes are the most abundant glial cells of central nervous system and have recently been recognized as crucial in regulation brain immunity. In neuropathological conditions, astrocytes prone to a radical phenotypical change called reactivity, which plays key role astrocyte contribution neuroinflammation. However, how regulate immunity healthy conditions is an understudied question. One astroglial molecule involved these regulations might be Connexin 43 (Cx43), gap junction protein...
Megalencephalic Leukoencephalopathy with subcortical Cysts (MLC) is a type of vacuolating leukodystrophy, which mainly caused by mutations in MLC1 or GLIALCAM. The two MLC-causing genes encode for membrane proteins yet unknown function that have been linked to the regulation different chloride channels such as ClC-2 and VRAC. To gain insight into role MLC proteins, we determined brain GlialCAM interacting proteome. proteome includes transporters ion known be involved homeostasis, related...
Streptococcus pneumoniae or pneumococcus (PN) is a major causative agent of bacterial meningitis with high mortality in young infants and elderly people worldwide. The mechanism underlying PN crossing the blood brain barrier (BBB) specifically, role non-endothelial cells neurovascular unit that control BBB function, remains poorly understood. Here, we show astroglial connexin 43 (aCx43), gap junctional component expressed astrocytes, plays predominant during meningitis. Following intravenous...
Abstract The brain dense vascular network is essential for distributing oxygen and nutrients to neural cells. develops during embryogenesis leads the formation of endothelial blood-brain barrier (BBB). This surrounded by mural cells (pericytes smooth muscle (VSMCs)) fibroblasts. Here, we compared molecular functional properties on postnatal day (P)5 vs. P15, via a transcriptomic analysis purified mouse cortical microvessels (MVs) identification vascular-cell-type-specific or -preferentially...
Abstract Although great efforts to characterize the embryonic phase of brain microvascular system development have been made, its postnatal maturation has barely described. Here, we compared molecular and functional properties vascular cells on day (P)5 vs. P15, via a transcriptomic analysis purified mouse cortical microvessels (MVs) identification vascular-cell-type-specific or -preferentially expressed transcripts. We found that endothelial (EC), smooth muscle (VSMC) fibroblasts (FB)...
Introductory paragraph Streptococcus pneumoniae or pneumococcus (PN) is a major causative agent of bacterial meningitis with high mortality in young infants and elderly people. The mechanism underlying PN crossing the blood brain barrier (BBB) remains poorly understood. Here, we show that gap junctional component connexin 43 expressed astrocytes (aCx43) plays role meningitis. Following intravenous challenge, mice deficient for aCx43 developed milder symptoms showed severely reduced counts...
Although great efforts to characterize the embryonic phase of brain vascular system development have been made, its postnatal maturation has barely described. Here, we compared molecular and functional properties cells on day (P)5 vs. P15, via a transcriptomic analysis purified mouse cortical microvessels (MVs) identification vascular-cell-type-specific or -preferentially expressed transcripts. We found that endothelial (EC), smooth muscle (VSMC) fibroblasts (FB) follow specific programs...
Abstract Absence of the astrocyte-specific membrane protein MLC1 is responsible for megalencephalic leukoencephalopathy with subcortical cysts (MLC); this rare type leukodystrophy characterized by early-onset macrocephaly and progressive white matter vacuolation that lead to ataxia, spasticity, cognitive decline. During postnatal development (from P5 P15 in mouse), forms a complex GlialCAM (another astrocytic transmembrane protein) at junctions between perivascular processes (PvAPs, which...