A5041583598- Ultrasound and Hyperthermia Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Photoacoustic and Ultrasonic Imaging
- Anesthesia and Neurotoxicity Research
- Coronary Interventions and Diagnostics
- Nanoplatforms for cancer theranostics
- Electrospun Nanofibers in Biomedical Applications
- Exercise and Physiological Responses
- Cardiovascular and exercise physiology
- Angiogenesis and VEGF in Cancer
- Intracranial Aneurysms: Treatment and Complications
- Muscle activation and electromyography studies
- Acute Ischemic Stroke Management
- Neuroscience and Neuropharmacology Research
- Cerebrovascular and Carotid Artery Diseases
- Nitric Oxide and Endothelin Effects
- RNA Interference and Gene Delivery
- Pulmonary Hypertension Research and Treatments
- Peripheral Artery Disease Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular and Diving-Related Complications
- Tissue Engineering and Regenerative Medicine
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
The University of Texas Health Science Center at Houston
2014-2024
University of Cincinnati
2009-2023
Boston Scientific (United States)
2022
Texas A&M University
2009
Texas College
2009
University of Houston
2007
Baylor College of Medicine
1992-2000
The University of Texas Health Science Center at San Antonio
1992
University of California, Los Angeles
1956
We have tested the hypothesis that diaphragm muscle fibers release superoxide anion radicals (O2-.) into extracellular space. Fiber bundles were isolated from rat and incubated in Krebs-Ringer solution containing cytochrome c (10(-5) M), a standard assay for O2-.. Bundles either passive or active, i.e., directly stimulated to contract rhythmically. After 1 h, absorbance of reduced incubation medium was measured at 550 nm. Absorbance greater exposed than without (P < 0.01), indicating O2-....
This study tested the hypothesis that reactive oxygen intermediates present in unfatigued skeletal muscle act to enhance contractile function. Fiber bundles from rat diaphragm were incubated with exogenous catalase (an antioxidant enzyme dehydrates hydrogen peroxide molecular and water) decrease tissue concentration of intermediates. Catalase (10(3) U/ml) significantly decreased twitch characteristics (time peak tension, half-relaxation time, force, twitch-to-tetanus force ratio), thereby...
Background —We have developed a transgenic mouse with cardiac-restricted overexpression of tumor necrosis factor-α (TNF-α). These mice develop heart failure phenotype characterized by left ventricular dysfunction and remodeling, pulmonary edema, elevated levels TNF-α in the peripheral circulation from cardiac spillover. Given that causes atrophy loss function respiratory muscle, we asked whether diaphragm contractile losses were caused oxidative stress or tissue remodeling. Methods Results...
The goal of this study was to evaluate the therapeutic window for hypothermia treatment following experimental brain injury by measuring edema formation and functional outcome.Traumatic (TBI) produced in anesthetized rats using cortical impact injury. Edema measured ipsilateral contralateral hemispheres subtracting dry weight from wet weight, neurological function assessed a battery behavioral tests 24 hours after TBI. In injured rats, it found that water levels were elevated at I hour...
Xenon (Xe), a noble gas, has promising neuroprotective properties with no proven adverse side-effects. We evaluated effects of Xe delivered by Xe-containing echogenic liposomes (Xe-ELIP) via ultrasound-controlled cerebral drug release on early brain injury following subarachnoid hemorrhage (SAH). The Xe-ELIP structure was ultrasound imaging, electron microscopy and gas chromatography-mass spectroscopy. Animals were randomly divided into five groups: Sham, SAH, SAH treated Xe-ELIP, empty...
Skeletal muscle constitutively expresses both the type I (neuronal) and III (endothelial) isoforms of nitric oxide synthase (NOS). We tested functional importance NOS using skeletal muscles with similar levels expression (diaphragm soleus) from wild-type, heterozygous, NOS-deficient littermate mice. Muscles were incubated at 37°C in Krebs-Ringer solution. NO accumulation medium was measured by chemiluminescence; force-frequency fatigue characteristics direct electrical stimulation. Diaphragm...
Dimethyl sulfoxide (DMSO) is commonly used in studies of skeletal muscle as a selective antioxidant (DMSO preferentially scavenges hydroxyl radicals) or solvent for drugs. The present experiments tested DMSO direct effects on diaphragm contractile properties. Fiber bundles were removed from anesthetized rats, mounted vitro at optimal length (37 degrees C), curarized, and stimulated directly. Protocol 1 depression dose dependence by comparing treated with (0.6–640 mM) time- stimulus-matched...
