A5083933274- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Electrospun Nanofibers in Biomedical Applications
- Immune Response and Inflammation
- HIV-related health complications and treatments
- Diabetes and associated disorders
- Advanced Drug Delivery Systems
- Atherosclerosis and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Diabetes Management and Research
- Child Abuse and Trauma
- Immunotherapy and Immune Responses
- Youth Substance Use and School Attendance
- Primate Behavior and Ecology
- Animal Genetics and Reproduction
- Metal-Catalyzed Oxygenation Mechanisms
- Stress Responses and Cortisol
- Systemic Lupus Erythematosus Research
- Cholesterol and Lipid Metabolism
- T-cell and B-cell Immunology
The University of Texas MD Anderson Cancer Center
2017-2023
Scripps MD Anderson Cancer Center
2022
Nationwide Children's Hospital
2017
Southern Methodist University
2013
Wake Forest University
2012-2013
University of Louisville
2008
HIV-1 is a chronic disease managed by strictly adhering to daily antiretroviral therapy (ART). However, not all people living with have access ART, and those may adhere treatment regimens increasing viral load progression. Here, subcutaneous nanofluidic implant was used as long-acting (LA) drug delivery platform address these issues. The device loaded tenofovir alafenamide (TAF) implanted in treatment-naïve simian HIV (SHIV)-positive nonhuman primates (NHP) for month. We monitored...
Described here is the development of gadolinium(III) texaphyrin-platinum(IV) conjugates capable overcoming platinum resistance by 1) localizing to solid tumors, 2) promoting enhanced cancer cell uptake, and 3) reactivating p53 in platinum-resistant models. Side side comparative studies these Pt(IV) clinically approved platinum(II) agents previously reported platinum(II)-texaphyrin demonstrate that present are more stable against hydrolysis nucleophilic attack. Moreover, they display high...
Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing Encapsulation (NICHE) device, which integrates direct vascularization facile mass transfer localized immunosuppressant delivery rejection prophylaxis. Here, investigated NICHE allogeneic long-term reversal in an immunocompetent, male rat model. We...
Pre-exposure prophylaxis (PrEP) using antiretroviral oral drugs is effective at preventing HIV transmission when individuals adhere to the dosing regimen. Tenofovir alafenamide (TAF) a potent drug, with numerous long-acting (LA) delivery systems under development improve PrEP adherence. However, none has undergone preventive efficacy assessment. Here we show that LA TAF novel subcutaneous nanofluidic implant (nTAF) confers partial protection from transmission. We demonstrate sustained...
The impact of pre-exposure prophylaxis (PrEP) on slowing the global HIV epidemic hinges effective drugs and delivery platforms. Oral drug regimens are pillar PrEP, but variable adherence has spurred development long-acting systems with aim increasing PrEP access, uptake, persistence. We have developed a subcutaneous nanofluidic implant that can be refilled transcutaneously for sustained release islatravir, nucleoside reverse transcriptase translocation inhibitor is used PrEP. In rhesus...
Abstract The current standard for cell encapsulation platforms is enveloping cells in semipermeable membranes that physically isolate transplanted from the host while allowing oxygen and nutrient diffusion. However, long‐term viability function of encapsulated are compromised by insufficient supply to graft. To address this need, a strategy achieve enhanced vascularization 3D‐printed, polymeric platform using platelet‐rich plasma (PRP) mesenchymal stem (MSCs) investigated. study conducted...
Nonhuman primates from domestic sources constitute a small, but critical, proportion of animals studied in research laboratories. Many these nonhuman are raised at one facility and subsequently transported/relocated to another for purposes. We examined the effects transport, relocation, acclimation on phenotype function peripheral blood mononuclear cells (PBMCs) group rhesus monkeys that were transported by road approximately 21 hours another. Using panel human antibodies set standardized...
