A5084579289- Advanced Radiotherapy Techniques
- Cancer Cells and Metastasis
- Radiation Therapy and Dosimetry
- Prostate Cancer Diagnosis and Treatment
- Medical Imaging Techniques and Applications
- Bladder and Urothelial Cancer Treatments
- Head and Neck Cancer Studies
- Prostate Cancer Treatment and Research
- Advanced X-ray and CT Imaging
- Protease and Inhibitor Mechanisms
- Cancer Genomics and Diagnostics
- Radiation Dose and Imaging
- Radiomics and Machine Learning in Medical Imaging
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Genomics and Phylogenetic Studies
- Advanced MRI Techniques and Applications
- Lung Cancer Diagnosis and Treatment
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Medical Imaging and Analysis
- Organometallic Complex Synthesis and Catalysis
- Colorectal Cancer Surgical Treatments
- RNA and protein synthesis mechanisms
- Cancer Diagnosis and Treatment
Royal Marsden NHS Foundation Trust
2020-2025
Sutton Hospital
2021-2024
Royal Marsden Hospital
2020-2022
Teledyne FLIR (United States)
2014-2016
San Diego State University
2009
University of California, San Diego
2001-2006
National Institutes of Health
1999
IntroductionMR-guided adapted radiotherapy (MRgART) using a high field MR-linac has recently become available. We report the estimated delivered fractional dose of first five prostate cancer patients treated at our centre MRgART and compare this to C-Arm linac daily Image Guided Radiotherapy (IGRT).MethodsPatients were treatment plans shaped their anatomy. The treatments recalculated on an MR image acquired immediately prior delivery in order estimate dose. C-arm non-adapted VMAT same images...
v-Src transforms fibroblasts in vitroand causes tumor formation the animal by tyrosine phosphorylation of critical cellular substrates. Exactly how interacts with these substrates remains unknown. One its substrates, adaptor protein Shc, is thought to play a crucial role during transformation linking Ras. We used Shc proteins mutations either phosphotyrosine binding (PTB) or Src homology 2 domain determine that v-Src-expressing cells depends on presence functional PTB domain. purified...
To measure the gradient system transfer function (GSTF) of an MR-Linac (Elekta Unity, Stockholm, Sweden) using accessible phantom-based method and to apply trajectory corrections for UTE image reconstruction in context MR-guided radiotherapy lung cancer. The first-order GSTF a 1.5 T, split Elekta Unity was measured thin-slice technique characterize imperfections each physical axis (X, Y, Z). Repeatability measurements were performed 48 h apart. applied correction multi-echo (TEs = 0.176,...
The low density lipoprotein receptor-related protein 1 (LRP1) mediates internalization of a large number proteins and protein-lipid complexes is widely implicated in Alzheimer's disease. cytoplasmic domain LRP1 (LRP1-CT) can be phosphorylated by activated protein-tyrosine kinases at two NPXY motifs LRP1-CT; Tyr 4507 readily must before phosphorylation 4473 occurs. Pull-down experiments from brain lysate revealed numerous binding to LRP1-CT, but the results were highly variable. To separate...
Whole bladder magnetic resonance image-guided radiotherapy using the 1.5 Telsa MR-linac is feasible. Full online adaptive planning workflow based on anatomy seen at each fraction was performed. This delivered within 45 min. Intra-fraction filling did not compromise target coverage. Patients reported acceptable tolerance of treatment.
The abundance of circulating tumor cells (CTC) indicates patient prognosis. Molecular characterization CTCs may add additional information about a patient's disease. However, currently available methods are limited by contamination with blood cells. We describe study using modified CTC-chip to capture from an orthotopic xenograft model. Using laser microscopy collect the chip, we compared transcripts purified those primary and metastatic tissue. Transcriptional profiles showed strong...
The Elekta Unity MR-Linac (MRL) has enabled adaptive radiotherapy (ART) for patients with head and neck cancers (HNC). Adapt-To-Shape-Lite (ATS-Lite) is a novel Adapt-to-Shape strategy that provides ART without requiring daily clinician presence to perform online target organ at risk (OAR) delineation. In this study we compared the performance of our clinically-delivered ATS-Lite against three Adapt-To-Position (ATP) variants: Adapt Segments (ATP-AS), Optimise Weights (ATP-OW), Shapes (ATP-OS).
