A5011018685- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Congenital heart defects research
- Gut microbiota and health
- Developmental Biology and Gene Regulation
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- Retinal Development and Disorders
- Cardiomyopathy and Myosin Studies
- Kruppel-like factors research
- Microbial Inactivation Methods
- Genetics and Neurodevelopmental Disorders
- Microtubule and mitosis dynamics
- Vibrio bacteria research studies
- Axon Guidance and Neuronal Signaling
- Genetic diversity and population structure
- Evolution and Genetic Dynamics
- Genetic Syndromes and Imprinting
- Neuroendocrine regulation and behavior
- Diet and metabolism studies
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Fibroblast Growth Factor Research
- Autism Spectrum Disorder Research
- Echinoderm biology and ecology
University of Guelph
2014-2024
Vanderbilt University
2005-2011
Vanderbilt University Medical Center
1997-2011
University of Utah
2001-2005
Addenbrooke's Hospital
1997
Johns Hopkins University
1997
Sequenom (United States)
1996
Journal Article Analysis of the genomic sequence for autosomal dominant polycystic kidney disease (PKD1) gene predicts presence a leucine-rich repeat: The AMERICAN PKD1 Consortium (APKD1 Consortium) Get access Timothy C. Burn, Burn Search other works by this author on: Oxford Academic PubMed Google Scholar D. Connors, Connors William R. Dackowski, Dackowski Linda Petry, Petry Terence J. van Raay, Raay John M. Millholland, Millholland Marc Venet, Venet Glenn Miller, Miller Ramond Hakim, Hakim...
The primary structure of polycystin predicts a large integral membrane protein with multiple cell recognition motifs, but its function remains unknown. Insight into polycystin’s normal and role in the development autosomal dominant polycystic kidney disease (PKD1) requires assembly an extensive collection molecular reagents to examine expression create model systems for functional studies. Development these crucial has been complicated due presence transcriptionally active homologous loci....
The Wnt signaling pathway is critical in normal development, and mutation of specific components frequently observed carcinomas diverse origins. However, the potential involvement this lung tumorigenesis has not been established. In study, analysis multiple mRNAs non-small cell cancer (NSCLC) lines primary tumors revealed markedly decreased Wnt-7a expression compared with short-term bronchial epithelial uninvolved tissue. transfection NSCLC reversed cellular transformation,...
In Drosophila, naked cuticle is an inducible antagonist of the Wnt-beta-catenin pathway, likely acting at level Dishevelled (Dsh/Dvl), essential component this pathway. The mechanism by which and its two vertebrate orthologs, Naked1 (NKD1) Naked2 (NKD2), inhibit Dvl function unknown. NKD2 myristoylated, a co-translational modification that leads to plasma membrane localization. contrast, myristoylation-deficient G2A cytoplasmic. Herein we show ability Nkd2/NKD2 antagonize activity during...
Cyto-nuclear shuttling of β-catenin is at the epicenter canonical Wnt pathway and mutations in genes that result excessive nuclear accumulation are driving force behind initiation many cancers. Recently, Naked Cuticle homolog 1 (Nkd1) has been identified as a Wnt-induced intracellular negative regulator signaling. The current model suggests Nkd1 acts between Disheveled (Dvl) β-catenin. Here, we employ zebrafish embryo to characterize cellular biochemical role vivo. We demonstrate binds...
Wnt signaling is involved in many aspects of development and the homeostasis stem cells. Its importance underscored by fact that misregulation has been implicated numerous diseases, especially colorectal cancer. However, how regulates itself not well understood. There are several negative feedback regulators, which active antagonists signaling, but one regulator, Nkd1, reduced activity compared to other antagonists, yet still a regulator. Here we describe our efforts understand role Nkd1...
The zebrafish has become increasingly popular for microbiological research. It been used as an infection model a variety of pathogens, and is also emerging tool studying interactions between host its resident microbial communities. mouse microbiota transplanted into the gut, but to our knowledge, there no attempt introduce bacterial community derived from human gut. We explored two methods colonizing developing gut 5-day-old germ-free larvae with defined anaerobic single fecal sample. Both...
Misregulation of Wnt signaling is at the root many diseases, most notably colorectal cancer, and although we understand activation pathway, have a very poor understanding circumstances under which turns itself off. There are numerous negative feedback regulators signaling, but two stand out as constitutive obligate Wnt-induced regulators: Axin2 Nkd1. Whereas behaves similarly to Axin in destruction complex, Nkd1 more enigmatic. Here use zebrafish blastula cells that responsive demonstrate...
Wnt signaling is a crucial developmental pathway involved in early development as well stem-cell maintenance adults and its misregulation leads to numerous diseases. Thus, understanding the regulation of this becomes vitally important. Axin2 Nkd1 are widely utilized negative feedback regulators where functions destabilize cytoplasmic β-catenin, inhibit nuclear localization β-catenin. Here, we set out further understand how regulate by creating
The hemicyanine hybrid containing the 7-(diethylamino)coumarin (ACou) donor attached to cationic indolenium (Ind) acceptor through a vinyl linkage (ACou-Ind) represents classic ratiometric fluorescent probe for detecting nucleophilic analytes, such as cyanide and reactive sulfur species (RSS), addition reactions that disrupt dye conjugation turn off red internal charge transfer (ICT) fluorescence on blue coumarin emission. chemosensing mechanism RSS detection by ACou-Ind suggested in...
Abstract Background Small molecule metabolites produced by the microbiome are known to be neuroactive and capable of directly impacting brain central nervous system, yet there is little data on contribution these earliest stages neural development gene expression. Here, we explore impact deriving zebrafish embryos in absence microbes early as well investigate whether any potential changes can rescued with treatment derived from gut microbiota. Results Overall, did not observe gross...
A pyrimidine-rich element (PyRE), present in the 21st intron of PKD1 gene, posed a significant obstacle determining primary structure gene. Only cycle sequencing nested, single-stranded phage templates CT-rich strand enabled complete and accurate sequence data. Similar attempts on GA-rich were unsuccessful. The resulting showed 3 kb to contain 2.5 PyRE, whose sense-strand is 97% C + T. PyRE does not appear be polymorphic based RFLP analysis DNA from 6 unrelated individuals digested with 9...
The construction of far-red fluorescent molecular rotors (FMRs) is an imperative task for developing nucleic acid stains that have superior compatibility with cellular systems and complex matrices. A typical strategy relies on the methine extension asymmetric cyanines, which unfortunately fails to produce sensitive rotor character. To break free from this paradigm, we synthesized hemicyanines using a dimethylamino thieno[3,2-
Settlement is a rapid process in many marine invertebrate species, transitioning planktonic larva into benthic juvenile. In indirectly developing sea urchins, this ecological transition correlates with morphological, developmental and physiological (metamorphosis) during which apoptosis essential for the resorption remodelling of larval juvenile structures. While settlement initiated by environmental cues (i.e. habitat-specific or substrate cues), metamorphosis regulated endocrine signals,...