A5006572085- Indoor and Outdoor Localization Technologies
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Advanced Control Systems Optimization
- Fault Detection and Control Systems
- Monoclonal and Polyclonal Antibodies Research
- Stability and Control of Uncertain Systems
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Blockchain Technology Applications and Security
- Medical Research and Treatments
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Animal Virus Infections Studies
- Traditional Chinese Medicine Studies
- Extracellular vesicles in disease
- Listeria monocytogenes in Food Safety
- SARS-CoV-2 and COVID-19 Research
- Renal Diseases and Glomerulopathies
- Cloud Data Security Solutions
Guangdong Provincial Center for Disease Control and Prevention
2008-2025
RELX Group (Netherlands)
2022-2025
The First Affiliated Hospital, Sun Yat-sen University
2022-2025
South China University of Technology
2025
Guangzhou Medical University
2011-2025
Guangdong Medical College
2001-2025
Guangzhou First People's Hospital
2025
Wuhan University of Science and Technology
2025
Hangzhou Seventh Peoples Hospital
2024
Zhejiang University
2011-2024
The genomic sequences of severe acute respiratory syndrome coronaviruses from human and palm civet the 2003/2004 outbreak in city Guangzhou, China, were nearly identical. Phylogenetic analysis suggested an independent viral invasion animal to this new episode. Combining all existing data but excluding singletons, we identified 202 single-nucleotide variations. Among them, 17 are polymorphic civets only. ratio nonsynonymous/synonymous nucleotide substitution collected 1 yr apart different...
Ultra-sensitive detection of cancer-related biomarkers in serum is great significance for early diagnosis, treatment, prognosis, and staging cancer. In this work, we proposed a surface-enhanced Raman scattering fluorescence (SERS/FL) dual-mode biosensor hepatocellular carcinoma (HCC)-related miRNA (miR-224) using the composition well-arranged Au nanoarrays (Au NAs) substrate coupled with target-catalyzed hairpin assembly (CHA) strategy. The hot spots densely uniformly distributed on array...
Purpose The successful and commercial use of self-driving/driverless/unmanned/automated car will make human life easier. paper aims to discuss this issue. Design/methodology/approach This reviews the key technology a self-driving car. In paper, four technologies in car, namely, navigation system, path planning, environment perception control, are addressed surveyed. main research institutions groups different countries summarized. Finally, debates discussed development trend is predicted....
Predicting the accurate traffic crowd flows is of practical importance for intelligent transportation systems (ITS). However, it challenging because are affected by multiple complex factors, such as spatial and temporal dependencies regions external factors. In this paper, we propose a deep hybrid spatio-temporal dynamic neural network, called DHSTNet, to predict both inflows outflows in every region city. More specifically, consists four main components, i.e., closeness influence taking...
In situ and real-time monitoring of responsive drug release is critical for the assessment pharmacodynamics in chemotherapy. this study, a novel pH-responsive nanosystem proposed chemo-phototherapy by surface-enhanced Raman spectroscopy (SERS). The Fe3O4@Au@Ag nanoparticles (NPs) deposited graphene oxide (GO) nanocomposites with high SERS activity stability are synthesized labeled reporter 4-mercaptophenylboronic acid (4-MPBA) to form probes (GO-Fe3O4@Au@Ag-MPBA). Furthermore, doxorubicin...
Green technological innovation is one of the endogenous drivers green economic growth, and digitalization can promote development in form industrial empowerment. The interactive relationship degree influence between digitalization, innovation, thus an urgent issue to be addressed. Based on panel data 30 Chinese provinces from 2011 2019, we measured for first time using entropy method included them same analytical framework by constructing a PVAR model empirically test their interrelationship...
Agricultural production is closely related to the development of country. With continuous science and technology, China's facility agriculture advancing rapidly. Facility can overcome impact natural environment improve efficiency. The only way for China realize agricultural modernization. Greenhouses in have been widely promoted, but degree automation not high. intelligent algorithms that appear recent years do rely on gradient information other auxiliary knowledge, are suitable solving...
The proteolysis targeting chimera (PROTAC) strategy results in the down-regulation of unwanted protein(s) for disease treatment. In PROTAC process, a heterobifunctional degrader forms ternary complex with target protein interest (POI) and an E3 ligase, which ubiquitination proteasomal degradation POI. While formation is key attribute degraders, modification molecule to optimize can be labor-intensive tedious process. this study, we take advantage DNA-encoded library (DEL) technology...