IntroductionWith the implementation of new radiotherapy technology, it is imperative that patient experience investigated alongside efficacy and outcomes. This paper presents development a specifically designed validated questionnaire first report international multi-institutional preliminary MRI-guided adaptive (MRgART) on 1.5 T MR-Linac (MRL).MethodsA was developed before being distributed to Elekta MRL Consortium, gather patient-reported from participating centres worldwide. The final...
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, use DpnI immobilized on magnetic beads. The ten minute extraction technique allows genomes containing Gm6ATC motif common in genomic many bacteria including γ-proteobacteria. Using synthetic genome mixtures, demonstrate 80% recovery Escherichia coli even when only femtogram quantities are spiked into 10 µg human background. Binding...
We describe continuing work to develop restriction endonucleases as tools enrich targeted genomes of interest from diverse populations. Two approaches were developed in parallel segregate genomic DNA based on cytosine methylation. First, the methyl-sensitive endonuclease HpaII was used bind non-CG methylated DNA. Second, a truncated fragment McrB CpG Enrichment levels microbial can exceed 100-fold with allowing improved detection and coverage otherwise trace sputum. Additionally, we observe...
The MR-Linac (MRL) provides a novel treatment modality that enables online adaptive treatments, but also creates new challenges for patient positioning in laser-free environment. accuracy and duration of prostate set-up on the MRL using two different methods alignment was determined to establish standard practice MRL. Differences were significant longitudinal direction are accounted plan adaption. Both recorded similar times. vendor recommended method involves less manipulation will be...
The rotational structure in the C-type bands of high-resolution (0.002 cm-1), gas-phase infrared spectra four isotopomers trans-1,2-dichloroethylene has been analyzed. band, which is due to CH or CD out-of-plane flapping, at 897.949 82 (3) cm-1 for normal species with 35Cl2. For 35Cl2 variants, ground-state constants are A = 1.747 268 (3), B 0.051 570 5 and C 0.050 080 7 species; 1.677 033 (2), 501 0 0.049 954 (2) 13C2 1.181 144 (9), 540 371 9 d2 species. d2-35Cl37Cl isotopomer, 1.180 765...
Neoadjuvant chemoradiotherapy followed by surgery is the mainstay of treatment for patients with rectal cancer. Standard clinical target volume (CTV) to planning (PTV) margins 10 mm are used accommodate inter- and intrafraction motion target. Treating on magnetic resonance-integrated linear accelerators (MR-linacs) allows online manual recontouring adaptation (MRgART) enabling reduction PTV margins. The aim this study was investigate primary CTV (CTVA; gross tumour macroscopic nodes...
The reaction of [(PPh3)2Pt(η3-CH2CCPh)]X (X = CF3SO3-, BF4-, PF6-) with CNR (R C(CH3)3, CH2Ph) yields a mixture the η1-propargyl and -allenyl complexes trans-[(PPh3)2Pt(η1-CH2C⋮CPh)(CNR)]+ trans-[(PPh3)2Pt(η1-CPhCCH2)(CNR)]+, respectively, in about 6/1 proportion. Isolation pure propargyl complex was possible several cases X CF3SO3-; R CH2Ph, PF6-). In solution cationic trans-[(PPh3)2Pt(η1-CH2C⋮CPh)(CNC(CH3)3)]+ rearranges to an indenyl which isolated as triflate salt...
IntroductionSwallow-related motion of the larynx is most significant in cranio-caudal directions and short duration. Conventional target definition for radical radiotherapy includes coverage whole larynx. This study longitudinally examined respiration- swallow-related laryngeal motions using cine-MRI. We further analysed dosimetry to organs at risk by comparing 3D-conformal (3D-CRT), Volumetric-Modulated Arc Therapy (VMAT) Intensity-Modulated Radiotherapy (IMRT) techniques.MethodsFifteen...