A5025555320- Monoclonal and Polyclonal Antibodies Research
- Cystic Fibrosis Research Advances
- Eicosanoids and Hypertension Pharmacology
- Adrenal Hormones and Disorders
- Microfluidic and Capillary Electrophoresis Applications
- Blood Coagulation and Thrombosis Mechanisms
- Genomics and Rare Diseases
- Neonatal Respiratory Health Research
- Pregnancy and preeclampsia studies
- Inflammatory mediators and NSAID effects
- Acute Myocardial Infarction Research
- Advanced biosensing and bioanalysis techniques
- Biomarkers in Disease Mechanisms
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Coronary Interventions and Diagnostics
- Systemic Lupus Erythematosus Research
- Diabetes and associated disorders
- Mycobacterium research and diagnosis
- Sexual Differentiation and Disorders
- Biomedical and Engineering Education
- Advanced Biosensing Techniques and Applications
- Cardiac Imaging and Diagnostics
- Cardiac electrophysiology and arrhythmias
- Urticaria and Related Conditions
Surgut State University
2019-2025
Engelhardt Institute of Molecular Biology
2005-2006
Background/Objectives: The aim of this study was to evaluate the avidity thyroid autoantibodies (Abs) in sera patients with autoimmune disease (AITD) and autoantibody carriers without diagnosed AITD. Methods: A hydrogel microarray-based multiplex assay chaotrope destruction stage developed measure disease-associated autoantibodies, including those targeting thyroperoxidase (TPO), thyroglobulin (Tg), other minor antigens. Results: Evaluation three independent cohorts patients, totaling 266...
The aim of the study was to analyze associations rs2305948 KDR (VEGFR2) genotypes, state collateral coronary blood flow, as well intensity statin therapy with development structural and functional remodeling left ventricle onset ischemic cardiovascular events during long-term observation in patients myocardial infarction (MI). Material methods . prospective observational included 51 acute MI ST-segment elevation. All underwent angiography angioplasty stenting arteries, echocardiography...
Uniparental disomy is an anomaly where both homologous chromosomes are inherited from the same parent. The pathological effects of this associated with imprinting disorders and loss heterozygosity. Traditional diagnostic methods, such as simple sequence repeats analysis chromosomal microarray analysis, have limitations in diagnosing single-parent households mosaic cases. aim study to evaluate potential effectiveness a method for uniparental based on DNA methylation profiling using...
Background: a study of the three-year modality GRACE 2.0 score, as well serum activity biomarkers myocardial stress (soluble protein suppressor tumorigenicity (ST2) and copeptin) fibrosis (metalloproteinase-9 (MMP-9) galectin-3) in individuals with acute infarction (MI) for comprehensive assessment risk major ischemic events (MIEs) is scientific interest. Aim: to evaluate associations between score cardiac reported patients MI (up 24 hours) MIEs during 4-year post-infarction follow-up....
ABSTRACT This paper compares five commercially available DNA extraction methods with respect to efficiency of Salmonella enterica serovar Enteritidis from soil, manure, and compost uses an Escherichia coli strain harboring a plasmid expressing green fluorescent protein as general internal procedural control. Inclusion this control permitted more accurate quantification amplification S. in these samples reduced the possibility false negatives. With protocol it was found that optimal method...
Abstract The optimal design of oligonucleotide microchips and efficient discrimination between perfect mismatch duplexes strongly depend on the external transport target DNA to cells with immobilized probes as well respective association dissociation rates at duplex formation. In this paper we present relevant theory for hybridization fragments in flat substrate. With minor modifications, our also is applicable reaction-diffusion kinetics surface microbeads immersed solution. main...
The development of prognostic models for the identification high-risk myocardial infarction (MI) patients is a crucial step toward personalized medicine. Genetic factors are known to be associated with an increased risk cardiovascular diseases; however, little about whether they can used predict major adverse cardiac events (MACEs) MI patients. This study aimed build machine learning (ML) model MACEs in based on clinical, imaging, laboratory, and genetic features assess influence genetics...
Abstract The efficiency of discrimination between perfect and mismatched duplexes during hybridization on microchips depends the concentrations target DNA in solution immobilized probes, buffer composition, temperature is determined by both thermodynamic relationships kinetics. In this work, optimal conditions were studied using fluorescently labeled with custom-made gel-based oligonucleotide microchips. higher concentration probes association constant, formed stronger corresponding...
Autoantibodies against type 1 interferons (IFN-I) are a highly specific marker for autoimmune polyglandular syndrome (APS-1). Moreover, determination of antibodies to omega-interferon (IFN-ω) and alpha2-interferon (IFN-α2) allows short-term diagnosis in patients with isolated atypical forms APS-1. In this study, comparison three different methods, namely multiplex microarray-based, cell-based enzyme-linked immunosorbent assays detection alpha2-interferon, was carried out. A total 206 serum...
