Manoel Sebastião da Costa Lima

ORCID: 0000-0003-0146-7123
Publications
Citations
Views
---
Saved
---
About
Contact & Profiles
Research Areas
  • Research on Leishmaniasis Studies
  • Trypanosoma species research and implications
  • Leptospirosis research and findings
  • Parasites and Host Interactions
  • Insect and Pesticide Research
  • T-cell and Retrovirus Studies
  • Plant Pathogens and Fungal Diseases
  • Fungal Infections and Studies
  • Mosquito-borne diseases and control
  • Radiation Effects and Dosimetry
  • Herpesvirus Infections and Treatments
  • Child Nutrition and Water Access
  • Child and Adolescent Health
  • Insect symbiosis and bacterial influences
  • History of Medicine and Tropical Health
  • Infectious Diseases and Mycology
  • COVID-19 Impact on Reproduction
  • Vector-Borne Animal Diseases
  • Insect Pest Control Strategies
  • Women's cancer prevention and management
  • Soil Carbon and Nitrogen Dynamics
  • Yeasts and Rust Fungi Studies
  • Vaccine Coverage and Hesitancy
  • Syphilis Diagnosis and Treatment
  • Agricultural and Food Sciences

Fundação Oswaldo Cruz
2016-2024

Universidade Federal de Mato Grosso do Sul
2011-2016

Instituto Federal de Educação, Ciência e Tecnologia de Mato Grosso
2013

Leishmaniasis is a "neglected tropical disease" and serious public health issue in Brazil. While dogs are recognized as particularly important reservoirs, recent reports of domestic cats infected with Leishmania sp. urban areas suggest their participation the epidemiological chain parasite endemic areas. The aim this study was to screen for infection an area where human canine visceral leishmaniasis endemic, followed by identification species circulating cats. We collected peripheral blood,...

10.1016/j.actatropica.2016.11.013 article EN publisher-specific-oa Acta Tropica 2016-11-13

Objective: This study sought to evaluate soil microbial activity in an agroforestry system order understand its importance for maintaining fertility. Theoretical Reference: Soil can act as a carbon reservoir, reducing the concentration of CO₂ atmosphere. The greater quantity microorganisms present this environment, better microbiological characteristics development. Therefore, systems represent effective environment capturing carbon, acting CO2 sinks and contributing climate change...

10.24857/rgsa.v19n4-053 article EN Revista de Gestão Social e Ambiental 2025-04-09

Transfusion-transmitted leishmaniasis has been a concern in regions endemic for the disease. Whether immediate or delayed, risks posed by this mode of transmission call careful assessment. The purpose study was to detect Leishmania infection blood donors living an area and investigate progression disease these individuals. Immunofluorescent antibody test, enzyme-linked immunosorbent assay, rapid polymerase chain reaction were applied 430 initial evaluation. Of those with at least one...

10.1371/journal.pone.0198199 article EN cc-by PLoS ONE 2018-06-14

Background Visceral leishmaniasis (VL) in HIV-positive individuals is a global health problem. HIV- Leishmania coinfection worsens prognosis and mortality risk, coinfected are more susceptible to VL relapses. Early initiation of antiretroviral therapy can protect against infection living VL-endemic areas, regular use antiretrovirals might prevent relapses these individuals. We conducted cross-sectional study Petrolina, Brazil, an area, estimate the prevalence asymptomatic cases among...

10.1371/journal.pntd.0009067 article EN cc-by PLoS neglected tropical diseases 2021-01-21

This study has estimated the risk of Leishmania transmission via blood transfusion in one largest banks Northeastern Brazil, where visceral leishmaniasis is endemic. Five hundred samples from donors were tested for circulating spp. DNA by real-time PCR. Positive a species-specific conventional PCR targeting infantum . Overall, 6.2% (95% CI: 4.1–8.3%) carried and sample species was confirmed as L. No statistically significant differences found relation to gender, sex, education level,...

10.1590/s1678-9946202062092 article EN cc-by-nc Revista do Instituto de Medicina Tropical de São Paulo 2020-01-01

Visceral leishmaniasis has been reported in all Brazilian regions and, Mato Grosso do Sul State, the occurrence of cases increased significantly. The objective this study was to identify natural infection phlebotomines by Leishmania Ponta Porã, a county bordering Paraguay. By using light CDC and Shannon traps, 185 sandfly females were captured, dissected arranged 107 pools subjected DNA extraction amplification Polymerase chain reaction. From samples amplification, fragment 120 bp...

10.1016/s2222-1808(11)60060-1 article EN Asian Pacific Journal of Tropical Disease 2011-12-01

Leishmania infantum is the etiological agent of visceral leishmaniasis (VL) in Americas with domestic dogs being its major reservoir hosts. The main VL vector sandfly Lutzomyia longipalpis , while other species may play a role disease transmission. Although genetic structure L. populations has been widely evaluated, only few studies have addressed this subject coupled to respective vectors. In study, we analyzed population three endemic areas Brazil and associated it geographic structure.

