A5056137322- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Thyroid Cancer Diagnosis and Treatment
- Irrigation Practices and Water Management
- Thyroid and Parathyroid Surgery
- Crop Yield and Soil Fertility
- Climate change impacts on agriculture
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Head and Neck Anomalies
- SARS-CoV-2 and COVID-19 Research
- Identification and Quantification in Food
- Agronomic Practices and Intercropping Systems
- Rice Cultivation and Yield Improvement
- Child Nutrition and Water Access
- Voice and Speech Disorders
- Animal Virus Infections Studies
- Genomics and Phylogenetic Studies
- Greenhouse Technology and Climate Control
- Listeria monocytogenes in Food Safety
Hebei Agricultural University
2007-2025
National Institute for Communicable Disease Control and Prevention
2015-2024
Qilu Hospital of Shandong University
2015-2024
Chinese Center For Disease Control and Prevention
2004-2024
Ministry of Agriculture and Rural Affairs
2023
Lanzhou University
2023
State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems
2023
Melbourne Genomics Health Alliance
2021
South China Institute of Collaborative Innovation
2013-2019
Center for Disease Control
2004-2017
Abstract Epidemiologic investigations showed that 2 of 4 patients with severe acute respiratory syndrome (SARS) identified in the winter 2003–2004 were a waitress at restaurant Guangzhou, China, served palm civets as food and customer who ate short distance from animal cages. All 6 positive for SARS-associated coronavirus (SARS-CoV). Partial spike (S) gene sequences SARS-CoV identical to 5 S viral civets. Phylogenetic analysis was most closely related isolates. SARS cases result recent...
ABSTRACT Massive numbers of palm civets were culled to remove sources for the reemergence severe acute respiratory syndrome (SARS) in Guangdong Province, China, January 2004, following SARS coronavirus detection market animals. The virus was identified all 91 and 15 raccoon dogs animal origin sampled prior culling, but not 1,107 later at 25 farms, spread over 12 provinces, which claimed be source traded Twenty-seven novel signature variation residues (SNVs) on spike gene analyzed their...
Vibrio cholerae is the causative agent of cholera, which continues to be a major public health concern in Asia, Africa and Latin America. The bacterium can persist outside human host alternates between planktonic biofilm community lifestyles. Transition different lifestyles mediated by multiple signal transduction pathways including quorum sensing. Expression Zn-metalloprotease haemagglutinin (HA)/protease subject dual regulation involves quorum-sensing regulator HapR cAMP receptor protein....
Four cases of severe acute respiratory syndrome (SARS) that occurred from December 16, 2003, to January 8, 2004, in the city Guangzhou, Guangdong Province, China, were investigated. Clinical specimens collected these patients tested by provincial and national laboratories China as well members World Health Organization SARS Reference Verification Laboratory Network a collaborative effort identify confirm SARS-associated coronavirus (SARS-CoV) infection. Although SARS-CoV was not isolated any...
Global food security requires eco-efficient agriculture to produce the required and fiber products concomitant with ecologically efficient use of resources. This eco-efficiency concept is used diagnose state agricultural production in China (irrigated wheat-maize double-cropping systems), Zimbabwe (rainfed maize Australia wheat systems). More than 3,000 surveyed crop yields these three countries were compared against simulated grain at farmer-specified levels nitrogen (N) input. Many...
Cyclic dinucleotides act as intracellular second messengers, modulating a variety of cellular activities including innate immune activation. Although phosphodiesterases (PDEs) hydrolyzing c-di-GMP and c-di-AMP have been identified, no PDEs for cGAMPs reported. Here we identified the first three cGAMP-specific in V. cholerae (herein designated V-cGAP1/2/3). V-cGAPs are HD-GYP domain-containing proteins specifically break 3′3′-cGAMP, but not other forms cGAMP. 3′3′-cGAMP is linearized by all...
IEM101, a Vibrio cholerae O1 El Tor Ogawa strain naturally deficient in CTXPhi, was previously selected as live cholera vaccine candidate. To make better and safer that can induce protective immunity against both the bacteria toxin (CT), new candidate, IEM108, constructed by introducing ctxB gene an Tor-derived rstR into IEM101. The codes for antigen CTB subunit, mediates phage immunity. stable expression of two genes managed chromosome-plasmid lethal balanced system based on housekeeping...
Although 5-fluorouracil (5-FU) is the primary chemotherapy treatment for colorectal cancer (CRC), its efficacy limited by drug resistance. Ferroptosis activation a promising 5-FU-resistant cells; however, potential therapeutic targets remain elusive. This study investigated ferroptosis vulnerability and dihydroorotate dehydrogenase (DHODH) activity using stable, CRC cell lines xenograft models. was characterized measuring malondialdehyde levels, assessing lipid metabolism peroxidation,...
Vibrio cholerae can enter into a viable but non-culturable (VBNC) state in order to survive unfavorable environments. In this study, we studied the roles of five physicochemical and microbiological factors or states, namely, different strains, growth phases, oxygen, temperature, starvation, on development VBNC V. artificial sea water (ASW). Different strains organism, phase, oxygen levels affected progress development. It was found that induced faster serogroup O1 classical biotype strain...
