A5100739096- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Gene expression and cancer classification
- Glycosylation and Glycoproteins Research
- Genomic variations and chromosomal abnormalities
- Photosynthetic Processes and Mechanisms
- Cancer-related gene regulation
- Plant Virus Research Studies
- RNA Research and Splicing
- Extracellular vesicles in disease
- Plant tissue culture and regeneration
- Metabolism and Genetic Disorders
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Cancer Immunotherapy and Biomarkers
- Plant Molecular Biology Research
- Virus-based gene therapy research
- Prenatal Screening and Diagnostics
- Research in Cotton Cultivation
Xidian University
2022-2025
Wuhan University
2022-2023
Henan Institute of Science and Technology
2020-2023
Soochow University
2011-2023
Liaocheng University
2022-2023
Tobacco Research Institute
2022
Chinese Academy of Agricultural Sciences
2022
State Key Laboratory of Hybrid Rice
2022
Hubei Provincial Women and Children's Hospital
2009-2018
Kingmed Diagnostics
2018
Although the tremendous progress has been made for mRNA delivery based on classical cationic carriers, excess charge density of lipids was necessary to compress through electrostatic interaction, and with it comes inevitably adverse events including highly inflammatory cytotoxic effects. How develop disruptive technologies overcome nature remains a major challenge safe efficient delivery. Here, we prepared noncationic thiourea nanoparticles (NC-TNP) by strong hydrogen bonds interaction...
The history of the Newborn Screening Program in Mainland China begins 1981, when a pilot plan was developed that demonstrated feasibility its implementation. It has so far focused on detection congenital hypothyroidism (CH) and phenylketonuria (PKU) to prevent or reduce mental physical developmental retardation children. Throughout this period, total 35,795,550 dried blood samples (DBS) newborns (NB) have been analyzed for PKU, 35,715,988 CH. During 3,082 cases with PKU diagnosed, resulting...
Here, in an effort towards facile and fast screening/diagnosis of novel coronavirus disease 2019 (COVID-19), we combined the unprecedently sensitive graphene field-effect transistor (Gr-FET) with highly selective antibody-antigen interaction to develop a immunosensor. The Gr-FET immunosensors can rapidly identify (about 2 mins) accurately capture COVID-19 spike protein S1 (which contains receptor binding domain, RBD) at limit detection down 0.2 pM, real-time label-free manner. Further...
Diosgenin saponins isolated from Dioscorea species such as D. zingiberensis exhibit a broad spectrum of pharmacological activities. Diosgenin, the aglycone diosgenin saponins, is an important starting material for production steroidal drugs. However, how plants produce and origin evolution saponin biosynthetic pathway remain mystery. Here we report high-quality, 629-Mb genome anchored on 10 chromosomes with 30 322 protein-coding genes. We reveal that synthesized in leaves ('source'), then...
Grain size is one of the most important factors affecting rice grain quality and yield, attracts great attention from molecular biologists breeders. In this study, we engineered a CRISPR/Cas9 system targeting miR396 recognition site GS2 gene, which encodes growth-regulating factor 4 (OsGRF4) regulates multiple agronomic traits including size, quality, nitrogen use efficiency, abiotic stress response, seed shattering. contrast to previous genome editing efforts in indel mutations were chosen...
Development of anticancer treatments based on microRNA (miRNA/miR) such as miR-34a replacement therapy is limited to the use synthetic RNAs with artificial modifications. Herein, we present a new approach high-yield and large-scale biosynthesis, in <i>Escherichia coli</i> using transfer RNA (tRNA) scaffold, chimeric agent, which may act prodrug for therapy. The recombinant tRNA fusion pre–miR-34a (tRNA/mir-34a) was quickly purified high degree homogeneity (>98%) anion-exchange fast...
