A5058287566- Tissue Engineering and Regenerative Medicine
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- Mesenchymal stem cell research
- CAR-T cell therapy research
- Diabetes Management and Research
- Electrospun Nanofibers in Biomedical Applications
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- Neurological disorders and treatments
- RNA Interference and Gene Delivery
- Drug Solubulity and Delivery Systems
- Cardiac pacing and defibrillation studies
- Cannabis and Cannabinoid Research
- Advancements in Transdermal Drug Delivery
- Neuroscience and Neural Engineering
- Advanced Drug Delivery Systems
- Traumatic Ocular and Foreign Body Injuries
Houston Methodist
2020-2025
University of Chinese Academy of Sciences
2022-2024
Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing Encapsulation (NICHE) device, which integrates direct vascularization facile mass transfer localized immunosuppressant delivery rejection prophylaxis. Here, investigated NICHE allogeneic long-term reversal in an immunocompetent, male rat model. We...
The impact of pre-exposure prophylaxis (PrEP) on slowing the global HIV epidemic hinges effective drugs and delivery platforms. Oral drug regimens are pillar PrEP, but variable adherence has spurred development long-acting systems with aim increasing PrEP access, uptake, persistence. We have developed a subcutaneous nanofluidic implant that can be refilled transcutaneously for sustained release islatravir, nucleoside reverse transcriptase translocation inhibitor is used PrEP. In rhesus...
Abstract Cell delivery and encapsulation platforms are under development for the treatment of Type 1 Diabetes among other diseases. For effective cell engraftment, these require establishing an immune‐protected microenvironment as well adequate vascularization oxygen supply to meet metabolic demands therapeutic cells. Current rely on 1) immune isolating barriers indirect or 2) direct with local systemic modulatory molecules. Supported by experimental data, here a broadly applicable...
Cell encapsulation is an attractive transplantation strategy to treat endocrine disorders. Transplanted cells offer a dynamic and stimulus-responsive system that secretes therapeutics based on patient need. Despite significant advancements, challenge in allogeneic cell maintaining sufficient oxygen nutrient exchange, while providing protection from the host immune system. To this end, we developed subcutaneously implantable dual-reservoir integrating situ prevascularization local...
Abstract The current standard for cell encapsulation platforms is enveloping cells in semipermeable membranes that physically isolate transplanted from the host while allowing oxygen and nutrient diffusion. However, long‐term viability function of encapsulated are compromised by insufficient supply to graft. To address this need, a strategy achieve enhanced vascularization 3D‐printed, polymeric platform using platelet‐rich plasma (PRP) mesenchymal stem (MSCs) investigated. study conducted...
Immune checkpoint inhibitors (ICI), pembrolizumab and atezolizumab, were recently approved for treatment-refractory triple-negative breast cancer (TNBC), where those with Programmed death-ligand 1 (PD-L1) positive early-stage disease had improved responses. ICIs are administered systemically in the clinic, however, reaching effective therapeutic dosing is challenging due to severe off-tumor toxicities. As such, intratumoral (IT) injection increasingly investigated as an alternative delivery...
Several implantable long-acting (LA) delivery systems have been developed for sustained subcutaneous administration of tenofovir alafenamide (TAF), a potent and effective nucleotide reverse transcriptase inhibitor used HIV pre-exposure prophylaxis (PrEP). LA platforms aim to address the lack adherence oral regimens, which has impaired PrEP efficacy. Despite extensive investigations in this field, tissue response TAF remains be elucidated as contrasting preclinical results reported...
Treatment nonadherence is a pressing issue in people living with HIV (PLWH), as they require lifelong therapy to maintain viral suppression. Poor adherence leads antiretroviral (ARV) resistance, transmission others, AIDS progression, and increased morbidity mortality. Long-acting (LA) ARV promising strategy combat the clinical drawback of user-dependent dosing. Islatravir (ISL) candidate for treatment given its long half-life high potency. Here we show constant ISL release from subdermal LA...
Abstract Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing Encapsulation (NICHE) device, which integrates direct vascularization facile mass transfer localized immunosuppressant delivery rejection prophylaxis. Here, investigated NICHE allogeneic long-term reversal in an immunocompetent rat model....
Subcutaneous delivery of cell therapy is an appealing minimally-invasive strategy for the treatment various diseases. However, subdermal site poorly vascularized making it inadequate supporting engraftment, viability, and function exogenous cells. In this study, we developed a 3D bioprinted scaffold composed alginate/gelatin (Alg/Gel) embedded with mesenchymal stem cells (MSCs) to enhance vascularization tissue ingrowth in subcutaneous microenvironment. We identified bio-ink crosslinking...
Abstract Revascularization plays a critical role in the successful engraftment of transplanted pancreatic islets, which are inherently rich capillaries to meet their high metabolic demands. Innovative islet encapsulation strategies such as NICHE (neovascularized implantable cell homing and encapsulation), generate prevascularized transplantation site that allows for direct integration graft with systemic circulation. Timing is key maximizing survival, especially diabetic individuals, who...
Abstract The impact of pre-exposure prophylaxis (PrEP) on slowing the global human immunodeficiency virus (HIV) epidemic hinges effective drugs and delivery platforms. Oral regimens have represented pillar HIV PrEP for years. However, variable adherence has spurred development long-acting systems, which also aim at increasing access, uptake persistence. Here we present an ultra-long-acting transcutaneously refillable subcutaneous nanofluidic implant constant sustained release islatravir...
In article number 2000670 by Alessandro Grattoni and co-workers, vascularization of a subcutaneously implantable cell encapsulation platform is enhanced via enrichment with plateletrich plasma (PRP) mesenchymal stem cells (MSCs) in rats non-human primates. Timepoint vascularity assessment demonstrates PRP MSCs increases blood vessel density maturity inside the devices, unveiling an optimal window for transplantation. Interspecies reproducibility points toward translatability technology.