The formation of biobanks in the structure scientific and treatment diagnostic institutions with prospects for interregional integration is a fundamental link monitoring predicting diseases various origins, creating testing highly effective diagnostics, developing novel therapeutic agents. Aim . To describe standard operating procedures principles bioresource collections (BRC) medical biobanking. Material methods data practical biomedical projects using BRC obtained from patients genetic,...
Cardiovascular diseases (CVD) are a leading cause of mortality in many countries worldwide. Genetic testing is an integral part CVD prevention. The most common hereditary the practice cardiologist cardiomyopathy and channelopathy (arrhythmogenic syndromes), as well familial hypercholesterolemia, which causes high risk atherosclerosis coronary heart disease (CHD). total carrier frequency pathogenic variants 1:100. Timely genetic diagnosis necessary for conditions that can lead to sudden...
Aim. To determine the associations of allelic variants CYP2C19 gene with coronary atherosclerosis and ischemic events in patients myocardial infarction (MI) living Khanty-Mansi Autonomous Okrug — Yugra. Material methods. This prospective observational study included 203 acute MI who underwent percutaneous intervention. Patients also genetic testing using real-time polymerase chain reaction to gene. Using biostatistical analysis methods, were established between genotypes MI, their clinical...
Autoantibodies against type 1 interferons (IFN-I) are highly specific marker for autoimmune polyglandular syndrome (APS-1). In this study, a comparison of three different me-thods, namely, multiplex microarray-based, cell-based and enzyme-linked immunosorbent assays detection antibodies omega-interferon alpha2-interferon was carried out. A total 206 serum samples from adult patients with APS-1, APS-2, isolated endocrine pa-thologies, non-autoimmune disorders healthy individuals were...
Abstract Background 21-hydroxylase deficiency is present in 90–95% of cases congenital adrenal hyperplasia (CAH). Eleven major pathogenic variants account for 93% all identified the CYP21A2 gene various clinical forms disease. Each population has its own range significant variants. We aimed to study frequency using NGS technology and real-time PCR Surgut patients with different CAH. was performed on 70 salt-wasting non-classical deficiency, verified by direct Sanger sequencing PCR–RFLP...
Successful reproduction in mammals requires gamete development, fertilization, and early embryonic development. Defects any of these processes can lead to infertility, recurrent miscarriages, congenital defects. The clinical recognition the genetic causes female reproductive insufficiency using increasingly advanced technologies poses a serious challenge for medicine 21 st century. Herein, current literature on factors involved losses was summarized. A search conducted Web Science, MEDLINE,...
Potocki-Lupski syndrome (PTLS, OMIM # 610883) is a rare genetic developmental disorder resulting from partial heterozygous microduplication at chromosome 17p11.2. The condition characterized by wide variability of clinical expression, which can make its and molecular diagnosis challenging. We report here family (mother her two children) diagnosed with PTLS. When examining children, neurological psychological (neuropsychiatric) manifestations (speech delay, mild mental retardation), motor...
Aim. To evaluate clinical associations and predictive value of lipoprotein transport metabolism markers determined in a collection serum samples from patients with myocardial infarction (MI). Material methods . Collection blood 88 the acute MI was created Biobank Yugra laboratory for subsequent biochemical assessment levels markers. All were included prospective study 48 months registration medical events remote tomographic coronary artery (CA) involvement upon follow-up completion. Results...
BACKGROUND:Recurrent pregnancy loss is a serious clinical problem that complicates about 2% of pregnancies. There evidence thrombophilia and disorders folate-methionine metabolism may cause recurrent loss.
 AIM:The aim this study was to evaluate the contribution polymorphic variants genes hemostasis system cycle in women with MATERIALS AND METHODS:Clinical examination 406 pregnant divided into two groups carried out. The main group consisted 206 or more losses known up 12 weeks...
Aim. Creation of a sample collection whole blood, leukocyte suspension, genomic deoxyribonucleic acid from patients with myocardial infarction for fundamental and applied research in medicine. Materials methods . The collection, transportation processing biomaterial samples the biobank Medical Institute Surgut State University were carried out according to standard using recommendations, operating procedures national guidelines biobanking. Results. As September 1, 2023, has collected 744...
A male factor, commonly associated with poor semen quality, is revealed in about 50% of infertile couples. CFTR gene (Cystic Fibrosis Transmembrane Conduction Regulator) variants are one the common genetic causes azoospermia-related infertility. Notably, spectrum and frequency pathogenic vary between populations geographical regions. In this work, we made an attempt to evaluate allele (AF) 12 Russian men healthy individuals from different districts Russia. Because limited number...
Hypothesis/aims of study. The search for early predictors preeclampsia currently remains relevant. There is still a need to study maternal factors affecting the development such as intergenic interactions in pregnant woman with single nucleotide polymorphisms (SNPs) genes associated hemostasis system and folate cycle, well predictors. aim this was assess role comorbid pathology gene polymorphism cycle predicting woman.
 Study design, materials methods. We examined 158 women two groups,...