A5008849859- 3D Printing in Biomedical Research
- Osteoarthritis Treatment and Mechanisms
- dental development and anomalies
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- TGF-β signaling in diseases
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Chemical Safety and Risk Management
- Neuroendocrine regulation and behavior
- Fibroblast Growth Factor Research
- Hair Growth and Disorders
- Biomedical and Chemical Research
- Inflammatory mediators and NSAID effects
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Ion Channels and Receptors
- Scientific Measurement and Uncertainty Evaluation
- melanin and skin pigmentation
- Neurotransmitter Receptor Influence on Behavior
- T-cell and B-cell Immunology
- MicroRNA in disease regulation
- Biochemical Analysis and Sensing Techniques
- Oral and Maxillofacial Pathology
- Immunotherapy and Immune Responses
Technische Universität Berlin
2007-2025
Charité - Universitätsmedizin Berlin
2007-2025
University of Lübeck
2018
German Rheumatism Research Centre
2001-2005
Multipotent haematopoietic stem and progenitor cells (HSPCs) are the source for all blood cell types. The bone marrow niche in which HSPCs maintained is known to be vital their maintenance. Unfortunately, date, no vitro model exists that accurately mimics aspects of simultaneously allows long-term culture HSPCs. In this study, a novel three-dimensional coculture presented, based on hydroxyapatite coated zirconium oxide scaffold, comprising human mesenchymal stromal (MSCs) cord derived HSPCs,...
ABSTRACT Contrary to short‐lived plasma cells, which survive only 3–5 days, long‐lived cells (LLPCs) contribute the humoral memory of body and thus also many antibody‐related diseases. The ability persist over months, years, even a lifetime has been demonstrated in vivo. Yet, vitro culture human primary bone marrow‐derived limited few days. Here, we establish conditions for 21 Plasma stromal are isolated from marrow cultured 2D or 3D ceramic scaffold. cells’ survival antibody secretion...
Abstract Functional in vitro models emulating the physiological processes of human organ formation are invaluable for future research and development regenerative therapies. Here, a developmentally inspired approach is pursued to reproduce fundamental steps tooth organogenesis using dental pulp cells. Similar vivo situation initiating mesenchymal condensation, 3D self-organizing culture was resulting an organoid size germ with odontogenic marker expression. Furthermore, model capable...
Trace amine-associated receptors (TAARs) belong to the class A G-protein-coupled (GPCR) and are evolutionary related aminergic receptors. TAARs have been identified mediate effects of trace amines. TAAR1 signaling is mainly mediated via activation Gs/adenylyl cyclase pathway. In addition classical amines, can also be activated by thyroid hormone derivative 3-iodothyronamine (3-T1AM). Pharmacological doses 3-T1AM induced metabolic anapyrexic effects, which might centrally in hypothalamus...
Abstract: A promising strategy for the regeneration of degenerated cartilage tissue structure in osteoarthritic joints is use mesenchymal precursor cells. These cells can be triggered to undergo differentiation into functional active chondrocytes resulting newly synthesized cartilage. Because chondrogenic initiated by step condensation vitro, it great interest fully characterize first lineage specific vitro . Therefore, a modified culture system was developed which mimics process and may...
3-Iodothyronamine (3-T1AM) is an endogenous thyroid hormone metabolite. The profound pharmacological effects of 3-T1AM on energy metabolism and thermal homeostasis have raised interest to elucidate its signaling properties in tissues that pertain metabolic regulation thermogenesis. Previous studies identified G protein-coupled receptors (GPCRs) transient receptor potential channels (TRPs) as targets different cell types. These two superfamilies membrane proteins are largely expressed tissue...
Abstract: Hair follicle cycling is driven by epithelial–mesenchymal interactions (EMI), which require extracellular matrix (ECM) modifications to control the crosstalk between key epithelial- and mesenchymal-derived growth factors cytokines. The exact roles of these ECM in hair cycle-associated EMI are still unknown. Here, we used differential microarray analysis laser capture-microdissected human scalp follicles (HF) identify new components that distinguish fibroblasts from connective...
Misexpression of growth factors, particularly those related to stem cell-like phenotype, is often observed in several cancer types. It has been found influence parameters disease progression like cell proliferation, differentiation, maintenance undifferentiated phenotype and modulation the immune system. GDF3 a TGFB family member associated with pluripotency differentiation during embryonic development that previously reported be re-expressed number However, its role tumor not clarified yet....
The class II transactivator is a major transcriptional factor acting on the promoters of MHC genes. Transcription CIITA gene driven by four alternative promoters, which exhibit cell-type-specific activity. promoter III (PIII) constitutively active in B cells, whereas IV (PIV) becomes activated upon interferon-gamma activation. aim this study was to investigate whether these two sequence variability like do. We isolated PIII and PIV fragments from healthy individuals rheumatoid arthritis...
Chondrocytes, comparable to many cells from the connective tissue, dedifferentiate and end up in a similar fibroblastoid cell type, marked by loss of specific expression pattern. Here, chondrocytes isolated osteoarthritic (OA) patients were investigated. The OA used this work not affected gene upon culture. mRNA levels known cartilage markers, such as SOX5, SOX6, SOX9, aggrecan proteoglycan 4, remained unchanged. Since healthy tissue show different DNA methylation patterns, underlying...
Abstract Multipotent hematopoietic stem and progenitor cells HSPC reside in specialized cell niches within the bone marrow, that provide a suitable microenvironment for lifelong maintenance of cells. Meaningful vitro models recapitulating vivo niche biology can be employed both basic research as well applied sciences represent powerful tool to reduce animal tests preclinical studies. Recently we published generation an marrow model, capable long-term cultivation HSC based on organ-on-a-chip...
Abstract Background Given regenerative therapies, the utilization of primary human cells is desired and requested in development vitro systems disease models. After a few passages , all from connective tissue end up similar fibroblastoid cell type marked by loss specific expression pattern. It still under discussion whether different de-differentiated mesenchymal have or identical differentiation capacities . Methods Chondrocytes isolated patients with late-stage osteoarthritis were cultured...