Background During the 2023-dengue outbreak in Bangladesh, a diagnostic evaluation study was conducted to investigate concurrent Zika virus (ZIKV) and dengue (DENV) transmission Dhaka 2023. Aims The explored simultaneously detect presence of ZIKV, DENV, and/or CHIKV while considering relevant clinical epidemiological risk factors, using real-time multiplex RT-PCR system. Following this, it planned sequence selected samples identify genetic variations ZIKV infections within population. Methods...

ABSTRACT. Bangladesh experienced the largest and deadliest dengue outbreak in 2023, after virus had reappeared country 2 decades earlier. A total of 1,705 people died Bangladesh, representing highest case fatality rate (0.5%) due to world for that year. The severity infection is some extent related emergence new circulating serotypes. To identify possible predominant serotype reverse transcription polymerase chain reaction-based identification technique was used on stored serum samples...

We report a case of Primaquine (PQ) induced hemoglobinuria in patient with the glucose-6-phosphate dehydrogenase (G6PD) Mahidol variant from Bandarban, Bangladesh. The presented mixed Plasmodium falciparum and vivax malaria was recommended to be treated according national guidelines Artemether-Lumefantrine for three days PQ 14 days. Ten later, developed fever jaundice, followed by twelve after initial diagnosis. This highlighted need G6PD testing, which subsequently confirmed both...

Background Malaria remains a major public health challenge, particularly in endemic regions like Bangladesh. To combat this, the National Elimination Programme (NMEP) has been working to ensure long-lasting insecticidal nets (LLINs) reach vulnerable populations. This study assessed LLIN coverage, access, and utilization among Bangladeshi population Forcibly Displaced Myanmar Nationals (FDMN). Methods A cross-sectional survey was conducted from May October 2023 across five malaria-endemic...

A single‐transmembrane protein uroplakin III (UPIII) and its tetraspanin binding‐partner Ib (UPIb) are members of the UP proteins that were originally identified in mammalian urothelium. In Xenopus laevis eggs, these proteins: xUPIII xUPIb, components cholesterol‐enriched membrane microdomains or “rafts” involved sperm–egg interaction subsequent egg activation signaling via Src tyrosine kinase at fertilization. Here, we investigate whether xUPIII‐xUPIb complex is close proximity to CD9, a...

In Xenopus laevis, sperm-egg interaction promotes partial proteolysis and/or tyrosine phosphorylation of uroplakin III (UPIII) and the kinase Src, which both localize to cholesterol-enriched egg membrane microdomains (MDs). Here we show that sperm promote UPIII Src in MDs isolated from ovulated unfertilized eggs (UF-MDs). An antibody against extracellular domain interferes with these events. Inhibition fertilization by anti-UPIII is rescued co-incubation UF-MDs. This suggests that, like...

Thalassemia and other structural haemoglobinopathies are the major erythrocyte formation disorder prevalent in certain parts of world including Bangladesh. We investigated 600 cases anaemic patients referred from various country for diagnosis counselling during 3 months (April to June 2011) time. The most common form haemoglobin (Hb) observed subjects studied was β-thalassemia minor (21.3%). Two conditions, such as E-β-Thalassemia HbE trait, were also fairly (13.5 12.1%, resp.) total...

Ensuring adherence to antimalarial treatment is crucial for achieving a radical cure and elimination of malaria, especially in hard-to-reach areas. We conducted this study assess the current scenario drug four endemic sub-districts Bangladesh. Among 110 enrolled participants, 70% were mono-infected with Plasmodium falciparum remaining 30% P. vivax. The overall frequency was 92.7% (95% CI: 83.0–96.3%). A total eight participants found be nonadherent all them from Bandarban. Level nonadherence...

In Bangladesh and the Indian subcontinent, agriculture is backbone of any country. It crucial for a nation's overall economic development. Among many social advantages that it meets people's requirements food, shelter, employment. Most our farmers use traditional farming practices. Agriculture-related projects on large scale are now occurring in Despite using advanced technology, they do not manage maintain smartly. If we handle farm management smartly, might be able to reduce lot waste....

Zinc transporter ZnT8, encoded by SLC30A8, is expressed highly in pancreatic β-cells that effluxes Zn2+ into insulin granules which required to secret from the granules. Genome-wide association study identified twelve loss of function mutations SLC30A8 play protective role against type 2 diabetes (T2D). This aimed find genetic a protein truncating variant rs200185429 Bangladeshi healthy individuals (n = 184), patients with prediabetes 130) and T2D 179). Genetic respect was performed using...

ABSTRACT Toxigenic Vibrio cholerae strains are lysogens of CTXΦ, a filamentous bacteriophage which encodes cholera toxin (CT). Following infection recipient V. cells by the phage genome either integrates into host chromosome at specific attachment site ( att RS) or exists as replicative-form (RF) plasmid. We infected naturally occurring RS-negative nontoxigenic attenuated (CTX − RS negative) derivatives wild-type toxigenic with CTXΦ and examined diarrheagenic potential carrying RF using...

