A5034110656- Bacterial Identification and Susceptibility Testing
- Toxoplasma gondii Research Studies
- Antimicrobial Resistance in Staphylococcus
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Infective Endocarditis Diagnosis and Management
- Autophagy in Disease and Therapy
- Sinusitis and nasal conditions
- Pharmaceutical and Antibiotic Environmental Impacts
- Reproductive System and Pregnancy
- SARS-CoV-2 detection and testing
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Antimicrobial Peptides and Activities
- Oral microbiology and periodontitis research
- Neurogenesis and neuroplasticity mechanisms
- Ocular Infections and Treatments
- Enterobacteriaceae and Cronobacter Research
- Urinary Tract Infections Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Streptococcal Infections and Treatments
- Psychodrama and Leishmaniasis Studies
College of Staten Island
2023
City University of New York
2023
Massachusetts Eye and Ear Infirmary
2016-2020
Harvard University
2016-2020
Washington University in St. Louis
2012-2015
Institute of Cellular and Intracellular Symbiosis
2010
Soil microbiota represent one of the ancient evolutionary origins antibiotic resistance and have been proposed as a reservoir genes available for exchange with clinical pathogens. Using high-throughput functional metagenomic approach in conjunction pipeline de novo assembly short-read sequence data from selections (termed PARFuMS), we provide evidence recent between environmental bacteria We describe multidrug-resistant soil containing cassettes against five classes antibiotics (β-lactams,...
IFN-γ activates cells to restrict intracellular pathogens by upregulating cellular effectors including the p65 family of guanylate-binding proteins (GBPs). Here we test role Gbp1 in IFN-γ-dependent control T. gondii mouse model. Virulent strains avoided recruitment parasitophorous vacuole a strain-dependent manner that was mediated parasite virulence factors ROP18, an active serine/threonine kinase, and pseudokinase ROP5. Increased Δrop18 or Δrop5 parasites associated with clearance...
ABSTRACT A core set of autophagy proteins is required for gamma interferon (IFN-γ)-mediated clearance Toxoplasma gondii in the mouse because their control several downstream effectors, including immunity-related GTPases (IRGs) and guanylate-binding (GBPs). However, these effectors are absent (i.e., IRGs) from or nonessential GBPs) IFN-γ-activated human cells, raising question how cells parasite replication. Here, we define a novel role ubiquitination recruitment adaptors strain-specific T....
During infection with the intracellular parasite Toxoplasma gondii, presentation of parasite-derived antigens to CD4+ and CD8+ T cells is essential for long-term resistance this pathogen. Fundamental questions remain regarding roles phagocytosis active invasion in events that lead processing antigens. To understand most proximal process, an attenuated non-replicating strain T. gondii (the cpsII strain) was combined a cytometry-based approach distinguish from phagocytic uptake. In vivo...
Chlamydia trachomatis is a human pathogen of global importance. An obstacle to studying the pathophysiology chlamydial disease lack suitable murine model C. infection. Mice are less susceptible infection with isolates due in part innate mouse-specific host defense mechanisms which strains sensitive. Another possible factor that influences susceptibility mice commonly cultivated vitro prior mice; therefore, virulence genes could be lost as consequence negative selective pressure. We tested...
Lipopeptide daptomycin is a last-line cell-membrane-targeting antibiotic to treat multidrug-resistant Staphylococcus aureus Alarmingly, daptomycin-resistant S. isolates have emerged. The mechanisms underlying resistance are diverse and share similarities with resistances cationic antimicrobial peptides other lipopeptides, but they remain be fully elucidated. We selected mutants increased from library of transposon insertions in sequent type 8 (ST8) HG003. Insertions conferring were localized...
The enterococci, which are among the leading causes of multidrug-resistant (MDR) hospital infection, notable for their environmental ruggedness, extends to intrinsic antibiotic resistance. To identify genes that confer this unique property, we used Tn-seq comprehensively explore genome MDR Enterococcus faecalis strain MMH594 important growth in nutrient-containing medium and with low-level challenge. As expected, a large core DNA replication, expression, central metabolism, shared other...
Abstract Oligodendrocyte progenitor cells (OPCs) receive synaptic innervation from glutamatergic and GABAergic axons can be dynamically regulated by neural activity, resulting in activity-dependent changes patterns of axon myelination. However, it remains unclear to what extent other types neurons may innervate OPCs. Here, we provide evidence implicating midbrain dopamine the oligodendrocyte lineage anterior corpus callosum nearby white matter tracts male female adult mice. Dopaminergic...
The ability to control the spread of COVID-19 continues be hampered by a lack rapid, scalable, and easily deployable diagnostic solutions.We developed method based on CRISPR (clustered regularly interspaced short palindromic repeats) that can deliver sensitive, specific, high-throughput detection Sudden Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2). assay utilizes SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) for qualitative SARS-CoV-2 RNA may performed directly...