Abstract Aims The relationship between the incidence of major cardiovascular diseases (CVDs) and estimated glucose disposal rate (eGDR), a proxy measurement for insulin resistance (IR), is not well understood in general population. predictive value eGDR other proxies IR CVD incidents have been examined previous studies. This study aimed to investigate association various events, including myocardial infarction (MI), heart failure (HF), atrial fibrillation (AF) ischemic stroke. Additionally,...

MicroRNAs (miRNAs) play key roles in many serious diseases, such as cancer. As a consequence, miRNAs are of great interest biomarkers clinical diagnostics. Simple, fast, selective, and sensitive detection miRNAs, however, is challenged by their short length, homogeneous sequence, susceptibility to degradation, low abundance human serum. Here, we present new strategy for highly selective miRNA based on the formation plasmonic Au@Ag nanosnowman. When triggered miRNA-21, bimetallic...

The recurrent somatic mutations in genes such as Janus kinase 2 (JAK2) lead to cytokine-independent activation of the JAK-signal transducer and activator transcription (STAT) pathway, a crucial factor development classic myeloproliferative neoplasms (cMPNs). Protein tyrosine phosphatase 1B (PTP1B) is significant regulator this while single nucleotide polymorphism (SNP) promoter methylation profiles PTP1B gene cMPN patients have largely remained unexplored. Therefore, further explore SNP...

Ebselen modulates target proteins through redox reactions with selenocysteine/cysteine residues, or binding to the zinc finger domains. However, a recent contradiction in ebselen inhibition of kidney type glutaminase (KGA) stimulated our interest investigating its mechanism glutamate dehydrogenase (GDH), KGA, thioredoxin reductase (TrxR), and glutathione S-transferase. Fluorescein- biotin-labeled derivatives were synthesized for mechanistic analyses. Biomolecular interaction analyses showed...

Qi S‐T, Zhou J, Pan Zhang C, Silky C & Yan X‐R (2012) Histopathology 61 , 711–725 Epithelial–mesenchymal transition and clinicopathological correlation in craniopharyngioma Aims: To assess the immunophenotypic changes associated with epithelial–mesenchymal (EMT) craniopharyngioma, especially at tumour invasive front, to correlate findings features patient outcomes. Methods results: Forty‐two craniopharyngiomas were investigated for presence of EMT markers (vimentin, E‐cadherin β‐catenin)...

There is an increased interest in extending surgical criteria for pancreatic cancer by performing pancreaticoduodenectomy (PD) combined with portal vein (PV) or superior mesenteric (SMV) resection and reconstruction borderline resectable patients. However, whether this procedure suitable elderly patients remains unclear. Here, we studied cases of treatment our medical center to evaluate feasibility safety the elderly. Eighty-three 65 years age older who underwent PD from January 2009 March...

In hepatolithiasis, chronic proliferative cholangitis (CPC), an active and longstanding inflammation of stone‑containing bile ducts with enhanced mucin‑producing activity, not only affects the progression disease, it can also induce biliary carcinogenesis. The present study aimed to examine effect epidermal growth factor receptor (EGFR) monoclonal antibody panitumumab (Pani) on CPC. Following establishment CPC rat models, periodic acid Schiff staining was used observe positive rate EGFR...

To investigate the effect of ischemic precondition to protect ischemia-reperfusion injury and reduce IL-6 expression in rats liver transplantation.The rat portal vein infusion autologous transplantation model were used. The divided into preconditioning group (A group), (B group) normal control (C group). Then we analyzed changes function, microstructure IL-6, SOD MDA within 48 h.The pathology A showed lobular architecture essentially normal, cells was slightly swell no significant...

Metabolomics studies can quantitatively detect the dynamic metabolic response of living systems. To urinary metabolomics after hepatic ischemia/reperfusion (I/R) injury induced by Pringle maneuver using gas chromatography-mass spectrometry (GC-MS). Male Sprague-Dawley rats (N = 80) were randomly divided into 4 groups (n 20/group): sham operation, day 1, 3, and 5. Rats in 5 underwent maneuver. Serum alanine transaminase (ALT) total bilirubin (TBIL) measured, hematoxylin eosin (HE) staining...

The aim of the current study was to examine expression angiotensin II type 1 receptor‑associated protein (ATRAP) in rat hepatic stellate cell line HSC‑T6 and determine its interactions with local renin‑angiotensin system (RAS). To achieve this goal, effect stimulating cells (AngII) angiotensin‑(1‑7) [Ang‑(1‑7)], on ATRAP, I receptor (AT1R), Mas converting enzyme 2 (ACE2) 2, 6, 12, 18, 24 36 h after stimulation investigated. Changes were quantified at gene level using RT‑qPCR western...

Heparin-binding epidermal growth factor-like factor (HB-EGF) is a potent mitogen and chemotactic factor. HB-EGF attenuates intestinal ischemia/reperfusion injury caused by superior mesenteric artery occlusion. We examined whether offers protection against congestion/reperfusion (C/R) injury, which portal triad clamping. Male Sprague–Dawley rats were randomly divided into three equally sized groups: I, sham-operated; II, clamping (Pringle maneuver); III, II + intraluminal administration of...

<title>Abstract</title> Renal senescence plays a crucial role in the development of diabetic kidney disease (DKD). The progression DKD can accelerate process kidney, making it more susceptible to diabetes-induced damage and accelerating renal fibrosis. However, there is no effective treatment for this pathological process. Hederagenin (Hed) has shown promising improvements fibrosis chronic DKD, but its mechanism DKD-induced remains unclear. In study, we found that Hed exhibited significant...

Objectives To evaluate the predictive value of revised model for end-stage liver disease in clinical early stage after transplantation.Methods The data 218 patients were retrospectively analyzed.After calculating MELD score,ReFit score and ReFit MELDNa before transplantation,we compared accuracies these scoring systems using area under curve (AUC) receiver operating characteristic.The groups categorized with cut-offs MELD,ReFit MELDNa,and early-stage complications mortality different...

：Objective Tocomparatively study the ischemia-reperfusion injuries caused by heterogeneity of differentpositions biliary system and different construction patterns peribiliaryvascular plexus. Methods Thirty rats were randomly divided into 3 groups: Group Ⅰ , sham operated; Ⅱ, 1h ischemia inbiliary tract followed reperfusion; Ⅲ, in biliarytract 2h reperfusion. TUNEL assay, pathomorphology score determination andultrastructural quantitative analysis performed on epithelium hilar bile...

Objective To establish a M1/M2-polarized macrophages intrahepatic transplantation model in mice. Methods Bone marrow-derived (BMDMs) were harvested. After carboxyfluorescein (FAM)-labeled small interfering RNA (siRNA) transfection, the transfected BMDM incubated to induce M1 and M2 polarization, respectively. The not induced marked M0. Then 8×105 polarized transfused mouse liver through portal vein injection. injected phosphate buffer solution (PBS) assigned control group. FAM...