A5102816405- Neuroscience and Neuropharmacology Research
- Retinal Development and Disorders
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neural Engineering
- Nicotinic Acetylcholine Receptors Study
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Gene expression and cancer classification
- Neurotransmitter Receptor Influence on Behavior
- RNA Interference and Gene Delivery
- Ion channel regulation and function
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Conducting polymers and applications
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Neurobiology and Insect Physiology Research
- Autism Spectrum Disorder Research
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
Hainan University
2025
Massachusetts Institute of Technology
2011-2020
Institute of Cognitive and Brain Sciences
2013-2020
Allen Institute for Brain Science
2020
Brown University
2020
Stanford University
2006-2015
Howard Hughes Medical Institute
2015
Georgetown University
2011
Palo Alto University
2006
Although brain-derived neurotrophic factor (BDNF) is known to regulate circuit development and synaptic plasticity, its exact role in neuronal network activity remains elusive. Using mutant mice (TrkB-PV −/− ) which the gene for BDNF receptor, tyrosine kinase B receptor ( trkB ), has been specifically deleted parvalbumin-expressing, fast-spiking GABAergic (PV+) interneurons, we show that TrkB structurally functionally important integrity of hippocampal network. The amplitude glutamatergic...
Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disability that demonstrates impaired social interactions, communication deficits, and restrictive repetitive behaviors. ASD has strong genetic basis many ASD-associated genes have been discovered thus far. Our previous work shown loss of expression the X-linked gene NEXMIF/KIDLIA implicated in patients with autistic features intellectual (ID). To further determine causal role disorder, to understand cellular molecular...
Background and Purpose Nicotinic acetylcholine receptors (nAChRs), which are expressed throughout the mammalian brain, mediate a variety of physiological functions. Despite their widespread presence, functions nAChRs not yet fully understood. α-Conotoxins, peptides derived from venom marine cone snails, target different subtypes nAChRs. Specifically, α-Conotoxins [D1G, ΔQ14] LvIC, identified Conus lividus , have demonstrated strong activity on α6β4* in vitro . However, effects LvIC been...
Nicotine addiction is a serious global public health problem, so there an urgent necessity to develop novel effective smoking cessation treatments with fewer adverse effects. Spontaneous behavioral sensitization induced by repeated intermittent exposure the addictive substance represents classical animal model of research. A significant contributor nicotine its interaction α6β2* nAChRs located on midbrain dopaminergic neurons, which leads increase in dopamine (DA) release. α-Conotoxin...
Abstract Shank proteins, one of the principal scaffolds in postsynaptic density (PSD) glutamatergic synapses, have been associated with autism spectrum disorders and neuropsychiatric diseases. However, it is not known whether different family proteins distinct functions regulating synaptic transmission, how they differ from other scaffold this aspect. Here, we investigate role Shanks transmission at rat hippocampal SC-CA1 using lentivirus-mediated knockdown molecular replacement combined...
Experience-dependent synapse refinement is essential for functional optimization of neural circuits. However, how sensory experience sculpts excitatory synaptic transmission poorly understood. Here, we show that despite substantial remodeling connectivity, AMPAR-mediated remains at equilibrium during the critical period in mouse primary visual cortex. The maintenance this requires neurogranin (Ng), a postsynaptic calmodulin-binding protein important plasticity. With normal experience, loss...
In the postsynaptic density of glutamatergic synapses, discs large (DLG)-membrane-associated guanylate kinase (MAGUK) family scaffolding proteins coordinates a multiplicity signaling pathways to maintain and regulate synaptic transmission. Postsynaptic density-93 (PSD-93) is most variable paralog in this family; it exists six different N-terminal isoforms. Probably because structural functional variability these isoforms, role PSD-93 remains controversial. To accurately characterize PSD-93,...
Significance De novo protein synthesis is critical for memory formation. We found that during acquisition transiently required contextual identified one candidate gene, Nrgn (encoding neurogranin, Ng) with enhanced translation upon novel-context exposure, and experience-dependent of Ng in the hippocampus Furthermore, fragile-X mental retardation interacts 3′UTR mRNA, which activity-dependent synaptic compartment Together, these results indicate acute enables durable context encoding.
The postsynaptic density (PSD)-95-like, disk-large (DLG) membrane-associated guanylate kinase (PSD/DLG-MAGUK) family of proteins scaffold α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) complexes to the compartment and are postulated orchestrate activity-dependent modulation synaptic AMPAR functions. SAP102 is a key member this family, present from early development, before PSD-95 PSD-93, throughout life. Here we investigate role in transmission using cell-restricted...
PSD-95-like, disc-large (DLG) family membrane-associated guanylate kinase proteins (PSD/DLG-MAGUKs) are essential for regulating synaptic AMPA receptor (AMPAR) function and activity-dependent trafficking of AMPARs. Using a molecular replacement strategy to replace endogenous PSD-95 with SAP97β, we show that the prototypic β-isoform PSD-MAGUKs, has distinct NMDA (NMDAR)-dependent roles in basic properties AMPAR-containing synapses. SAP97β enhances number synapses an NMDAR-dependent manner,...
Abstract NRGN is a schizophrenia risk gene identified in recent genetic studies, encoding small neuronal protein, neurogranin (Ng). Individuals carrying variant of showed decreased hippocampal activation during contextual fear conditioning. Furthermore, the expression Ng was reduced post-mortem brains schizophrenic patients. Using mouse model, we found that translation hippocampus rapidly increased response to novel context exposure, and this up-regulation required for memory. The extent...
Abstract Anatomical methods for determining cell-type specific connectivity are essential to inspire and constrain our understanding of neural circuit function. We developed new genetically-encoded reagents fluorescence-synapse labeling analysis in brain tissue, using a fluorogen-activating protein (FAP)-or YFP-coupled, postsynaptically-localized neuroligin-1 targeting sequence (FAP/YFPpost). Sparse viral expression FAP/YFPpost with the cell-filling, red fluorophore dTomato (dTom) enabled...
Advances in protein tagging and mass spectrometry have enabled generation of large quantitative proteome phosphoproteome data sets, for identifying differentially expressed targets case–control studies. The power study statistical tests is critical designing strategies effective target identification control experimental cost. Here, we develop a simulation framework to generate realistic phospho-peptide with known changes between cases controls. Using this framework, quantify the performance...