Abstract Whitefly-transmitted begomoviruses are economically important plant pathogens that cause severe problems in many crop plants, such as tomato, papaya, cotton, and tobacco. Tomato yellow leaf curl virus (TYLCV) is a typical monopartite begomovirus has been extensively studied, but methods can efficiently control still scarce. In this study, we combined artificial microRNA (amiRNA)-mediated silencing technology clay nanosheet-mediated delivery by spraying developed method for...
Effective repolarization of macrophages has emerged as a promising approach for anticancer therapy. However, there are very few studies on the effect reprogramming from M2 phenotype to M1 without reconversion while maintaining an activated phenotype. Moreover, these immunomodulatory methods have serious drawbacks due activation normal monocytic cells. Therefore, it remains challenge selectively reprogram tumor-associated (TAMs) systemic toxicities. Here, X-ray-guided and triggered remote...
Trehalose-6-phosphate phosphatase (TPP) is a key enzyme involved in trehalose synthesis higher plants. Previous studies have shown that TPP family genes increase yields without affecting plant growth under drought conditions, but their functions cotton not been reported. In this study, 17, 12, 26 and 24 were identified Gossypium arboreum, raimondii, barbadense hirsutum, respectively. The 79 divided into three subgroups by phylogenetic analysis. Virus-induced gene silencing (VIGS) of GhTPP22...
Efferocytosis of apoptotic tumor cells by tumor-associated macrophages mediated through the phosphatidylserine (PtdSer)/MER proto-oncogene tyrosine kinase (MerTK) axis can exacerbate immunosuppression, and conversely, prevention efferocytosis via blocking PtdSer-MerTK association using prevalent antibodies represents a promising strategy for reversing immunosuppression boosting antitumor immunity. However, it remains unclear whether antibody blockade induce durable achieve sustained growth...
Colorectal cancer (CRC) is one of the most common malignancies in world. Studies have demonstrated that single nucleotide polymorphisms (SNPs) microRNA genes (miRSNPs) are involved occurrence cancers. However, relationship between miRSNPs within terminal-loops precursors and development CRC still largely unknown. In this study, we found a miRSNP rs7911488 T>C terminal-loop pre-miR-1307 was significantly associated with CRC. The C allele more prevalent patients than healthy controls (P <...
Although biomacromolecules are promising cytosolic drugs which have attracted tremendous attention, the major obstacles were cellular membrane hindering entrance and endosome entrapment inducing biomacromolecule degradation. How to avoid those limitations realize directly delivery was still a challenge. Here, we prepared oligoarginine modified lipid assemble nanovesicle for delivery, including mRNA (mRNA) proteins could be delivered into cytoplasm of dendritic cells through...
In pyrosequencing chemistry, four cascade enzymatic reactions with the catalysis of polymerase, adenosine triphosphate (ATP) sulfurylase, luciferase, and apyrase are employed. The sensitivity mainly depends on concentration luciferase which catalyzes a photoemission reaction. However, side-reaction 5′ phosphosulfate (APS, an analogue ATP) resulted in unavoidable background signal; hence, cannot be much higher due to simultaneous increase signal when larger amount is used. this study, we...
To investigate the association of microRNA (miRNA) binding-site polymorphisms in drug transporter genes with efficacy 5-Fluorouracil (5-FU)/capecitabine-based chemotherapy colorectal cancer (CRC), 6 were determined 432 CRC patients by using DNA sequencing method. The impacts on miRNA-mediated regulation gene expression evaluated methods including quantitative real-time PCR, western blotting, and luciferase reporter assays. effects miRNA intracellular concentration cytotoxicity 5-FU cells...
Robertsonian translocations occur in approximately one every 1000 newborns. Although most translocation carriers are healthy and have a normal lifespan, they at increased risk of spontaneous abortions producing unbalanced gametes and, therefore offspring. Here we reported previously undescribed translocation.We identified three this family. Two were heterozygous 45,XX or XY,der(14;15)(q10;q10) their son was homozygous carrier 44,XY,der(14;15)(q10;q10), der(14;15)(q10;q10) karyotype....