A5079412554- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Biochemical and Structural Characterization
- Corneal Surgery and Treatments
- Microbial Community Ecology and Physiology
- Protein Structure and Dynamics
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Enzyme Structure and Function
- Plant and animal studies
- Retinal Development and Disorders
- Bacterial Genetics and Biotechnology
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Fungal Biology and Applications
- Insect and Pesticide Research
- Cellular transport and secretion
- Fungal and yeast genetics research
- Biocrusts and Microbial Ecology
- Identification and Quantification in Food
- Thallium and Germanium Studies
- Electronic Packaging and Soldering Technologies
- Nephrotoxicity and Medicinal Plants
Science for Life Laboratory
2020-2024
Uppsala University
2011-2024
Beactica (Sweden)
2011-2018
Bruker (Germany)
2014
Charité - Universitätsmedizin Berlin
2012
Universitätsklinikum Tübingen
1955
Abstract Calmodulin (CaM) functions depend on interactions with CaM‐binding proteins, regulated by . Induced structural changes influence the affinity, kinetics, and specificities of interactions. The dynamics CaM neurogranin (Ng) region /calmodulin‐dependent kinase II (CaMKII 290−309 ) have been studied using biophysical methods. These proteins opposite dependencies for binding. Surface plasmon resonance biosensor analysis confirmed that interact very rapidly, moderate affinity ( )....
Lateral diffusion on the neuronal plasma membrane of AMPA-type glutamate receptor (AMPAR) serves an important role in synaptic plasticity. We investigated secreted glycoprotein Noelin1 (Olfactomedin-1 or Pancortin) AMPAR lateral mobility and its dependence extracellular matrix (ECM). found that interacts with high affinity, however, without affecting rise- decay time desensitization properties. co-localizes extra-synaptic AMPARs is expressed at synapses activity-dependent manner....
A novel scaffold inhibiting wild type and drug resistant variants of human immunodeficiency virus 1 reverse transcriptase (HIV-1RT) has been identified in a library consisting 1040 fragments. The fragments were significantly different from already known non-nucleoside inhibitors (NNRTIs), as indicated by Tversky similarity analysis. screening strategy involving SPR biosensor-based interaction analysis enzyme inhibition was used. Primary screening, using short concentration series, followed...
A gram-negative, budding, catalase negative, oxidase positive and non-motile bacterium (MBLW1T) with a complex endomembrane system has been isolated from freshwater lake in southeast Queensland, Australia. Phylogeny based on 16S rRNA gene sequence analysis places the strain within family Planctomycetaceae, related to Zavarzinella formosa (93.3 %), Telmatocola sphagniphila %) Gemmata obscuriglobus (91.9 %). Phenotypic chemotaxonomic demonstrates considerable differences type strains of...
Abstract Bacteria of the Planctomycetes phylum have many unique cellular features, such as extensive membrane invaginations and ability to import macromolecules. These features raise intriguing questions about composition their cell envelopes. In this study, we used microscopy, phylogenomics, proteomics examine evolution envelope proteins in Tuwongella immobilis other members Planctomycetes. Cryo-electron tomography data indicated a distance 45 nm between inner outer membranes T. immobilis....
Abstract The honeybee gut microbiome is thought to be important for bee health, but the role of individual members poorly understood. Here, we present closed genomes and associated mobilomes 102 Apilactobacillus kunkeei isolates obtained from honey crop (foregut) honeybees sampled beehives in Helsingborg south Sweden islands Gotland Åland Baltic Sea. Each beehive contained a unique composition repeated sampling similar two suggests that bacterial community stably maintained across...
The A kinase-anchoring protein AKAP79/150 is a postsynaptic scaffold molecule and key regulator of signaling events. At the postsynapse it coordinates phosphorylation dephosphorylation receptors via anchoring kinases phosphatases near their substrates. Interactions between AKAP79 two Ca(2+) -binding proteins caldendrin calmodulin have been investigated here. Calmodulin known interaction partner that has shown to regulate activity kinase PKC in -dependent manner. Pull-down experiments surface...
This work is related to a comprehensive study on the material dependency for solar cell interconnection by means of epoxy based silver conductive glue (CG) and its effect module reliability. The power degradation samples exceeding DH1000 was studied various ribbon coatings, rear side AlAg pad pastes, amount CG different encapsulation materials. We show that choice materials play significant role in terms electro-chemical corrosion. Cheap as non-coated copper were identified which lead very...
The Planctomycetes bacteria have unique cell architectures with heavily invaginated membranes as confirmed by three-dimensional models reconstructed from FIB-SEM images of Tuwongella immobilis and Gemmata obscuriglobus. subcellular proteome T. was examined differential solubilization followed LC-MS/MS analysis, which identified 1569 proteins in total. Tris-soluble fraction contained mostly cytoplasmic proteins, while inner outer membrane were found the Triton X-100 SDS-soluble fractions,...
The kinetic and mechanistic details of the interaction between caldendrin, calmodulin B-domain AKAP79 were determined using a biosensor-based approach. Caldendrin was found to compete with for binding at AKAP79, indicating overlapping sites. Although affinities similar caldendrin (K(D) = 20 nM) 30 nM), their characteristics different. well described by reversible one-step model, but only detected in presence Ca(2+). interacted higher level complexity, deduced be an induced fit mechanism slow...
Durch Gabe therapeutischer Dosen von Germanin (Bayer 205) wurde im Tierexperiment keine Wirkung auf den Schwund Kongorot aus dem Blut und die Ausscheidung Azorubin ausgeübt. Die Überprüfung der Bindung des Germanins an Serumeiweißkörper mit Hilfe Papierelektrophorese hat gezeigt, daß nur Serumalbumin bindet beim pH Blutes 1–2 Moleküle pro Albuminmolekül fixiert werden. Im Vergleich Untersuchungen anderer Autoren wird in Besprechung betont, kompetitiven Eigenschaften Serum zukommen. Bedeutung...
There is a need for strong and tightly regulated promoters to construct more reliable predictable genetic modules synthetic biology metabolic engineering. For this reason we have previously constructed TetR L promoter library the cyanobacterium Synechocystis PCC 6803. In addition L03 showing wide dynamic range of transcriptional regulation, observed L09 as unique in high leaky gene expression under repressed conditions. present study, attempted identify cause leakage. binding was studied by...
The emergence of new proteins is a central question in biology. Most tertiary protein folds known to date appear have an ancient origin, but it clear from bioinformatic analyses that continuously emerge all organismal groups. However, there paucity experimental data on regarding their structure and biophysical properties. We performed detailed phylogenetic analysis identified 48 putative open reading frames the honeybee-associated bacterium Apilactobacillus kunkeei for which no or few...
Extracellularly released particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their compositions functional roles differ between species also strains of the same species. In this study, we determined composition vesicles protein particles identified in cell-free pellets two Apilactobacillus kunkeei, a defensive symbiont honeybees. The were separated from extracellular using density...
ABSTRACT The emergence of new proteins is a central question in biology. Most tertiary protein folds known to date appear have an ancient origin, but it clear from bioinformatic analyses that continuously emerge all organismal groups. However, there paucity experimental data on regarding their structure and biophysical properties. We performed detailed phylogenetic analysis identified 48 putative open reading frames the honeybee-associated bacterium Apilactobacillus kunkeei for which no...