A5042900997- Adipose Tissue and Metabolism
- Nuclear Structure and Function
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Bone Metabolism and Diseases
- Diet and metabolism studies
- Bone health and osteoporosis research
- Blood properties and coagulation
- Bone and Joint Diseases
- Skin and Cellular Biology Research
- Genomics and Chromatin Dynamics
- Platelet Disorders and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Estrogen and related hormone effects
- Glycosylation and Glycoproteins Research
- Dietary Effects on Health
- Exercise and Physiological Responses
- Molecular Biology Techniques and Applications
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Nutrition and Health in Aging
- Cell Adhesion Molecules Research
- Hip disorders and treatments
- Lipid metabolism and disorders
- Muscle metabolism and nutrition
- Diabetes and associated disorders
University of North Carolina at Chapel Hill
2016-2024
Centers for Disease Control and Prevention
1981
Abstract Polymerized β-actin may provide a structural basis for chromatin accessibility and actin transport into the nucleus can guide mesenchymal stem cell (MSC) differentiation. Using MSC, we show that using CK666 to inhibit Arp2/3 directed secondary branching results in decreased nuclear structure, significantly alters access measured with ATACseq at 24 h. The ATAC-seq due are distinct from those caused by cytochalasin D (CytoD), which enhances structure. In addition, visualization shows...
The relationship between marrow adipose tissue (MAT) and bone health is poorly understood. We used running exercise to ask whether obesity-associated MAT can be attenuated via this correlates with gains in quantity quality. C57BL/6 mice were divided into diet-induced obesity (DIO, n = 14) versus low-fat diet (LFD, 14). After 3 months, 16-week-old allocated an intervention (LFD-E, DIO-E) or a control group DIO) for 6 weeks (4 groups, 7/group). Marrow adipocyte area was 44% higher (p < 0.0001)...
Actin structure contributes to physiologic events within the nucleus control mesenchymal stromal cell (MSC) differentiation. Continuous cytochalasin D (Cyto D) disruption of MSC actin cytoskeleton leads osteogenic or adipogenic differentiation, both requiring mass transfer into nucleus. Cyto remains extranuclear, thus intranuclear polymerization is potentiated by transfer: we asked whether affects We show that secondary filament branching via Arp2/3 complex required for osteogenesis and...
ABSTRACT Marrow adipose tissue (MAT) and its relevance to skeletal health during caloric restriction (CR) is unknown: It remains unclear whether exercise, which anabolic bone in a calorie-replete state, alters or MAT CR. We hypothesized that response of exercise CR differs from the state. Ten-week-old female B6 mice fed regular diet (RD) 30% were allocated sedentary (RD, CR, n = 10/group) running (RD-E, CR-E, 7/group). After 6 weeks, weighed 20% less than RD, p &lt; 0.001; did not affect...
<b><i>Introduction:</i></b> Longitudinal effect of diet-induced obesity on bone is uncertain. Prior work showed both no and a decrement in density or quality when begins prior to skeletal maturity. We aimed quantify long-term effects marrow adipose tissue (BMAT) adulthood. <b><i>Methods:</i></b> Skeletally mature, female C57BL/6 mice (<i>n</i> = 70) aged 12 weeks were randomly allocated low-fat diet (LFD; 10% kcal fat;...
Muscle Lipid increases with high-fat feeding and diabetes. In trained athletes, increased muscle lipid is not associated insulin resistance, a phenomenon known as the athlete's paradox. To understand if exercise altered phenotype of lipid, female C57BL/6 mice fed CTL or high fat diet (HFD for 6 18 wk) were further divided into sedentary exercising groups (CTL-E HFD-E) voluntary access to running wheels last wk experiments, h/night. Diet did affect time distance. HFD weighed more than after...
Nuclear actin plays a critical role in mediating mesenchymal stem cell (MSC) fate commitment. In marrow-derived MSCs, the principal diaphanous-related formin Diaph3 (mDia2) is present nucleus and regulates intranuclear polymerization, whereas Diaph1 (mDia1) localized to cytoplasm controls cytoplasmic polymerization. We here show that mDia2 can be used as tool query actin-lamin nucleoskeletal structure. Silencing affected lamin scaffold, altering nuclear morphology without affecting...
ABSTRACT Use of the selective estrogen receptor modulator Tamoxifen (TAM) is a mainstay to induce conditional expression Cre recombinase in transgenic laboratory mice. To excise β‐catenin fl/fl 28‐day‐old male and female Prrx1‐CreER/β‐catenin mice (C57BL/6), we utilized TAM at 150 mg/kg; despite knockout MSC, found significant increase trabecular cortical bone volume all genders. Because was similarly anabolic KO control mice, investigated dose effect on formation by treating wild‐type (WT...
Exercise, typically beneficial for skeletal health, has not yet been studied in lipodystrophy, a condition characterized by paucity of white adipose tissue, with eventual diabetes, and steatosis. We applied mouse model global deficiency Bscl2 (SEIPIN), required lipid droplet formation. Male twelve-week-old B6 knockouts (KO) wild type (WT) littermates were assigned six-weeks voluntary, running exercise (E) versus non-exercise (N=5-8). KO weighed 14% less than WT (p=0.01) exhibited an absence...
Studies in our laboratories have been concerned with the responses of megakaryocytes to thrombopoietin vitro. We shown that preparations stimulate DNA synthesis guinea pig megakaryocytes. The increase 3H-thymidihe incorporation correlates an labeling index After 2 and 3 days incubation ploidy levels has observed thrombo- poietin-supplemented cultures compared controls. Recent studies examined 3H-leucine megakaryocyte cultures. Megakaryocytes were prepared on BSA or Percoll gradients purities...
Liquid cultures of bone marrow cells enriched for megakaryocytes were assayed incorporation 3H-thymidine (3H-TdR) into acid-precipitable cell digests to determine the effect thrombopoietin on DNA synthesis. As previously described, was prepared by ammonium sulfate fractionation pooled plasma obtained from thrombocytopenic rabbits. A control fraction normal rabbit plasma. The thrombopoietic activity these fractions determined in vivo with rabbits as assay animals and rate...