Prevalence of toxigenic Vibrio cholerae O1 in aquatic reservoirs Bangladesh apparently increases coinciding with the occurrence seasonal cholera epidemics. In between epidemics, these bacteria persist water mostly as dormant cells, known viable but non-culturable cells (VBNC), or conditionally environmental (CVEC), that fail to grow routine culture. CVEC resuscitate active when enriched culture medium supplemented quorum sensing autoinducers CAI-1 AI-2 which are signal molecules regulate...

A tyrosine-phosphorylated protein of 33 kDa is shown to be present in the solubilized yolk fraction Xenopus laevis oocytes, eggs, and early embryos. The phosphoprotein lipovitellin 2 as demonstrated by immunoprecipitation mass spectrometry studies, termed pp33/LV2. Sub-cellular fractionation immunoblotting studies demonstrate that pp33/LV2 stably Triton X-100-resistant SDS-soluble fractions during oogenesis, oocyte maturation, embryogenesis. From after swimming tadpole stages (stage 39∼),...

Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now considered as the major driving force for conversion non-pathogenic to pathogenic ones. Evolution highly antibiotic largely depends upon horizontal gene transfer by means plasmid, megaplasmid bacteriophages. Conversely, may acquire novel defence mechanism called CRISPR...

<title>Abstract</title> <bold>Background</bold> Among 13 endemic districts, the Chittagong Hill Tracts bear more than 90% of Bangladesh's malaria burden. Despite private sector's prominence in rural healthcare, its role management remains underutilized. This study aimed to strategize leveraging for-profit sector, such as diagnostic and treatment centers, bolster national surveillance control, advancing Bangladesh toward elimination by 2030. <bold>Methods</bold> mixed-method commenced with a...

Abstract Background During the 2023-dengue outbreak in Bangladesh, a diagnostic evaluation study was conducted to investigate concurrent Zika virus (ZIKV) and dengue (DENV) transmission Dhaka 2023. Aims The explored simultaneously detect presence of ZIKV, DENV, and/or CHIKV while considering relevant clinical epidemiological risk factors, using real-time multiplex RT-PCR system. Following this, it planned sequence selected samples identify genetic variations ZIKV infections within...

<title>Abstract</title> <bold>Background</bold>: Dengue viruses have been prevalent since 2000 in Bangladesh, including Chattogram. The objective of the present study was to examine serotype patterns dengue 2022 and 2023 Chattogram, southern part Bangladesh. <bold>Methods: </bold>Present conducted Chattogram Maa O Shishu Hospital from June through December, October December 2023. Patients clinically suggestive fever blood NS1 test positive, were recruited study. A total 80 (2022 - 68 cases...

Among 13 endemic districts, the Chittagong Hill Tracts bear more than 90% of Bangladesh's malaria burden. Despite private sector's prominence in rural healthcare, its role management remains underutilized. This study aimed to strategize leveraging for-profit sector, such as diagnostic and treatment centers, bolster national surveillance control, advancing Bangladesh toward elimination by 2030.

Chikungunya, an arboviral disease transmitted by Aedes mosquitoes, shares clinical similarities with dengue, but is distinguished prolonged joint pain. Following a major outbreak in Bangladesh 2017, chikungunya nearly vanished from the region. This diagnostic evaluation study prospectively recruited participants meeting specific inclusion criteria and obtained written informed consent. Out of 1280 febrile individuals screened, 569 met fever onset within 2-5 days, accompanied symptoms such as...

This cross-sectional research was managed for the deduction of nutritional condition among adolescent boys a perceived charitable orphanage in Dhaka city, Bangladesh. The sample size study 100. set up mean age as 9.93 ± 2.392 years and modes Body Mass Index (BMI) 14.48 2.385. Among them 60% participants were illiterate 40% had primary level education. Of participants, 100% laid hold vegetables rice. them, 92%, 89%, 64%, 47%, 4% taken chicken, fast food, beef, bread, mutton respectively. 96%...

Abstract The study aimed to evaluat Bacillus subtilis biodegrdation one of organic-pesticides in different periods. was isolated from Iraqi soils, identification by morphological and biochemical tests. best growth B. were in(72hours) / (10-15) ppm (0.200-0.196)respectively, while the 5 showed highest 60 day (0.163). degradation rate% for 15 ppm/14 days (90.32)%. evluation (N-phosphonomethyl)glycine residues concentration’s via HPLC ratio%, with increasing time incubation 30 days, ratio%...

Background: Although the global thalassemia zone covers Bangladesh, there are very limited studies conducted in this region. Therefore, focus of our study is to understand prevalence and burden hemoglobinopathy Bangladesh. Methods: The analysis was based on a retrospective evaluation laboratory diagnoses between 2007 January 2021 October. A total 8503 specimens were sampled analyzed which either referred by corresponding physicians or self-referred. This neither any epidemiological...