A5040401454- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Abdominal Surgery and Complications
- Hydrogels: synthesis, properties, applications
- Tendon Structure and Treatment
- Muscle and Compartmental Disorders
- biodegradable polymer synthesis and properties
- Cardiac, Anesthesia and Surgical Outcomes
- Orthopedic Surgery and Rehabilitation
- Tissue Engineering and Regenerative Medicine
- Lanthanide and Transition Metal Complexes
- Anatomy and Medical Technology
- Cardiovascular and Diving-Related Complications
- Mesenchymal stem cell research
- Connective tissue disorders research
- Wound Healing and Treatments
- Bee Products Chemical Analysis
- Medical Imaging Techniques and Applications
- Inorganic Fluorides and Related Compounds
- Advanced MRI Techniques and Applications
- Nerve injury and regeneration
- Polymer Surface Interaction Studies
- Sports injuries and prevention
Ghent University
2018-2024
Ghent University Hospital
2019-2024
Ablynx (Belgium)
2024
John Wiley & Sons (United States)
2022
Various biopolymers, including gelatin, have already been applied to serve a plethora of tissue engineering purposes. However, substantial concerns arisen related the safety and reproducibility these materials due their animal origin risk associated with pathogen transmission as well batch-to-batch variations. Therefore, researchers focusing attention toward recombinant that can be produced in laboratory full designed according specific needs (e.g., by introducing additional RGD sequences)....
Abstract Adipose tissue engineering aims to provide solutions patients who require reconstruction following mastectomies or other soft trauma. Mesenchymal stromal cells (MSCs) robustly differentiate into the adipogenic lineage and are attractive candidates for adipose engineering. This work investigates whether pore size modulates differentiation of MSCs toward identifying optimal scaffold spatial infiltration adipogenically differentiated cells. To assess this, extrusion‐based 3D printing...
Photocrosslinkable gelatin hydrogels are excellent bioinks or biomaterial ink components to serve biofabrication applications. Especially the widely investigated gelatin-methacroyl (gel-MA) hold an impressive track record. However, over past decade, increasing attention is being paid thiol-ene photo-click chemistry obtain hydrogel networks benefitting from a faster reactivity (i.e. seconds vs minutes) along with superior biocompatibility and processability. In order exploit this chemistry,...
Nowadays, breast implants, lipofilling, and microsurgical free tissue transfer are the most often applied procedures to repair soft defects resulting from mastectomies/lumpectomies following cancer. Due drawbacks limitations associated with these conventional clinical practices, there is a need for alternative reconstructive strategies. The development of biomimetic materials able promote cell proliferation adipogenic differentiation has gained increasing attention in context adipose...
Abstract Soft tissue engineering has been gaining increasing interest as an approach to overcome the limitations posed by current clinical procedures such invasiveness of surgery, post-operative complications and volume loss. damage occurs either due congenital malformation, trauma/disease or surgical resection. Through use autologous cells, mesenchymal stem combined with a biomaterial acting support, biological substitutes can be developed. A promising pathway in terms delivery these...
Acrylate-based photo-cross-linked poly(ε-caprolactone) (PCL) tends to show low elongation and strength. Incorporation of osteo-inductive hydroxyapatite (HAp) further enhances this effect, which limits its applicability in bone tissue engineering. To overcome this, the thiol–ene click reaction is introduced for first time order photo-cross-link PCL composites with 0, 10, 20, 30 wt % HAp nanoparticles. It demonstrated that at break ultimate strength increase 10- 2-fold, respectively, when...
Abstract Gelatin is used widely in the biomedical field, among other for wound healing. Given its upper critical solution temperature, crosslinking required. To this end, gelatin chemically modified with different photo‐crosslinkable moieties low (32–34%) and high (63–65%) degree of substitution (DS): gelatin‐methacrylamide (gel‐MA) gelatin‐acrylamide (gel‐AA) gelatin‐pentenamide (gel‐PE). Next to more researched gel‐MA, it especially interesting novel compare gelatin‐derived compounds...
The tissue engineering field is currently advancing towards minimally invasive procedures to reconstruct soft defects. In this regard, injectable hydrogels are viewed as excellent scaffold candidates support and promote the growth of encapsulated cells. Cross-linked gelatin methacryloyl (GelMA) gels have received substantial attention due their extracellular matrix-mimicking properties. particular, GelMA microgels were recently identified interesting materials since pores in between microgel...
Gelatin-based hydrogels emerged as promising biodegradable cell-compatible 3D-printable materials with tunable mechanical properties that serve tissue engineering and applications in regenerative medicine. Nevertheless, these are very challenging to monitor vivo, which has hampered the further development of their translation into clinical practice. To overcome this limitation, we designed a cross-linked gelatin-based hydrogel endowed poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA). Such...
Poly(ɛ-caprolactone) (PCL) is a biocompatible, biodegradable, and highly mechanically resilient FDA-approved material (for specific biomedical applications, e.g. as drug delivery devices, in sutures, or an adhesion barrier), rendering it promising candidate to serve bone tissue engineering. However, vivo monitoring of PCL-based implants, well biodegradable implants general, their degradation profiles pose significant challenge, hindering further development the engineering field subsequent...
(1) Background: Tendinopathy is a common injury in both human and equine athletes. Representative vitro models are mandatory to facilitate translation of fundamental research into successful clinical treatments. Natural biomaterials like gelatin provide favorable cell binding characteristics easily modifiable. In this study, methacrylated (gel-MA) norbornene-functionalized (gel-NB), crosslinked with 1,4-dithiotreitol (DTT) or thiolated (gel-SH) were compared. (2) Methods: The physicochemical...
A reinforced tubular, medicated electrospun construct was developed for deep flexor tendon repair. This combines mechanical strength with the release of anti-inflammatory and anti-adhesion drugs. In this study, evaluated using a rabbit model. It compared to its components (a polymer without reinforcement tubular braid as such) on one hand modified Kessler suture control group. Forty New Zealand rabbits were randomly divided into two groups. Surgery performed in second fourth tendons hind paw...
The potential of recombinant materials in the field adipose tissue engineering (ATE) is investigated using a bottom-up (TE) approach. This study explores synthesis different photo-crosslinkable gelatin derivatives, including both natural and materials, with particular emphasis on chain growth step polymerization. Gelatin type B (Gel-B) collagen peptide (RCPhC1) are used as starting materials. gel fraction mass swelling properties 2D hydrogel films evaluated, revealing high fractions...
Adipose tissue engineering (ATE) has been gaining increasing interest over the past decades, offering promise for new and innovative breast reconstructive strategies. Animal-derived gelatin- methacryloyl already applied in a plethora of TE However, due to clinical concerns, related potential occurrence immunoglobulin (IgE)-mediated immune responses pathogen transmission, shift towards defined, reproducible recombinant proteins occurred. In present study, protein based on human collagen type...
Motivation: Gelatin-based scaffolds may be seeded by cells or serve as depots for drugs and ensure their slow release. Goal(s): The goal of the study was to prepare with tunable properties monitor stability in time a mouse model vivo. Approach: implanted mice were monitored 1H/19F MRI, fluorine content quantified 19F MR spectroscopy. Results: can easily MRI/MRS. confirmed that design fine-tuned future applications, biodegradation rates set from several weeks up one year. Impact: pave road...