In atherosclerosis, the loss of vascular stem cells via apoptosis impairs capacity wall to repair or regenerate tissue damaged by atherogenic factors. Recruitment exogenous plaque may repopulate and help arterial tissue. Ultrasound-enhanced liposomal targeting provide a feasible method for cell delivery into atheroma. Bifunctional echogenic immunoliposomes (BF-ELIP) were generated covalently coupling two antibodies liposomes; first one specific CD34 antigens on surface second directed...
Objective— Ultrasound enhances thrombolysis when combined with a thrombolytic and contrast agent. This study aimed to evaluate the effect of our tissue plasminogen activator (tPA)–loaded echogenic liposomes (ELIP) in an vivo clot model, without ultrasound treatment. Methods Results— The femoral arteries New Zealand White rabbits (n=4 per group) were cannulated. abdominal aortas denuded, thrombi created using solution sodium ricinoleate plus thrombin. Rabbits then randomly selected receive...
Xenon (Xe) has shown great potential as a stroke treatment due to its exceptional ability protect brain tissue without inducing side effects. We have previously developed Xe-loaded liposomes for the ultrasound-activated delivery of Xe into cerebral region and demonstrated their therapeutic efficacy. At present, sole FDA-approved thrombolytic agent is recombinant plasminogen activator (rtPA). In this study, we aimed investigate combining Xe-liposomes with an intravenous rtPA in clinically...
It has been demonstrated that ethanol exerts dose-dependent effects, both beneficial and detrimental, on the outcome of traumatic brain injury (TBI). Recently, it reported co-administration caffeine (10 mg/kg) a low amount alcohol (0.65 g/kg; caffeinol) reduces cortical infarct volume up to 80%, improves motor coordination, following rodent model reversible common carotid/middle cerebral artery occlusion. However, protective effects caffeinol other CNS insults, nor its influence cognitive...
This study aimed to demonstrate three-dimensional (3D) visualization of early/inflammatory arterial atheroma using intravascular ultrasound (IVUS) and targeted echogenic immunoliposomes (ELIP). IVUS can be used as a molecular imaging modality with the use contrast agents for detection. Three-dimensional reconstruction 2-dimensional images may provide improved visualization.Atheroma were induced in arteries Yucatan miniswine (n = 5) by endothelial cell denudation followed 4-week high...
Liposomes as carriers for CRISPR/Cas9 complexes represent an attractive approach cardiovascular gene therapy. A critical barrier to this remains the efficient delivery of CRISPR-based genetic materials into cardiomyocytes. Echogenic liposomes (ELIP) containing a fluorescein isothiocyanate-labeled decoy oligodeoxynucleotide against nuclear factor kappa B (ELIP-NF-κB-FITC) were used both in vitro on mouse neonatal ventricular myocytes and vivo rat hearts assess efficacy with or without...
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CT studies of the abdomen performed on 72 patients with small-cell carcinoma lung were retrospectively reviewed to assess role abdominal in staging. Forty-four had extensive disease, defined as disease extending beyond confines one hemithorax, plus or minus mediastinal ipsilateral supraclavicular pleural effusion. Initial-staging revealed more sites metastatic 26 (59%) these 44 patients, while 18 normal initial examinations. Statistical analysis a significant increase complete therapeutic...
Late in-stent restenosis remains a significant problem. Bare-metal stents were implanted into peripheral arteries in miniature swine, followed by direct intra-arterial infusion of nitric oxide–loaded echogenic liposomes (ELIPs) and anti–intercellular adhesion molecule-1 conjugated ELIPs loaded with pioglitazone exposed to an endovascular catheter ultrasonic core. Ultrasound-facilitated delivery ELIP formulations stented attenuated neointimal growth. Local atheroma-targeted,...
Rationale We have produced a liposomal formulation of xenon (Xe‐ELIP) as neuroprotectant for inhibition brain damage in stroke patients. This mandates development reliable assay to measure the amount dissolved released from Xe‐ELIP water and blood samples. Methods Gas chromatography/mass spectrometry (GC/MS) was used quantify gas into headspace vials containing samples or blood. In order determine concentration vivo after administration, 6 mg lipid infused intravenously rats. Blood were...
Fifteen rats with severed spinal cords, kept alive on a respirator, were given massive doses of epinephrine, that controls produced consistently an intense acute pulmonary edema. None these developed the usual edema, and in 10 cases lungs had normal weight. This result is interpreted as proof central nervous mediation edema by administration epinephrine.