- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Pancreatitis Pathology and Treatment
- Insect-Plant Interactions and Control
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Liver Disease Diagnosis and Treatment
- Plant Gene Expression Analysis
- Hepatitis B Virus Studies
- Ubiquitin and proteasome pathways
- Pancreatic and Hepatic Oncology Research
- Transgenic Plants and Applications
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Soybean genetics and cultivation
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
- Plant Pathogenic Bacteria Studies
First Affiliated Hospital of Xi'an Jiaotong University
2013-2025
Xi'an Jiaotong University
2013-2025
Zhoukou Normal University
2016-2025
Nanchang University
2020-2024
Second Affiliated Hospital of Nanchang University
2020-2024
Henan Agricultural University
2019
China Agricultural University
2014
Liaocheng University
2014
Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used transient to follow the vivo behavior of proteins. Onion epidermis, which has large, living transparent cells a monolayer, suitable visualize fluorescent The methods included particle bombardment, protoplast transfection Agrobacterium-mediated Particle bombardment onion epidermis was successfully established,...
Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be monocots, by presenting a system involving use of novel infiltration solution (containing acetosyringone, cysteine, and Tween 20) enables whole-plant level VIGS (germinated) seeds wheat maize. Using the established system, phytoene desaturase (PDS) genes were successfully silenced, resulting typical photo-bleaching symptoms leaves treated In addition,...
Objectives: Hypertriglyceridemia-associated acute pancreatitis (HTG-AP) is one of the most common etiologies (AP) worldwide. Compared to other etiologies, patients with HTG-AP may develop more severe AP, but previous studies yielded controversial conclusion due lack adequate adjustment for confounders. Therefore, this study aimed examine possibility and risk factors developing AP in HTG-AP. Methods: Data from an established diagnosis were collected January 2013 December 2023 using a...
Tomato Verticillium wilt is a soil-borne vascular disease caused by the necrotrophic fungus dahliae. Although some understanding of plant defense mechanisms against V. dahliae infection has been gained for incompatible interactions, including identification inducible resistant genes and signaling pathways, pathways involved in compatible interaction remain unclear. To investigate molecular basis between tomato dahliae, transcriptomes infected tomatoes were compared to those control group. A...
Transcription factors of the AP2/ERF family play important roles in plant growth, development, and responses to biotic abiotic stresses. In this study, a physic nut gene, JcDREB2, was functionally characterized. Real-time PCR analysis revealed that JcDREB2 expressed mainly leaf could be induced by abscisic acid (ABA) but suppressed gibberellin (GA) salt. Transient expression JcDREB2-YFP fusion protein Arabidopsis protoplasts cells suggested is localized nucleus. Rice plants overexpressing...
Abstract Whitefly-transmitted begomoviruses are economically important plant pathogens that cause severe problems in many crop plants, such as tomato, papaya, cotton, and tobacco. Tomato yellow leaf curl virus (TYLCV) is a typical monopartite begomovirus has been extensively studied, but methods can efficiently control still scarce. In this study, we combined artificial microRNA (amiRNA)-mediated silencing technology clay nanosheet-mediated delivery by spraying developed method for...
The bean bug, Riptortus pedestris, has received intense attention in recent years because of its involvement increasing outbreaks staygreen syndrome soybean (Glycine max (L.)), often causing almost 100% loss yield China. However, for this pest great economic importance, potential current and future distribution patterns their underlying driving factors remain unclear.Maxent modelling under climate, elevation land-use (including the information G. max) variables showed that covered a vast...
This study aimed to investigate the effects of multiwalled carbon nanotubes (MWNTs) co-delivering sorafenib (Sor) and epidermal growth factor receptor (EGFR) siRNA (MWNT/Sor/siRNA) on tumor in liver cancer (LC).
Phosphorus (P) deficiency is one of the main growth-limiting factors for plants. However, arbuscular mycorrhizal (AM) symbiosis can significantly promote P uptake. Generally, PHT1 transporters play key roles in plants' uptake, and thus, genes have been investigated some plants, but regulation functions these wheat (TaPHT1) during AM not studied depth. Therefore, a comprehensive analysis TaPHT1 was performed, including sequence, phylogeny, cis-elements, expression, subcellular localization...
The HD-ZIP protein, a unique class of transcription factors in plants, plays crucial role plant growth and development. Although some have been associated with leafy head formation Chinese cabbage, their regulatory mechanisms remain poorly understood. This study identified the family using HMM TBtools, constructed phylogenetic tree OrthoFinder, analyzed gene expansion contraction CAFE. Conserved features were MAFFT, MEME, TBtools; networks predicted ATRM PlantTFDB; expression was validated...
Purpose: This study aims to analyze the risk factors influencing survival and prognosis of patients with acute pancreatitis (AP) complicated by kidney injury (AKI) develop a nomogram model for predicting overall (OS) these patients. Patients methods: 616 diagnosed AP sourced from MIMIC-IV database. They were randomly divided into training set an internal validation set. Another 60 First Affiliated Hospital Xi’an Jiaotong University used as external Univariate multivariate Cox regression...
BABY BOOM (BBM), initially identified in Brassica napus, can enhance the shoot regeneration capacity tissue culture and is involved conversion from vegetative to embryogenic state. This study aimed isolate BBM orthologue genes Rosa canina analyse their functions. Two full-length cDNAs, designated RcBBM1 RcBBM2, were isolated R. by rapid amplification of cDNA ends (RACE). The predicted amino acid sequences two RcBBMs contained bbm-1 motif motifs typically conserved eudicotANT (euANT) lineage....
A new protocol was established for the regeneration of Solanum nigrum by frog egg-like bodies (FELBs), which are novel somatic embryogenesis (SE) structures induced from root, stem, and leaf explants. The explants (93.33%, 85.10%, 100.00%, respectively) were to form special embryonic calli on Murashige Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid, under dark condition. Further, (86.97%, 83.30%, 99.47%, developed into FELBs. Plantlets FELBs three in vitro MS...
Abstract An optimized regeneration and Agrobacterium ‐mediated transformation protocol based on whole cotyledonary node explants was developed in soybean ( Glycine max ) cultivar Zhong Huang 13. Adding 6‐benzylaminopurine (BAP) a germinating medium could significantly increase efficiency; the optimal BAP concentration for shoot formation 0.5 mg/L. The concentrations of plant growth regulators induction were by orthogonal test [L 9 (3 3 )]. best combination Murashige & Skoog salts with B5...