A5040468977- Oral microbiology and periodontitis research
- Dental materials and restorations
- Oral Health Pathology and Treatment
- Endodontics and Root Canal Treatments
- Immune Response and Inflammation
- Dental Erosion and Treatment
- Streptococcal Infections and Treatments
- Antimicrobial Peptides and Activities
- NF-κB Signaling Pathways
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- HIV/AIDS oral health manifestations
- Dental Trauma and Treatments
- Oral and Maxillofacial Pathology
- Salivary Gland Disorders and Functions
- Bone and Dental Protein Studies
- Orthodontics and Dentofacial Orthopedics
- Bone Metabolism and Diseases
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Laser Applications in Dentistry and Medicine
- Surgical Sutures and Adhesives
- 3D Printing in Biomedical Research
- T-cell and Retrovirus Studies
- Microbial infections and disease research
University of Michigan
2024
Universidade Estadual Paulista (Unesp)
2011-2022
Centro Universitário de Araraquara
2010-2021
Morpho (United States)
2010
Background: Some haplotypes in the interleukin‐4 ( IL4 ) gene were reported to influence IL‐4 cytokine production and associated with inflammatory diseases. Association studies focusing on polymorphisms periodontal disease provided conflicting results. The aim of this study is investigate whether related chronic periodontitis a Brazilian population. Methods: –590(C/T) +33(C/T) identified by polymerase chain reaction (PCR) restriction fragment‐length polymorphism methods; variable number...
Abstract Fibronectin (FN)‐loaded nanofiber scaffolds were developed and assessed concerning their bioactive potential on human apical papilla cells (hAPCs). First, random (NR) aligned (NA) of polycaprolactone (PCL) obtained by electrospinning technique biological properties evaluated. The best formulations NR NA loaded with 0, 5, or 10 μg/ml FN bioactivity was assessed. Finally, FN‐loaded tubular prepared chemotactic analyzed using an in vitro model to mimic the pulp regeneration teeth...
Cytokines expression can be influenced by polymorphisms in their respective coding genes. We associated the CTI/TTD haplotype (Hap-1) and TCI/CCI (Hap-2) IL4 gene formed −590, +33 variable number of tandem repeat with severity chronic periodontitis humans. The functionality these haplotypes response immune cells to phorbol 12-myristate 13-acetate (PMA) Ionomycin IL-1β (as inflammatory stimuli) was evaluated. Gene (quantitative real-time PCR), profile secreted cytokines (multiplex) phenotypic...
SUMMARY Objectives To evaluate the influence of heat application on degree conversion (DC) 3M Single Bond Universal Adhesive System, as well its transdentinal cytotoxicity and microtensile bond strength to dentin. Methods Experimental groups were established according time temperature air jet: G1: 5 seconds–25°C; G2: 10 G3: 20 G4: seconds–50°C; G5: G6: seconds–50°C. In control group (G7), no treatment was performed. The DC assessed using Fourier transform infrared spectroscopy–attenuated...
Resin-modified glass ionomer cement (RMGIC) has important properties. However, like other restorative materials, it limitations such as decreased biocompatibility. The incorporation of nanoparticles (NP) in the RMGIC resulted improvements some its aim this study was to evaluate physical-biological properties with addition nanohydroxyapatite (HANP). Material and Methods: Vitremer used, incorporating HANP by amalgamator, vortex manual techniques, totaling ten experimental groups. distribution...
The chemokine receptor 1 CXCR-1 (or IL8R-alpha) is a specific for the interleukin 8 (IL-8), which chemoattractant neutrophils and has an important role in inflammatory response. polymorphism rs2234671 at position Ex2+860G > C of CXCR1 gene causes conservative amino acid substitution (S276T). This single nucleotide (SNP) seemed to be functional as it was associated with decreased lung cancer risk. Previous studies our group found association haplotypes IL8 CXCR2 genes multifactorial disease...
Chronic periodontitis (CP) is an infectious inflammatory disease that affects tooth-supporting structures and in which dental plaque bacteria, immune mechanisms genetic predisposition play important roles. Interleukin 4 (IL-4) a key anti-inflammatory cytokine with relevant action imbalances inflamed periodontal tissue. Individuals carrying the TCI/CCI genotype (S-haplotype) of IL-4 gene are 5 times more susceptible to CP, whereas CTI/TTD (P-haplotype) confers protection against CP. Compared...
The aim of this study was to investigate the effect non-surgical treatment periodontitis on levels periodontopathogens and clinical parameters in patients with different genetic backgrounds produced by polymorphisms Interleukin ( IL8 ) gene. Thirty grouped according ATC/TTC or AGT/TTC haplotypes were submitted periodontal treatment. Levels Porphyromonas gingivalis, Tannerella forsythia , Treponema denticola determined 240 subgingival plaque samples qPCR. association between investigated...
The present study evaluated the odontogenic potential of human dental pulp cells (HDPCs) exposed to chitosan scaffolds containing calcium aluminate (CHAlCa) associated or not with low doses simvastatin (SV). Chitosan received a suspension (AlCa) and were then immersed into solutions SV. following groups established: chitosan-calcium-aluminate (CHAlCa - Control), calcium-aluminate 0.5 µM SV (CHAlCa-SV0.5), 1.0 (CHAlCa-SV1.0). morphology composition by SEM EDS, respectively. After 14 days...
Background and Objective Although chronic periodontitis ( CP ) is a multifactorial condition, few studies have investigated the potential association of gene variants with outcome periodontal therapy. In previous study, we reported that in interleukin ‐ 8 IL were associated Brazilian population. The aim this nonrandomized study was to investigate whether genetic susceptibility , conferred by presence ATC / TTC haplotype, influences clinical outcomes nonsurgical therapy ‐8 protein levels...
The immune system detects shifts from homeostasis and eliminates altered cells. However, neoplastic cells can modulate the host response to escape immunosurveillance thereby allowing tumor progression. Head neck squamous cell carcinoma (HNSCC) is one of most immunosuppressive cancers but its role in co-opting actively promote growth has not been investigated. In this study, we investigated influence soluble factors secreted by HNSCC non-neoplastic epithelial on proliferation, apoptosis,...
This study aimed to evaluate the association between haplotypes in interleukin 8 (IL8) and IL4 genes previously associated chronic periodontitis (CP) levels of Aggregatibacter actinomycetemcomitans (A.a.) subgingival sites patients with without CP. Moreover, multifaceted evaluations were made search associations among patients' genetic background A.a. previous clinical/immunological/microbiological findings. Subgingival (n = 596) 104 divided into susceptible CP by IL8 haplotype ATC/TTC...
Non-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting outcomes. To compare proteolytic activity degradation rate of bovine dentin matrices.Dentin beam specimens were obtained from molars (n=30) incisors (n=30). The beams weighed hydrated after complete dehydration to obtain mineralized wet dry masses. Then, demineralized 10 wt% phosphoric acid. Next, 15 each...
The current study evaluated the effects of taxifolin treatments on viability osteoblast-like cells, and expression early mineralization markers, as part ongoing search for new endodontic materials able to induce periapical healing without causing cytotoxicity. Saos-2 cells were exposed different concentrations (5 10 µM), applied pretreatments either 24h 72h, or continuously throughout experimental protocol. Cell using methylthiazole tetrazolium (MTT) assay, alkaline phosphatase activity...