A5053287040- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Plant biochemistry and biosynthesis
- Political Influence and Corporate Strategies
- PI3K/AKT/mTOR signaling in cancer
- Regional Development and Environment
- Hydropower, Displacement, Environmental Impact
- China's Socioeconomic Reforms and Governance
- Translation Studies and Practices
- Scientific Computing and Data Management
- Cultural Heritage Materials Analysis
- HVDC Systems and Fault Protection
- Image Processing and 3D Reconstruction
- Heat shock proteins research
- Research Data Management Practices
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Cloud Computing and Remote Desktop Technologies
- Biological and pharmacological studies of plants
- Chromatography in Natural Products
- Autophagy in Disease and Therapy
China Pharmaceutical University
2020-2025
Beijing Forestry University
2023-2025
China Tobacco
2024
Fudan University
2021-2023
Sainsbury Laboratory
2023
University of Cambridge
2023
Qilu Hospital of Shandong University
2023
Peking University
2006-2022
Capital Medical University
2022
Shanghai University of Engineering Science
2010-2022
Abstract Dynamic mRNA modification in the form of N 6 -methyladenosine (m A) adds considerable richness and sophistication to gene regulation. The m A mark is asymmetrically distributed along mature mRNAs, with approximately 35% residues located within coding region (CDS). It has been suggested that methylation CDS slows down translation elongation. However, neither decoding feature endogenous mRNAs nor physiological significance clearly defined. Here, we found leads ribosome pausing a...
N6-methyladenosine (m6A) plays crucial roles in regulating RNA metabolisms. METTL16 identified as a single-component methyltransferase catalyzes m6A formation the nucleus; whether it regulates cytoplasmic fate remains unknown. Here, we detected dual localization of nucleus and cytoplasm. depletion attenuates protein synthesis, but activity is not required for its translation-promoting function. Mechanistically, an interactor METTL16, eIF4E2, which represses translation by acting competitor...
Lung cancer is one of the most common malignant diseases globally, composed non-small cell lung (NSCLC, 85%) and small (SCLC, 15%). MicroRNAs (miRNAs) are single-stranded noncoding RNAs having important roles in development. miR-411-5p/3p were reported to be increased significantly human NSCLC tissues lines. Moreover, overexpression could accelerate proliferation migration, impede apoptosis Mechanically, SPRY4 confirmed a direct target miR-411-5p/3p. Furthermore, our findings showed that...
N6-methyladenosine (m6A) has emerged as a crucial epitranscriptomic mark which regulates broad spectrum of physiological processes including stem cell differentiation. m6A-binding YTHDF proteins have recently been proposed to mediate differentiation leukemia in redundant manner. However, whether these play semblable roles pluripotent remain largely unknown. Here, we showed the differential functions YTHDF1 and YTHDF3 controlling embryonic cells (ESCs). Depletion ESCs resulted loss...
The CPP gene family comprises transcription factor genes containing a conserved CRC domain, which is mainly involved in plant development and evolution. Although have been widely studied many plants, little known about them woody especially the endangered species Phoebe bournei (Hemsl.). In genome of bournei, we identified 11 PbCPP (PbCPP1-PbCPP11) distributed on four chromosomes, with large differences number amino acids. They encode both acidic alkaline proteins. A phylogenetic analysis...
Mouse embryonic stem cells (ESCs) consist of a rare population heterogeneous 2-cell-like (2CLCs). These transiently recapitulate the transcriptional and epigenetic features 2-cell embryos, serving as unique model for studying totipotency acquisition development. Accumulating evidence has demonstrated that transcription factors modifications exert crucial functions in transition ESCs to 2CLCs. However, roles RNA modification regulation 2C-like state remain elusive. Using DUX-induced 2CLCs...
Arecoline, a biologically active alkaloid extracted from the areca nut, serves as primary psychoactive ingredient in betel quid, one of most widely consumed substances worldwide. Despite its extensive use, central nervous system (CNS) effects arecoline remain inadequately understood. This study aims to investigate actions through comprehensive, multi-dimensional approach that integrates behavioral assays, neuroimaging techniques, calcium signaling analysis, and transcriptomic profiling. Our...
Background: Recent evidence has indicated that long non-coding RNA (lncRNA) is involved in the pathogenesis of type 2 diabetes, but nothing known about lncRNA expression changes lymphatic endothelial cells response to diabetes. Methods: The GSE38396 dataset was downloaded from Gene Expression Omnibus database and probe sets Human Gnome U133 Plus2.0 microarray were annotated for lncRNA. Differentially expressed lncRNAs between diabetic non-diabetic calculated. Results: Compared with patients,...
N6-Methyladenosine (m6A) is the most abundant chemical modification occurring on eukaryotic mRNAs, and has been reported to be involved in almost all stages of mRNA metabolism. The distribution m6A sites notably asymmetric along with a strong preference toward 3' terminus transcript. How regional shaped remains incompletely understood. In this study, by performing m6A-seq chromatin-associated RNAs, we found that arises during transcription. Nucleosome occupancy remarkedly increased region...
Adipogenesis is mediated by the complex gene expression networks involving posttranscriptional modifications. The natural compound rhein has been linked to regulation of adipogenesis, but underlying regulatory mechanisms remain elusive. Herein, we systematically analyzed effects on adipogenesis at both transcriptional and levels. Rhein remarkably suppresses in stage-specific dose-dependent manners. identified inhibit fat mass obesity-associated (FTO) demethylase activity. Surprisingly,...
Effective and efficacious drug screening methods are imperative in the discovery process. A library has been constructed efficiently followed by situ cell-based without product purification. This method led to of several iminosugar derivatives with anticancer activity.
Abstract Background Diabetic wounds are refractory and very difficult to heal. We aimed use miRNA identify novel specific molecular markers for diabetes mellitus (DM) diagnosis treatment. Methods The expression level of miR‐296‐5p was determined in tissue samples 12 DM patients. effect on proliferation β‐cells examined using Cell Counting Kit‐8 (CCK‐8) colony formation assay. cell cycle progression analysed flow cytometry. target gene verified luciferase reporter A rat model used assess the...
The p53 tumor suppressor exerts antitumor functions through its ability to regulate the transcription of downstream targets. Long noncoding RNAs (lncRNAs) act as oncogenes or suppressors implicated in tumorigenesis and progression. Here, we identify lncRNA LINC00324 (long intergenic RNA 00324) a direct transcriptional target. Knockdown expression promotes growth by reducing activity, whereas ectopic demonstrates reverse effect. Notably, is present endogenous complex cells directly binds...
Ribosome pausing at start codons limits translational output, which is controlled by altered mRNA methylation in cancer cells.
MicroRNAs (miRNAs) are short (20-23 nt) non-coding RNAs that involved in post-transcriptional regulation of gene expression multicellular organisms by affecting both the stability and translation mRNAs. In recent years, deep sequencing transcription is being increasingly utilized with promise higher sensitivity for identification differential patterns as well opportunity to discover new transcripts, including alternative isoforms miRNAs. this study, miRNAs from A549 cells treated...
Background: Currently, there are few studies on the effect of prophylactic anti-hepatitis B virus (HBV) therapy (AVT) for mother-to-child transmission during pregnancy postpartum hepatitis flare (PHF) and risk factors in women with chronic infection. Aim: To analyze AVT related to flare. Methods: This study retrospectively enrolled surface antigen (HBsAg)-positive e (HBeAg)-positive HBV <math xmlns="http://www.w3.org/1998/Math/MathML"...