Four cases of severe acute respiratory syndrome (SARS) that occurred from December 16, 2003, to January 8, 2004, in the city Guangzhou, Guangdong Province, China, were investigated. Clinical specimens collected these patients tested by provincial and national laboratories China as well members World Health Organization SARS Reference Verification Laboratory Network a collaborative effort identify confirm SARS-associated coronavirus (SARS-CoV) infection. Although SARS-CoV was not isolated any...
Activity monitoring, a crucial task in many applications, is often conducted expensively using video cameras. Also, effectively monitoring large field by analyzing images from multiple cameras remains challenging problem. In this paper, we introduce novel application of the recently developed RFID technology: RF tag arrays for activity where data mining techniques play critical role. The technology provides an economically attractive solution due to low cost tags and readers. Another novelty...
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority target RNAs were not or negligibly expressed. thus developed a novel strategy to achieve consistent high-yield biosynthesis chimeric carrying various small (e.g. miRNAs, siRNAs aptamers), which was based upon an optimal noncoding scaffold (OnRS) derived from tRNA fusion pre-miR-34a (tRNA/mir-34a)....
Development of anticancer treatments based on microRNA (miRNA/miR) such as miR-34a replacement therapy is limited to the use synthetic RNAs with artificial modifications. Herein, we present a new approach high-yield and large-scale biosynthesis, in <i>Escherichia coli</i> using transfer RNA (tRNA) scaffold, chimeric agent, which may act prodrug for therapy. The recombinant tRNA fusion pre–miR-34a (tRNA/mir-34a) was quickly purified high degree homogeneity (>98%) anion-exchange fast...
In contrast to the growing interests in studying noncoding RNAs (ncRNAs) such as microRNA (miRNA or miR) pharmacoepigenetics, there is a lack of efficient means cost effectively produce large quantities natural miRNA agents. Our recent efforts led successful production chimeric pre-miR-27b bacteria using transfer RNA (tRNA)–based recombinant technology, but at very low expression levels. Herein, we present high-yield pre-miR-1291 common <i>Escherichia coli</i> strains same tRNA scaffold. The...
Over the past 20 years, toolbox for discovering small-molecule therapeutic starting points has expanded considerably. Pharmaceutical researchers can now choose from technologies that, in addition to traditional high-throughput knowledge-based and diversity screening, include screening of fragment fragment-like libraries, affinity selection mass spectrometry, against DNA-encoded libraries (DELs). Each these techniques its own unique combination advantages limitations that makes them more, or...
DNA-encoded library (DEL) technology is a powerful tool for small molecule identification in drug discovery, yet the reported DEL selection strategies were applied primarily on protein targets either purified form or cellular context. To expand application of this technology, we employed an RNA target HIV-1 TAR (trans-acting responsive region), but found that majority signals resulted from false positive DNA-RNA binding. We thus developed optimized strategy utilizing patches and competitive...
We describe a novel approach for screening fragments against protein that combines the sensitivity of DNA-encoded library technology with ability to explore what will bind. Each members consists fragment which is linked photoactivatable diazirine moiety. Split and pool synthesis each set linkers version reported here containing some 70k different compounds, an individual DNA code. Incubation sample followed by photoactivation, washing subsequent PCR sequencing allows hits be identified....
As heritage is the precious treasure of human society, also carries genes culture. It vital importance to effectively develop tourism resources and explore mechanisms that influence tourists' cultural identity. This study has integrated stimulus-organism-response (SOR) framework with attitude-behavior-context (ABC) theory construct a hypothetical model aesthetics, tourist involvement, mental experience, identity so as figure out their relationships. The questionnaires were collected...
Background Hepatocellular carcinoma (HCC) continues to increase in morbidity and mortality among all types of cancer. DNA methylation, an important epigenetic modification, is associated with cancer occurrence progression. The objective this study was establish a model based on methylation risk scores for identifying new potential therapeutic targets HCC preventing Methods Transcriptomic, clinical, data 374 tumor tissues 50 adjacent normal were downloaded from Cancer Genome Atlas–Liver...