Abstract Pre-exposure prophylaxis (PrEP) using antiretroviral oral drugs is effective at preventing HIV transmission when individuals adhere to the dosing regimen. Tenofovir alafenamide (TAF) a potent drug, with numerous long-acting (LA) delivery systems under development improve PrEP adherence. However, none has undergone preventive efficacy assessment. Here we show that LA TAF novel subcutaneous nanofluidic implant (nTAF) confers partial protection from transmission. We demonstrate...
Long-acting (LA) implantable drug delivery systems (IDDS) offer an effective approach for the management or prevention of chronic conditions by sustained parenteral therapeutic administration. LA IDDS can and improve adherence to treatment regimens minimizing dosing frequency. However, their clinical deployment is challenged factors such as poor loading capacity, which limit lifespan require repeated surgical replacement continued therapy. To address these challenges, leveraging previous...
Most human immunodeficiency virus type 1 (HIV-1) infections begin at mucosal surfaces. Providing a barrier of protection these may assist in combating the earliest events infection. Systemic immunization by intramuscular (i.m.) injection can drive immune responses, but there are data suggesting that better educate responses. To test this, rhesus macaques were immunized with replicating single-cycle adenovirus (SC-Ad) vaccines expressing clade B HIV-1 gp160 intranasal (i.n.) and i.m. routes...
NHP are a small, but critical, portion of the animals studied in research laboratories. Many imported or raised at one facility and subsequently moved to another for purposes. To improve our understanding effects transportation relocation on immune system, minimize potential confounds associated with relocation, maximize study validity, we examined phenotype function PBMC cynomolgus macaques ( Macaca fascicularis ) that were transported approximately 200 miles by road from another. We...
Several implantable long-acting (LA) delivery systems have been developed for sustained subcutaneous administration of tenofovir alafenamide (TAF), a potent and effective nucleotide reverse transcriptase inhibitor used HIV pre-exposure prophylaxis (PrEP). LA platforms aim to address the lack adherence oral regimens, which has impaired PrEP efficacy. Despite extensive investigations in this field, tissue response TAF remains be elucidated as contrasting preclinical results reported...
Treatment nonadherence is a pressing issue in people living with HIV (PLWH), as they require lifelong therapy to maintain viral suppression. Poor adherence leads antiretroviral (ARV) resistance, transmission others, AIDS progression, and increased morbidity mortality. Long-acting (LA) ARV promising strategy combat the clinical drawback of user-dependent dosing. Islatravir (ISL) candidate for treatment given its long half-life high potency. Here we show constant ISL release from subdermal LA...
Abstract Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing Encapsulation (NICHE) device, which integrates direct vascularization facile mass transfer localized immunosuppressant delivery rejection prophylaxis. Here, investigated NICHE allogeneic long-term reversal in an immunocompetent rat model....
Mitral valve construction using extracellular matrix (ECM) is a relatively new procedure. In this case, 15-month-old boy with history of severe mitral regurgitation secondary to endocarditis underwent surgery. repair was not possible, and thus, 17 mm cylinder (ECM-CV) constructed for replacement. The ECM-CV clearly imaged echocardiography, especially three-dimensional imaging, that helped define function. As the use ECM increases, echocardiography will play an essential role in evaluating...
OBJECTIVE: Assisted reproductive technology (ART) centers interested in offering investigational fertility preservation services may find start-up barriers prohibitive.In addition to the extra demands placed on physician and laboratory resources, involved be perceived as being too complicated.In vitro maturation of germinal vessicle oocytes retrieved from whole ovary specimens a good entry point into experimental protocols.Here we report development simple system preserve for women who are...
Abstract The impact of pre-exposure prophylaxis (PrEP) on slowing the global human immunodeficiency virus (HIV) epidemic hinges effective drugs and delivery platforms. Oral regimens have represented pillar HIV PrEP for years. However, variable adherence has spurred development long-acting systems, which also aim at increasing access, uptake persistence. Here we present an ultra-long-acting transcutaneously refillable subcutaneous nanofluidic implant constant sustained release islatravir...