There are increasing concerns about the negative effects of conventional tillage (CT), extreme climate events, increased weed incidence, and reduced availability farm inputs on crop yield stability, profitability, water productivity in rice-wheat (R−W) systems Eastern Gangetic Plains (EGP) South Asia. Conservation agriculture (CA)-based practices have been proposed region to address these concerns. We analysed 84 on-farm trials' data comparing CA-based with farmers' CT R−W eastern Terai...
Inactivation of the quorum-sensing regulator HapR causes Vibrio cholerae El Tor biotype strain C7258 to adopt a rugose colonial morphology that correlates with enhanced biofilm formation. V. mutants lacking cyclic AMP (cAMP) receptor protein (CRP) produce very little HapR, which results in elevated expression exopolysaccharide (vps) genes and compared wild type. However, Deltacrp still exhibited smooth expressed reduced levels vps isogenic hapR mutants. In this study we demonstrate deletion...
Vibrio fluvialis is considered to be an emerging foodborne pathogen and has been becoming a high human public health hazard all over the world, especially in coastal areas of developing countries regions with poor sanitation. The distribution virulence factors, microbiological molecular epidemiological features V. isolates China remains examined.PCR targeted at determinants phenotype tests including metabolism, antibiotic susceptibility were performed. Pulsed-field gel electrophoresis (PFGE)...
Abstract TfoX (Sxy) and CRP are two important competence activators. The link between tfoX has been shown in H. influenza but lacking evidence of direct interaction. Recently a Sxy-dependent (CRP-S) site autoregulating Sxy was reported E. coli . Here, we show that the cAMP-CRP complex transcriptionally regulates expression through multiple canonical (CRP-N) sites Vibrios. This conclusion is supported by an analysis mRNA levels transcriptional reporter fusions. reduced VC restored...
Vibrio fluvialis is an emerging foodborne pathogen of increasing public health concern. The mechanism(s) that contribute to the bacterial survival and disease are still poorly understood. In other species, type VI secretion systems (T6SSs) known pathogenicity by exerting toxic effects on host cells or competing species. this study, we characterized genetic organization prevalence two T6SS gene clusters (VflT6SS1 VflT6SS2) in V. fluvialis. VflT6SS2 harbors three "orphan" hcp-vgrG modules was...
Despite recent evidence, the role of human papillomavirus (HPV) in breast carcinogenesis is controversial. The correlations HPV infection with clinicopathological features cancer and expression cell cycle/apoptosis-associated proteins have not been well elucidated. In this study, we sought to determine prevalence high-risk HPVs (HR-HPVs) BCL2, p21, p53, Rb, survivin patients investigate relationship these cancer-related proteins, an attempt clarify potential mechanism pathogenesis. presence...
Summary Conservation agriculture (CA), as a key component of sustainable intensification, has been widely promoted across sub-Saharan Africa (SSA) to address low crop productivity. However, the focus mainly on improving cereal grain yields, with less its impact nutritional outcomes. This study sought assess productivity potential CA diversification systems and associated establishment techniques in terms grain, protein, energy yields. An on-station trial was implemented Malawi for four...
Introduction: This study was conducted to determine the prevalence of serotypes, virulence factors, and antimicrobial resistance patterns Shigella spp. in Beijing, China, from 2004 2011. Methodology: Real-time PCR assays were used detect virulent genes, Kirby-Bauer disk diffusion method evaluate resistance. Results: Among total 1,652 isolates, S. sonnei (57.1%) predominant species, followed by flexneri (42.3%), dysenteriae (0.4%), boydii (0.2%). Nineteen serotypes discovered among strains....
ABSTRACT Production of the Zn-metalloprotease hemagglutinin (HA)/protease by Vibrio cholerae has been reported to enhance enterotoxicity in rabbit ileal loops and reactogenicity live cholera vaccine candidates. Expression HA/protease requires alternate sigma factor σ S (RpoS), encoded rpoS . The histone-like nucleoid structuring protein (H-NS) shown repress expression Escherichia coli In V. strains classical biotype, H-NS silence virulence gene expression. this study we examined role...
Abstract The etiology of breast cancer remains unknown and the role human papillomavirus (HPV) in carcinogenesis is controversial. This study investigated prevalence high‐risk HPV infections Chinese women with possible relationship between infection clinicopathological characteristics cancer. Tumor cells from 224 fresh samples 37 fibroadenomas were collected for hybrid capture 2 (HC2) assay. HC2 was only technique approved by United States Food Drug Administration screening 2008. 21.4%,...
Vibrio fluvialis, an emerging foodborne pathogen of increasing public health concern, contains two distinct gene clusters encoding type VI secretion system (T6SS), the most newly discovered pathway in Gram-negative bacteria. Previously we have shown that one T6SS clusters, namely VflT6SS2, is active and associates with anti-bacterial activity. However, how its activity regulated not completely understood. Here, report global regulator integration host factor (IHF) positively modulates...