A5103078467- Cardiac Ischemia and Reperfusion
- MicroRNA in disease regulation
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Sperm and Testicular Function
- Mitochondrial Function and Pathology
- Air Quality and Health Impacts
- Nitric Oxide and Endothelin Effects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Porphyrin and Phthalocyanine Chemistry
- Electron Spin Resonance Studies
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Histone Deacetylase Inhibitors Research
- Carcinogens and Genotoxicity Assessment
- interferon and immune responses
- Indoor Air Quality and Microbial Exposure
- Bone Metabolism and Diseases
- Circular RNAs in diseases
- Mesoporous Materials and Catalysis
- Cancer-related molecular mechanisms research
- Effects of Radiation Exposure
- Catalytic Processes in Materials Science
Lanzhou University
2013-2024
Cardiovascular Institute of the South
2017
The progression and metastasis of oral squamous cell carcinoma (OSCC) are highly influenced by cancer stem cells (CSCs) due to their unique self-renewal plasticity. In this study, data were obtained from a single-cell RNA-sequencing dataset (GSE172577) in the GEO database, LASSO-Cox regression analysis was performed on 1344 CSCs-related genes establish six-gene prognostic signature (6-GPS) consisting ADM, POLR1D, PTGR1, RPL35A, PGK1, P4HA1. High-risk scores significantly associated with...
Background: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthases (NOSs) for the synthesis (NO). BH4 therapy can reverse disease-related redox disequilibrium observed with deficiency. However, whether exerts a protective effect against radiation-induced damage to cardiomyocytes remains unknown. Methods: Clonogenic assays were performed determine effects X-ray on H9c2 cells or without treatment. The contents lactate dehydrogenase (LDH), superoxide dismutase (SOD), and...
In this study, we assessed the toxic effects of formaldehyde (FA) on mouse bone marrow mesenchymal stem cells (BM-MSCs). Cytotoxicity was measured by using MTT assay. DNA strand breakage detected standard alkaline comet assay and modified with proteinase K (PK). DNA-protein crosslinks (DPCs) were KCl-SDS precipitation We found that FA at a concentration from 75 to 200 μM inhibited cell survival induced DPCs over 125 μM. The PK-modified showed FA-induced increased in dose-dependent manner On...
The present study was designed to elucidate the key parameters associated with X-ray radiation induced oxidative stress and effects of STS on X-ray-induced toxicity in H9c2 cardiomyocytes. Cytotoxicity assessed by MTT. Antioxidant activity evaluated SOD MDA. Apoptosis measured flow cytometry, Hoechst 33258, clonogenic survival assay, western blot. It found that cell viability cells exposed significantly decreased a dose-dependent manner cycle arrest at G0/G1 phase as well apoptosis....
The process of cardiomyocyte swelling involves changes biomechanical properties and profiles cellular genes. Although many genes have been proved to regulate cell edema cardiomyocyte, the mechanisms involved in this event, as well cell, remain unknown.Methods Results:Whether histone deacetylase 1 (HDAC1) inhibition protects against hypoxia-induced H9c2 is examined study. Hypoxia-induced cytoskeletal structure that are relevant were also determined. cells treated under a chemical hypoxia...
Male infertility affects approximately 7% of the male population. In about 40% affected patients, etiology remains unknown. Here, we report cases two infertile brothers who have a uniquely prevalent sperm phenotype with completely amorphous heads. To investigate mechanisms familial teratozoospermia heads, chromatin condensation was assessed by aniline blue staining, western blot, structure assay and atomic force microscopy in both 40 control fertile donors. Our results showed an abnormal...
Hypoxia exposure is responsible for the high incidence of congenital heart defects (CHDs) in high-altitude areas, which nearly 20 times higher than that low-altitude areas. However, genetic factors involved are rarely reported. Sestrin2 ( SESN2 ), a hypoxia stress-inducible gene, protects cardiomyocyte viability under stress; thus, polymorphism may be potential risk factor CHD. We performed an association study polymorphisms with CHD two independent groups Han Chinese population from...
Nano silica spheres are synthesized by the TEOS hydrolysis method. The XRD pattern shows that samples’ strongest peaks significantly broadened. Series samples all single phase. From photos of TEM we can see surfaces have a good dispersion and spherical morphology. Hela cell proliferating activity is assessed MTT assay. cell’s morphology Annexin Hoechst 33342 staining employed to determine apoptosis in cells. Result cytocompatibility becomes better with lower concentrations sample. 24h 48h...