A5007254450- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hedgehog Signaling Pathway Studies
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Traditional Chinese Medicine Analysis
- Erythropoietin and Anemia Treatment
- Medicinal Plant Pharmacodynamics Research
- Retinal Diseases and Treatments
- Neonatal Health and Biochemistry
- Connexins and lens biology
- Chromatography in Natural Products
- Synthesis and Biological Evaluation
- Lipid metabolism and disorders
- Phytochemicals and Antioxidant Activities
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- Pediatric health and respiratory diseases
- Food Science and Nutritional Studies
- Hemoglobin structure and function
- Phytochemistry and biological activity of medicinal plants
- Analytical Methods in Pharmaceuticals
China Pharmaceutical University
2021-2024
Blood–brain barrier (BBB) prevents neurotoxins from entering central nervous system. We aimed to establish and characterize an in vitro triple co-culture BBB model consisting of brain endothelial cells hCMEC/D3, astrocytoma U251 cells, neuroblastoma SH-SY5Y cells. Co-culture markedly enhanced claudin-5 VE-cadherin expression hCMEC/D3 accompanied by increased transendothelial electrical resistance decreased permeability. Conditioned medium (CM) (S-CM), (U-CM), (US-CM) also promoted...
Blood-brain barrier (BBB) prevents neurotoxins from entering central nervous system. We aimed to establish and characterize an in vitro triple co-culture BBB model consisting of brain endothelial cells hCMEC/D3, astrocytoma U251 cells, neuroblastoma SH-SY5Y cells. Co-culture markedly enhanced claudin-5 VE-cadherin expression hCMEC/D3 accompanied by increased transendothelial electrical resistance decreased permeability. Conditioned medium (CM) (S-CM), (U-CM), (US-CM) also promoted...
Liver injury is often associated with hepatic retinopathy, resulting from accumulation of retinal toxins due to blood–retinal barrier (BRB) dysfunction. Retinal pigment epithelium highly expresses MRP1/Mrp1. We aimed investigate whether liver affects the function and expression Mrp1 using bile duct ligation (BDL) rats. distributions fluorescein 2,4-dinitrophenyl-S-glutathione were used for assessing function. BDL significantly increased two substrates bilirubin, downregulated protein,...
Blood–brain barrier (BBB) prevents neurotoxins from entering central nervous system. We aimed to establish and characterize an in vitro triple co-culture BBB model consisting of brain endothelial cells hCMEC/D3, astrocytoma U251 cells, neuroblastoma SH-SY5Y cells. Co-culture markedly enhanced claudin-5 VE-cadherin expression hCMEC/D3 accompanied by increased transendothelial electrical resistance decreased permeability. Conditioned medium (CM) (S-CM), (U-CM), (US-CM) also promoted...
Blood-brain barrier (BBB) prevents neurotoxins from entering central nervous system. We aimed to establish and characterize an in vitro triple co-culture BBB model consisting of brain endothelial cells hCMEC/D3, astrocytoma U251 cells, neuroblastoma SH-SY5Y cells. Co-culture markedly enhanced claudin-5 VE-cadherin expression hCMEC/D3 accompanied by increased transendothelial electrical resistance decreased permeability. Conditioned medium (CM) (S-CM), (U-CM), (US-CM) also promoted...
Blood-brain barrier (BBB) prevents neurotoxins from entering central nervous system. We aimed to establish and characterize an in vitro triple co-culture BBB model consisting of brain endothelial cells hCMEC/D3, astrocytoma U251 cells, neuroblastoma SH-SY5Y cells. Co-culture markedly enhanced claudin-5 VE-cadherin expression hCMEC/D3 accompanied by increased transendothelial electrical resistance decreased permeability. Conditioned medium (CM) (S-CM), (U-CM), (US-CM) also promoted...
The research on the processing mechanism of Chinese medicine is key and core foundation to improve technologies medicine, formulate quality standards medicinal pieces, enhance clinical efficacy enrich theories, promote development processing. Many researc-hers have conducted in-depth exploration in 20 years 21 st century. Significant progress has been made transformation chemical components during processing, change active body, law toxicity attenuation toxic enhancement with auxiliary...
N 6 -methyladenosine (m A) plays crucial roles in the regulation of RNA metabolisms. METTL16 identified as a single-component methyltransferase catalyzes formation m A nucleus, whether it regulates cytoplasmic fate remains unknown. Here, we detected dual localization nucleus and cytoplasm. depletion dramatically attenuates protein synthesis, but activity is not required for its translation-promoting function. Mechanistically, prominent interactor METTL16, eIF4E2, which represses translation...
Objective: Polygoni Multiflori Radix (PM) is a traditional herbal medicine with repeated reports of liver injury events in recent years. We wondered whether the classical processing method, namely, nine-time steaming and sun drying (NSSD), had toxicity-attenuating effects on PM relationships between toxicity times processing, as well alteration trends its compounds. Materials Methods: The chemical fingerprints different extracts were developed using ultra-high-performance liquid...
<p>Up-regulated genes</p>
<p>DAVID Functional annotation analysis</p>
<p>DAVID Functional annotation analysis</p>
<p>Taqman probes used in qPCR</p>
<p>Supplementary Figures and Legends</p>
BACKGROUND: Hemin, a stable form of heme iron, is potential iron supplement for the treatment deficiency. To date, pharmacokinetics and in vivo ADME properties hemin are to be elucidated.METHODS: In this study, rapid, sensitive, validated inductively coupled plasma mass spectrometry (ICP-MS) method was used combination with 58Fe isotope labeling systemically investigate pharmacokinetics, biodistribution, excretion, binding profiles animals.RESULTS: Results showed that ICP-MS accurate...