A5101880571- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Mycorrhizal Fungi and Plant Interactions
- Agronomic Practices and Intercropping Systems
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Coastal wetland ecosystem dynamics
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Lysosomal Storage Disorders Research
- Ion Channels and Receptors
- Marine and fisheries research
- Virus-based gene therapy research
- Coral and Marine Ecosystems Studies
- Synthesis and Biological Evaluation
- Ion channel regulation and function
- Respiratory and Cough-Related Research
- Genetic Associations and Epidemiology
- Advanced Measurement and Detection Methods
- Protein Tyrosine Phosphatases
- Bauxite Residue and Utilization
- Hedgehog Signaling Pathway Studies
Kwansei Gakuin University
2017-2024
National Institute for Basic Biology
2012-2022
Kobe Gakuin University
2016-2021
The Graduate University for Advanced Studies, SOKENDAI
2012-2017
Ludwig-Maximilians-Universität München
2006-2013
Institute of Agrobiological Sciences
2008-2012
University of Tsukuba
2009
Nagoya University
2007-2009
Kazusa DNA Research Institute
2009
John Innes Centre
2009
The anterior part of the vertebrate head expresses a group homeo box genes in segmentally restricted patterns during embryogenesis. Among these, Otx2 expression covers entire fore- and midbrains takes place earliest. To examine its role development rostral head, mutation was introduced into this locus. homozygous mutants did not develop structures to rhombomere 3, indicating an essential formation head. In contrast, heterozygous displayed craniofacial malformations designated as otocephaly;...
Gangliosides, sialic acid-containing glycosphingolipids, are abundant in the vertebrate (mammalian) nervous system. Their composition is spatially and developmentally regulated, gangliosides have been widely believed to lay essential roles establishment of system, especially neuritogenesis synaptogenesis. However, this has never tested directly. Here we report generation mice with a disrupted beta 1,4-N-acetylgalactosaminyltransferase (GM2/GD2 synthase; EC 2.4.1.92) gene. The lacked all...
In attempting to produce a mutant mouse with embryonic stem cells, the critical step is efficient isolation of homologous recombinants; frequency recombination usually low and potency cells differentiate into germ unstable in culture. Here, we report an efficacious method for such which diphtheria toxin A-fragment gene used negatively select nonhomologous recombinants. contrast use herpes simplex virus thymidine kinase gene, selection can be made singly by neomycin analog G418 without using...
The mechanism underlying perinuclear calcium spiking induced during legume root endosymbioses is largely unknown. Lotus japonicus symbiosis-defective castor and pollux mutants are impaired in spiking. Homology modeling suggested that the related proteins CASTOR POLLUX might be ion channels. Here, we show form two independent homocomplexes planta. reconstituted planar lipid bilayers exhibited channel activity, characteristics were altered a mutant carrying an amino acid replacement close to...
Abstract Legumes form symbioses with arbuscular mycorrhiza (AM) fungi and nitrogen fixing root nodule bacteria. Intracellular infection by either endosymbiont is controlled the activation of calcium calmodulin-dependent kinase (CCaMK), a central regulatory component plant's common symbiosis signaling network. We performed microscopy screen for Lotus japonicus mutants defective in AM development isolated mutant, nena, that aborted fungal rhizodermis. NENA encodes WD40 repeat protein related...
Root nodule formation is regulated by several plant hormones, but the details of regulation nodulation signaling pathway are largely unknown. In this study, role gibberellin (GA) in control root symbiosis was investigated at physiological and genetic levels Lotus japonicus. Exogenous application biologically active GA, GA(3), inhibited infection threads nodules, which counteracted a biosynthesis inhibitor Uniconazole P. Nod factor-induced hair deformation severely blocked presence...
The interaction of legumes with N2-fixing bacteria collectively called rhizobia results in root nodule development. number nodules formed is tightly restricted through the systemic negative feedback control by host autoregulation nodulation (AON). Here, we report characterization and gene identification TOO MUCH LOVE (TML), a factor that acts during AON model legume Lotus japonicus. In our genetic analyses using another root-regulated hypernodulation mutant, plenty, tml-1 plenty double...
Arbuscular mycorrhizal (AM) symbiosis is the most widespread association between plants and fungi. To provide novel insights into molecular mechanisms of AM symbiosis, we screened investigated genes fungus Rhizophagus irregularis that contribute to infection host plants. R. involved in were explored by RNA-sequencing (RNA-seq) analysis. One identified was then characterized a reverse genetic approach using host-induced gene silencing (HIGS), which causes RNA interference via plant. The...
