A5043475085- RNA Research and Splicing
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Polyomavirus and related diseases
- Retinoids in leukemia and cellular processes
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Insect Pest Control Strategies
- DNA and Nucleic Acid Chemistry
- Skin Protection and Aging
- Insect and Pesticide Research
- Fungal Biology and Applications
- Microbial Metabolites in Food Biotechnology
- Bioactive Compounds and Antitumor Agents
- Estrogen and related hormone effects
- Melanoma and MAPK Pathways
- Fungal and yeast genetics research
Kyungpook National University
2017-2025
Harvard University
2014-2016
Beth Israel Deaconess Medical Center
2014-2016
Beth Israel Deaconess Hospital
2013-2016
We have previously shown that RNA polymerase II (Pol II) pause release and transcriptional elongation involve phosphorylation of the factor TRIM28 by DNA damage response (DDR) kinases ATM DNA-PK. Here we report a significant role for breaks DDR signalling in mechanisms stimulus-inducible genes humans. Our data show enrichment γH2AX on serum-induced important function DNA-PK Pol activation-coupled these genes. accumulation decreases when P-TEFb is inhibited, confirming results from...
CDK7 associates with the 10-subunit TFIIH complex and regulates transcription by phosphorylating C-terminal domain (CTD) of RNA polymerase II (RNAPII). Few additional substrates are known. Here, using covalent inhibitor SY-351 quantitative phosphoproteomics, we identified kinase in human cells. Among hundreds high-confidence targets, vast majority unique to (i.e., distinct from other transcription-associated kinases), a subset that suggest novel cellular functions. Transcription-associated...
Hypoxic stress responses are crucial for cellular and organismal survival provoke gene regulation in diverse biological pathways including cell cycle progression energy metabolism. Here, we show that topoisomerase IIbeta (TOP2B) regulates DNA topology transcription hypoxia-inducible genes (HIGs) a DNA-PK-dependent manner. Cellular, mutational, genomic analyses showed an antagonistic relationship between TOP2B DNA-PK activities. associates with HIGs represses by suppressing negative...
ERK1 is a key kinase in the mitogen-activated protein pathway, which transduces extracellular signals into cellular responses. Following triggering of cytosolic phosphorylated and translocated nucleus to activate DNA-bound factors target genes. This study reports novel role human as DNA endonuclease catalyze single-strand breaks without sequence-specificity. catalysis required magnesium ion was independent ATP. Biochemical structural analyses indicated that N-terminal domain allosterically...
Methyl benzoate (MB) is a small, hydrophobic organic compound isolated from the freshwater fern Salvinia molesta (Salviniales: Salviniaceae). It used as fragrance and flavor enhancer owing to its pleasant smell. has also demonstrated potential green pesticide for various groups of insects. However, effects on mites are yet be studied.Here, we assessed acaricidal repellent MB against two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), under laboratory greenhouse...
The function of the mitogen-activated protein kinase signaling pathway is required for activation immediate early genes (IEGs), including EGR1 and FOS, cell growth proliferation. Recent studies have identified topoisomerase II (TOP2) as one important regulators transcriptional IEGs. However, mechanism underlying regulation involving TOP2 in IEG has remained unknown. Here, we demonstrate that ERK2, but not ERK1, report a critical ELK1 binding sequence ERK2 at gene. Our data indicate both ERK1...
Arsenic is reportedly a biphasic inorganic compound for its toxicity and anticancer effects in humans. Recent studies have shown that certain arsenic compounds including hexoxide (AS4O6; hereafter, AS6) induce programmed cell death cycle arrest human cancer cells murine models. However, the mechanisms by which AS6 suppresses are incompletely understood. In this study, we report of through transcriptome analyses. particular, cytotoxicity global gene expression regulation were compared normal...
RNA polymerase II (Pol II)-dependent transcription in stimulus-inducible genes requires topoisomerase IIβ (TOP2B)-mediated DNA strand break and the activation of damage response signalling humans. Here, we report a novel function breast cancer 1 (BRCA1)-BRCA1-associated ring domain (BARD1) complex this process. We found that BRCA1 is phosphorylated at S1524 by kinases ataxia-telangiectasia mutated ATR during gene activation, event important for productive transcription. Our biochemical...
Methyl benzoate (MB) is a small, hydrophobic organic compound that isolated from the freshwater fern, Salvinia molesta. Because of its pleasant odor, it has been used as fragrance and flavor enhancer. In addition, to attract orchid bees for pollination in farm tested potential be developed green pesticide targeting diverse group insects. spite wide applications, safety MB humans remains poorly understood. this study, we cytotoxicity against cultured human cells, including kidney, colon,...
The increasing importance of environmental sustainability has led to the development new materials that are environmentally friendly, functional, and cost-effective. Lignin-containing cellulose nanomaterials a common example these. advantages lignocelluloses include their renewability, sustainability, functionality combined with molecular rigidity enhanced hydrophobicity. In order valorize these beneficial traits from lignin-containing nanocellulose, various approaches have been examined in...
Many noncoding RNAs (ncRNAs) serve as regulatory molecules in various physiological pathways including gene expression mammalian cells. Distinct from protein-coding RNA expression, ncRNA is regulated solely by transcription and processing/stability. Transcriptional regulation genes thus important to be understood yet known completely. Previously, we identified that a subset of transcriptionally polymerase II (Pol II) promoter-proximal pausing tissue-specific manner. In this study, human are...
Abstract Hypoxic responses have been implicated in critical pathologies, including inflammation, immunity, and tumorigenesis. Recently, efforts to identify effective natural remedies health supplements are increasing. Previous studies reported that the cell lysates wall-bound lipoteichoic acids of Lactiplantibacillus plantarum K8 (K8) exert anti-inflammatory immunomodulative effects. However, effect on cellular hypoxic remains unknown. In this study, we found had a potent suppressive gene...
Glyphosate is the most widely used herbicide worldwide. However, since International Agency for Research on Cancer (IARC) classified glyphosate as a Class 2A 'probable carcinogen' to humans, safety of this synthetic chemical has been under debate and requires validation. Most studies have conducted in animals, effects humans are not fully understood. In present study, we report that increases rate cell growth human embryonic kidney 293 (HEK293) cells. Interestingly, concentrations ranging...
The maintenance of lysosomal integrity is essential for lysosome function and cell fate. Damaged lysosomes are degraded by autophagy, lysophagy. mechanism underlying lysophagy remains largely unknown; this study aimed to contribute the understanding topic. A cell-based screening system was used identify novel modulators. Triamterene (6-phenylpteridine-2,4,7-triamine) identified as one most potent inducers from process. We found that triamterene causes rupture without affecting other cellular...