A5101762423- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Medicinal Plant Pharmacodynamics Research
- Bioenergy crop production and management
- Seed Germination and Physiology
- Bacterial biofilms and quorum sensing
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Genomics and Chromatin Dynamics
- Telomeres, Telomerase, and Senescence
- Fungal and yeast genetics research
- Retinal Diseases and Treatments
- Plant-Microbe Interactions and Immunity
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
Northeast Forestry University
2012-2024
BaiCheng Normal University
2024
Ministry of Agriculture and Rural Affairs
2024
Jimei University
2024
People’s Hospital of Rizhao
2024
Binzhou University
2014-2023
Binzhou Medical University
2014-2023
Nanjing University of Chinese Medicine
2023
Jiangsu Province Hospital
2023
Beijing Municipal Ecological and Environmental Monitoring Center
2023
Long noncoding RNAs (lncRNAs) play important roles in the development of vascular diseases.However, effect lncRNA NORAD on atherosclerosis remains unknown.This study aimed to investigate endothelial cell injury and atherosclerosis.Ox-LDL-treated human umbilical vein cells (HUVECs) high-fat-diet (HFD)-fed ApoE -/-mice were used as vitro vivo models.Results showed that NORAD-knockdown induced cycle arrest G0/G1 phase, aggravated ox-LDL-induced viability reduction, apoptosis, senescence along...
Heart failure (HF) is a deadly disease that difficult to accurately diagnose. Circular RNAs (circRNAs) are novel class of noncoding might play important roles in many cardiovascular diseases. However, their role HF remains unclear. CircRNA microarrays were performed on plasma samples obtained from three patients with and healthy controls. The profiling results validated by quantitative reverse transcription polymerase chain reaction. diagnostic value circRNAs for was evaluated receiver...
Micro-Tom is the smallest known variety of tomatoes.An orthogonal experimental design L 16 (4 5 ) was used to optimize Agrobacterium-mediated transformation cotyledon explants Lycopersicon esculentum cv.Micro-Tom.Four parameters were investigated determine their effect on frequency: concentration bacterial suspension, time dip in co-cultivation time, and carbenicillin.We also examined these contamination rate, necrosis mortality, cutsurface browning undamaged explant rate.Both carbenicillin...
Earlier work showed that apoptosis of alveolar epithelial cells (AECs) in response to endogenous or xenobiotic factors is regulated by autocrine generation angiotensin (ANG) II and its counterregulatory peptide ANG1-7. Mutations surfactant protein C (SP-C) induce endoplasmic reticulum (ER) stress AECs cause lung fibrosis. This study tested the hypothesis ER stress-induced might also be ANGII/ANG1-7 system AECs. was induced A549 primary cultures human with proteasome inhibitor MG132 SP-C...
Real-time Quantitative PCR (RT-qPCR) has become an effective method for accurate analysis of gene expression in several biological systems as well under different experimental conditions. Although with high sensitivity, specificity and broad dynamic range, this requires suitable reference genes transcript normalization order to guarantee reproducible meaningful results. In the present study, we evaluated five traditional housekeeping novel Hyperaccumulating ecotype Sedum alfredii, a known...
The TIFY family is a plant-specific gene and plays an important role in plant growth development. But few reports have been reported on the phylogenetic analysis expression profiling of genes birch (Betula platyphylla).In this study, we characterized identified 12 using phylogeny chromosome mapping birch. members were divided into JAZ, ZML, PPD subfamilies. Phylogenetic revealed that clustered six evolutionary branches. distribution showed unevenly distributed 5 chromosomes. Some derived...
Eukaryotic gene expression is often tightly regulated by interactions between transcription factors (TFs) and their DNA cis targets. Yeast one-hybrid (Y1H) one of the most extensively used methods to discover these interactions. We developed a high-throughput meiosis-directed yeast system using Magic Markers synthetic genetic array analysis. The has factor–DNA interaction discovery rate twice as high conventional diploid-mating approach processing time nearly one-tenth haploid-transformation...
Pantoea agglomerans YS19, an endophytic diazotrophic bacterium isolated from rice, is characterized by the formation of multicellular aggregate structure called symplasmata, which not only bestow strong stress‐resistance bacterium, but also contribute to specific adaptation in endophyte–host association. Acyl‐homoserine lactones (AHLs), as important signal molecule quorum sensing (QS) system gram‐negative bacteria, were demonstrated regulate motility, cell‐aggregation, and other bacterial...
Background Dendrocalamus latiflorus Munro distributes widely in subtropical areas and plays vital roles as valuable natural resources. The transcriptome sequencing for D. has been performed numerous genes especially those predicted to be unique were revealed. qRT-PCR become a feasible approach uncover gene expression profiling, the accuracy reliability of results obtained depends upon proper selection stable reference accurate normalization. Therefore, set suitable internal controls should...
Pantoea agglomerans YS19 is an endophytic diazotrophic bacterium isolated from rice. As well as having the ability to form a biofilm, do most bacteria, it characterized by formation of unique multicellular aggregate structure called symplasmata. Indole traditionally known metabolite amino acid tryptophan, which, however, has recently been shown participate in various regulations bacterial physiological processes, including stress resistance, quorum sensing and biofilm formation. Here, indole...
Recently, long noncoding RNAs (lncRNAs) have been key regulators for both mRNAs and proteins in nucleated cells. However, the expression profiles of lncRNAs non-nucleated cells such as platelets are currently unclear. In this study, we determined human platelets. We found that 6109 were expressed Interestingly, 338 differentially hyperreactive hyporeactive Bioinformatics' analysis revealed these aberrantly might be related to platelet activity other functions. To provide a proof concept,...