A5090290793- Prion Diseases and Protein Misfolding
- Helminth infection and control
- Coccidia and coccidiosis research
- Forensic Entomology and Diptera Studies
- Forest Ecology and Biodiversity Studies
- Insect Utilization and Effects
- Immunotherapy and Immune Responses
- Parasite Biology and Host Interactions
- Neurological diseases and metabolism
- Monoclonal and Polyclonal Antibodies Research
- Trace Elements in Health
- Parasites and Host Interactions
- Animal Virus Infections Studies
- Amino Acid Enzymes and Metabolism
- Glycosylation and Glycoproteins Research
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Chemokine receptors and signaling
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Veterinary medicine and infectious diseases
- Pharmacogenetics and Drug Metabolism
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Animal Nutrition and Physiology
Virologie et Immunologie Moléculaires
2014-2024
Université de Versailles Saint-Quentin-en-Yvelines
2023-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2024
Université Paris-Saclay
2016-2024
Institut de Biologie Intégrative de la Cellule
2023
Institut National de la Recherche Agronomique
2000-2013
Infectiologie Animale et Santé Publique
2006-2012
Biologie des Oiseaux et Aviculture
2000-2005
Institut Cochin
1995-1997
Centre National de la Recherche Scientifique
1995-1997
Prions are infectious pathogens essentially composed of PrP(Sc), an abnormally folded form the host-encoded prion protein PrP(C). Constrained steric interactions between PrP(Sc) and PrP(C) thought to provide prions with species specificity control cross-species transmission into other host populations, including humans. We compared ability brain lymphoid tissues from ovine human PrP transgenic mice replicate foreign, inefficiently transmitted prions. Lymphoid tissue was consistently more...
ABSTRACT An enzyme-linked lectinsorbent assay (ELLA) was developed for quantification and characterization of extracellular polysaccharides produced by 1- 4-day biofilms 10 bacterial strains isolated from food industry premises. Peroxidase-labeled concanavalin A (ConA) wheat germ agglutinin (WGA) were used, as they specifically bind to saccharide residues most frequently encountered in matrices: d -glucose or -mannose ConA N -acetyl- -glucosamine -acetylneuraminic acid WGA. The ELLA applied...
Prions are proteinaceous infectious agents responsible for fatal neurodegenerative diseases in animals and humans. They essentially composed of PrP(Sc), an aggregated, misfolded conformer the ubiquitously expressed host-encoded prion protein (PrP(C)). Stable variations PrP(Sc) conformation assumed to encode phenotypically tangible strains diversity. However direct contribution quaternary structure strain biological information remains mostly unknown. Applying a sedimentation velocity...
Measurements of the presence prions in biological tissues or fluids rely more and on cell-free assays. Although protein misfolding cyclic amplification (PMCA) has emerged as a valuable, sensitive tool, it is currently hampered by its lack robustness rapidity for high-throughput purposes. Here, we made number improvements making possible to amplify maximum levels scrapie single 48-h round microplate format. The rates infectious titer PMCA-formed appeared similar those derived from vivo...
The existence of conventional dendritic cells (cDCs) has not yet been demonstrated outside mammals. In this article, we identified bona fide cDCs in chicken spleen. Comparative profiling global and immune response gene expression, morphology, T cell activation properties show that macrophages (MPs) exist as distinct mononuclear phagocytes the chicken, resembling their human mouse counterparts. With computational analysis, core expression signatures for cDCs, MPs, B across human, were...
Abstract Three monoclonal IgG2a anti‐DNA polyreactive autoantibodies, derived from lupus‐prone mice (NZB X NZW)F1, were studied by surface plasmon resonance (BIAcore) analysis using three different synthetic double‐stranded (ds) oligonucleotides of 25, 30, and 25 base pairs (bp). These antibodies (mAb) exhibited dissociation rate constants (k off ) ranging 0.0001 (mAb F14.6 F4.1) to 0.01/s J20.8) k on 2 × 10 5 6 /M/s. The screening a constrained random peptide library displayed M13...
Abstract Prions are formed of misfolded assemblies (PrP Sc ) the variably N-glycosylated cellular prion protein C ). In infected species, prions replicate by seeding conversion and polymerization host PrP . Distinct strains can be recognized, exhibiting defined biochemical properties such as glycotype specific biological traits. While strain information is encoded within conformation assemblies, storage structural molecular requirements for self-perpetuation remain uncertain. Here, we...
Prion diseases are characterized by conformational changes of a cellular prion protein (PrP(C)) into β-sheet-enriched and aggregated conformer (PrP(Sc)). Shadoo (Sho), member the family, is expressed in central nervous system (CNS) highly conserved among vertebrates. On basis histoanatomical colocalization sequence similarities, it suspected that Sho PrP may be functionally related. The downregulation expression during pathology direct interaction between PrP, as revealed two-hybrid...
Human RSV is the leading cause of infantile bronchiolitis in world and one major causes childhood deaths resource-poor settings. It a unmet target for vaccines anti-viral drugs. Respiratory syncytial virus has evolved unique strategy to evade host immune response by coding two non-structural proteins NS1 NS2. Recently it was shown that infected cells, nuclear could be involved transcription regulation genes linked innate response, via interactions with chromatin Mediator complex. Here we...
The distribution of lymphocyte subpopulations (TCD4+, TCD8+, TCD43+ and Ig+ cells), macrophages eosinophils were analysed in the inflammatory infiltrates associated with hepatic lesions lymph nodes (HLN) from rats experimentally infected F. hepatica necropsied 1, 2, 3, 4, 6 8 week post infection (WPI). We also investigated fixation immunoglobulin isotypes on migrating flukes liver. As early as 1WPI, portal tract areas surrounding migratory tunnels infiltrated immune cells. dominant cells to...
Aims: Chronic wasting disease (CWD) in cervids and BSE cattle are prion diseases that negatively affect economy, ecology as well animal possibly human health Canada elsewhere.CWD incidence is reaching 15% mule deer Alberta hunting areas doing testing, a tenfold increase over the last 10 years.Caribou still free of CWD, but transmission into caribou will happen soon.Caribou major food source for Native Northern populations, so security safety at risk.We described genetic factors protein (PrP)...
Abstract In peripherally acquired prion diseases, prions move through several tissues of the infected host, notably in lymphoid tissue, long before occurrence neuroinvasion. Accumulation can even be restricted to tissue without neuroinvasion and clinical disease. Several experimental observations indicated that presence differentiated follicular dendritic cells (FDCs) structures strain type are critical determinants extraneural replication. this context, report granulomatous apparently...
Summary Prion diseases, or Transmissible Spongiform Encephalopathies (TSE), are neurodegenerative disorders caused by the accumulation of misfolded conformers (PrP Sc ) cellular prion protein C ). During pathogenesis, PrP seeds disseminate in central nervous system and convert leading to formation insoluble assemblies. As for conventional infectious variations clinical manifestation define a specific strain which correspond different structures. In this work, we implemented recent...