Gene expression during arbuscular mycorrhizal development is highly orchestrated in both plants and fungi. To elucidate the gene profiles of symbiotic association, we performed a digital analysis Lotus japonicus Rhizophagus irregularis using HiSeq 2000 next-generation sequencer with Cufflinks assembly de novo transcriptome assembly. There were 3,641 genes differentially expressed L. japonicus, approximately 80% which up-regulated. The up-regulated included secreted proteins, transporters,...
Abstract Arbuscular mycorrhiza is a mutualistic plant-fungus interaction that confers great advantages for plant growth. mycorrhizal (AM) fungi enter the host root and form symbiotic structures facilitate nutrient supplies between symbionts. The gibberellins (GAs) are phytohormones known to inhibit AM fungal infection. However, our transcriptome analysis phytohormone quantification revealed GA accumulation in roots of Lotus japonicus infected with fungi, suggesting de novo synthesis plays...
Summary The rice lysin‐motif (LysM) receptor‐like kinase Os CERK 1 is now known to have a dual role in both pathogenic and symbiotic interactions. Following the recent discovery that Oscerk1 mutant unable host arbuscular mycorrhizal ( AM ) fungi, we examined whether directly involved perception of short‐chain chitin oligomers (Myc‐ CO s) identified fungal exudates shown activate nuclear calcium (Ca 2+ spiking root epidermis. An knockout expressing cameleon NLS ‐ YC 2.60 was used monitor Ca...
In the arbuscular mycorrhiza (AM) symbiosis, plant roots accommodate Glomeromycota fungi within an intracellular compartment, arbuscule. At this symbiotic interface, fungal hyphae are surrounded by a membrane, which creates apoplastic periarbuscular space (PAS) between and cell. Despite importance of PAS for signal metabolite exchange, only few its components have been identified. Here we show that two proteases subtilase family required AM development. SbtM1 is founder member...
The common symbiosis pathway is at the core of signaling between plants and soil microbes. In this pathway, calcium- calmodulin-dependent protein kinase (CCaMK) plays a crucial role in integrating signals both arbuscular mycorrhizal (AMS) root nodule (RNS). However, molecular mechanism by which CCaMK coordinates AMS RNS largely unknown. Here, we report that gain-of-function (GOF) variants without regulatory domains activate pathways absence symbiotic partners. This activation requires...
Arbuscular mycorrhizal fungus (AMF) species are some of the most widespread symbionts land plants. Our much improved reference genome assembly a model AMF, Rhizophagus irregularis DAOM-181602 (total contigs = 210), facilitated discovery repetitive elements with unusual characteristics. R. has only ten or 11 copies complete 45S rDNAs, whereas general eukaryotic tens to thousands rDNA copies. rDNAs highly heterogeneous and lack tandem repeat structure. These findings provide evidence for...
Arbuscular mycorrhizal symbiosis (AMS) and root nodule (RNS) are mutualistic plant-microbe interactions that confer nutritional benefits to both partners. Leguminous plants possess a common genetic system for intracellular with AM fungi rhizobia. Here we show CERBERUS NSP1, which respectively encode an E3 ubiquitin ligase GRAS transcriptional regulator have previously only been implicated in RNS, involved fungal infection Lotus japonicus. Hyphal elongation along the longitudinal axis of was...
Many leguminous plants have a unique ability to reset and alter the fate of differentiated root cortical cells form new organs nitrogen-fixing nodules during legume-Rhizobium symbiosis. Recent genetic studies on role cytokinin signaling reveal that activation is crucial nodule organogenesis process. However, mechanism underlying initiation poorly understood due low number genes been identified. Here, we identified novel nodulation-deficient mutant named vagrant infection thread 1 (vag1)...
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Nitrogen-fixing rhizobia and arbuscular mycorrhizal fungi (AMF) form symbioses with plant roots these are established by precise regulation of symbiont accommodation within host cells. In model legumes such as Lotus japonicus Medicago truncatula, enter into through an intracellular invasion system that depends on the formation a root-hair infection thread (IT). While IT-mediated is thought to be most evolutionarily derived system, some studies have indicated basal intercellular can replace...
The effects of the Oryza sativa calcium/calmodulin-dependent protein kinase OsCCaMK genotype (dominant homozygous [D], heterozygous [H], recessive [R]) on rice root-associated bacteria, including endophytes and epiphytes, were examined by using a Tos17 mutant line under paddy upland field conditions. Roots sampled at flowering stage subjected to clone library analyses. relative abundance Alphaproteobacteria was noticeably decreased in R plants both conditions (0.8% 3.0%, respectively) those...