10.1155/2016/9249217 article EN cc-by BioMed Research International 2016-01-01

Peripheral blood of 400 dogs infected with Leishmania and Ehrlichia were analyzed using polymerase chain reaction (PCR), clinical signs characterized.PCR parasitological tests conducted.PCR was positive for in 84.75%, showed that 63.25% 31.75% Ehrlichia, respectively. All animals more than three signs. PCR results negative 15.25% the samples.Conventional peripheral can be used diagnosing canine visceral leishmaniasis combination other techniques, especially uncertain cases need species...

10.1590/0037-8682-0040-2018 article EN cc-by Revista da Sociedade Brasileira de Medicina Tropical 2018-12-01

Objective: To determine an algorithm for molecular diagnosis of visceral leishmaniasis (VL) by kinetoplast DNA (kDNA) (RV1/ RV2) and internal transcriber spacer (ITS1) (LITSR/L5.8S) polymerase chain reaction (PCR), complemented ITS1 PCR restriction fragment length polymorphism (RFLP), using peripheral blood or bone marrow aspirate from patients with suspected VL.Methods: Biological samples were submitted to the gold standard VL represented PCR, kDNA RFLP. The obtained seven groups: group I,...

10.4103/1995-7645.275414 article EN Asian Pacific Journal of Tropical Medicine 2020-01-01

Visceral leishmaniasis (VL) is a severe chronic disease caused by Leishmania (Leishmania) infantum chagasi. Better knowledge on the effects this can help develop adequate clinical management and treatment. Parasitological immunohistochemical studies were performed golden hamsters Mesocricetus auratus infected with bone marrow from individuals VL in State of Mato Grosso do Sul, central-west Brazil. The parasitism spleen, liver, kidneys, lungs, heart brain animals examined. Eighteen inoculated...

10.1590/s1984-29612011000200002 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2011-06-01

Abstract Studies of the geographic distribution sand flies and factors associated with their occurrence are necessary to understand risk leishmaniasis transmission. The objective this study was characterize fly fauna, particularly spatial Lutzomyia longipalpis (Lutz & Neiva), correlate these climate in Dourados municipality, Brazil. collection carried out CDC Light Traps over two periods: at six sites for three consecutive nights each month from August 2012 July 2013; four other April...

10.1093/jme/tjab022 article EN Journal of Medical Entomology 2021-03-16

Abstract The dog is the main domestic reservoir of Leishmania and font infection for vector, constituting an important host transmission parasite to humans. Non-invasive collection swab samples leishmaniasis diagnosis has been a promising alternative. This study analyzed positivity polymerase chain reaction (PCR) canine in conjunctiva samples. DNA extraction was performed using SDS 20% PCR 13A/13B primers that amplify 120-bp kDNA. Of 77 dogs analyzed, 50 (64.93%) had ocular changes: 25...

10.1590/s1984-29612021061 article EN cc-by Revista Brasileira de Parasitologia Veterinária/Brazilian Journal of Veterinary Parasitology 2021-01-01

This article contains the data regarding Leishmania species identification in human and canine clinical samples from a Brazilian region endemic for (Viannia) spp., (Leishmania) infantum amazonensis, using previously developed approach involving two qPCR assays (qPCR-ML qPCR-ama). The are related to "Real-time PCR differentiate among subgenus, amazonensis: application on samples" [1], include also details of evaluation/diagnosis patients primer sequences used assays. has been determined 27 11...

10.1016/j.dib.2019.104914 article EN cc-by-nc-nd Data in Brief 2019-11-30

In the State of Mato Grosso do Sul, Brazil, sand flies and cases visceral (VL) cutaneous (CL) leishmaniases have been reported in almost all municipalities. The aim this study was to analyze geographic distribution VL CL relation fly species found municipalities Sul.We analyzed from 2001 2018 using data Notifiable Diseases Information System (SINAN). Data collected since 2003 on presence vectors (proven or suspected) were provided by Health Secretariat.A total 3566 3030 CL, respectively,...

10.1186/s13071-022-05353-0 article EN cc-by Parasites & Vectors 2022-06-24

As Plantas Alimentícias Não Convencionais (PANC) são vegetais com potencial alimentício de crescimento espontâneo, que demandam aprofundamento quanto à propriedade nutricional, para valorização consumo e produção em escala industrial. A inserção PANC na formulação produtos alimentícios como barra cereais, pode fomentar o interesse um público ávido por novos funcionais. O objetivo foi desenvolver avaliar a estabilidade cereais formulada submetida irradiação Cobalto-60. barras foram submetidas...

10.55905/oelv22n4-136 article PT cc-by-nc OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA 2024-04-18
Coming Soon ...