A5025561641- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Reproductive biology and impacts on aquatic species
- RNA modifications and cancer
- Cancer-related gene regulation
- Seed Germination and Physiology
- Epigenetics and DNA Methylation
- Renal and related cancers
- Animal Nutrition and Physiology
- Aquaculture Nutrition and Growth
- Livestock and Poultry Management
- Mesenchymal stem cell research
- Food and Agricultural Sciences
- Tissue Engineering and Regenerative Medicine
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Comparative Animal Anatomy Studies
- Genetic and phenotypic traits in livestock
- Reproductive Physiology in Livestock
- Exercise and Physiological Responses
- Thermoregulation and physiological responses
- Bee Products Chemical Analysis
- Cancer Cells and Metastasis
- Infrared Thermography in Medicine
Chiang Mai University
2014-2025
Azabu University
2014
University of Veterinary Medicine
2004
For nearly three decades, interspecies somatic cell nuclear transfer (iSCNT) has been explored as a potential tool for cloning, regenerative medicine, and wildlife conservation. However, developmental inefficiencies remain major challenge, largely due to persistent barriers in nucleocytoplasmic transport, mitonuclear communication, epigenome crosstalk. This review synthesized peer-reviewed English articles from PubMed, Web of Science, Scopus, spanning using relevant keywords explore the...
Abstract N6-methyladenosine (m6A) catalyzed by METTL3 regulates the maternal-to-zygotic transition in zebrafish and mice. However, role mechanism of METTL3-mediated m6A methylation blastocyst development remains unclear. Here, we show that sustains porcine via negatively modulating autophagy. We found reduced levels triggered knockdown caused embryonic arrest during morula-blastocyst developmental defects trophectoderm cells. Intriguingly, overexpression early embryos resulted increased...
Sperm sexing technique is favored in the dairy industry. This research focuses on efficiency of bovine sperm using magnetic-activated cell sorting (MACS) by scFv antibody against Y-chromosome-bearing (Y-scFv) coupled to magnetic microbeads and its effects kinematic variables, quality, X/Y-sperm ratio. In this study, optimal concentration Y-scFv coupling surface was 2-4 mg/mL. PY-microbeads revealed significantly enriched (Y-sperm) eluted fraction (78.01-81.43%) X-chromosome-bearing (X-sperm)...
N6-Methyladenosine (m6A) regulates oocyte-to-embryo transition and the reprogramming of somatic cells into induced pluripotent stem cells. However, role m6A methylation in porcine early embryonic development its characteristics cell nuclear transfer (SCNT) embryos are yet to be known. Here, we showed that was essential for normal aberrant SCNT embryos. We identified a persistent occurrence between 1-cell blastocyst stages levels abruptly increased during morula-to-blastocyst transition....
In this study we evaluated the influence of passage number on phenotypic characteristics feline adipose-derived stem cells (ASCs) in order to develop a broader understanding their dynamics. Feline ASCs were individually isolated from five domestic cats and subjected proliferative culture at (P) 2, 6 10. The each sub-passage examined regard properties associated with multipotent mesenchymal (MSCs), such as morphology, proliferation kinetics, self-renewal, expression MSCs-specific surface...
Zygotic genome activation (ZGA) plays an essential role in early embryonic development. Vitrification is a common assisted reproductive technology that frequently reduces the developmental competence of embryos. However, effect vitrification on porcine ZGA and gene expression during remains largely unclear. Here, we found pronuclear zygotes derived from parthenogenetic (PA) vitro fertilization (IVF) resulted significant reduction rates 2-cell, 4-cell, blastocysts, but did not affect quality...
The recent advances in sperm cryopreservation transcend cryobanking and other assisted reproductive technologies. Since its discovery, has contributed positive impacts on animal breeding as well genetic exchange, improvement, conservation efforts. However, cryoinjury variabilities outcomes remain key challenges to cryobiology. present work explored the molecular bases for such freezability differences freezing-thawing injuries ruminant sperm. Relevant biomarkers identified seminal plasma...
Background Elephants are the largest and heaviest living terrestrial animals, but information on their histology is still lacking. This study provides a unique insight into elephant’s organs also comparison between juvenile Asian elephants adult or other species. Here we report histological structure of 24 organs, including skin, brain (cerebrum, cerebellar hemisphere, vermis, thalamus, midbrain), spinal cord, sciatic nerve, striated skeletal muscle, cardiac bone (flat long bone), cartilage...
The objective of this study was to determine the effect albumin and cholesterol substituted for egg yolk in goat semen extender after frozen-thawed on quality pregnancy rate. collected from three bucks by using artificial vagina. divided into 5 groups; group 1, seminal plasma removed 20% added (standard), 2, 2% whole (control), 3, 4 mg/mL semen, 4, 5, extenders were semen. Semen loaded 0.25 mL straw frozen automatic freezing machine stored liquid nitrogen. thawed at 37 OC 30 seconds. It...
This study aimed to investigate gene expression patterns throughout preimplantation embryos and their comparative in morphological quality blastocysts using real-time RT-PCR. Bovine oocytes underwent maturation process, fertilized were cultured vitro. Degree of fragmentation differentiation the criteria categorize day-7 as good poor quality. All from each developmental stage used quantify transcript abundance. It was found that E-cadherin highly expressed immature mature oocytes, then it...
Abstract At fertilization, inositol 1,4,5‐trisphosphate receptor type 1 ( IP 3 R1 ) has a crucial role in Ca 2+ release mammals. Expression levels, localization and phosphorylation of are important for its function, but it still remains unclear which molecule(s) regulates behavior pig oocytes. We examined whether there was difference after vitro or vivo maturation In mouse oocytes, large clusters were formed the cortex oocyte except ring‐shaped band adjacent to spindle. However, no such...
Induction of demyelination in the central nervous system (CNS) experimental mice using cuprizone is widely used as an animal model for studying pathogenesis and treatment demyelination. However, different mouse strains result pathological outcomes. Moreover, because current medicinal treatments are not always effective multiple sclerosis patients, so study exogenous cell transplantation great importance. The aims present were to establish alternative ICR outbred evaluate effects intravenous...
Snakehead fish protein concentrate (SFC) shows promising recovery of excellent content that has broad potential applications, including the future use its albumin as a sperm capacitation promoter. However, quantity snakehead recovered depends on several variables, habitat in which thrives, extraction procedures, and assay employed. To optimise retrieved from flesh with more reliable results, multiple methods were undertaken through preparation analysed using Bromocresol purple (BCP) assay....
Brahman cattle (Bos indicus) are the breed suitable for beef production in tropical climate rearing conditions, where use of sexed semen helps to pass on good genetics superior sire and expand herd rapidly efficiently. This research evaluated motility, kinematic variables, sex ratio sperm post-thaw using magnetic-activated cell sorting conjugated with Y-scFv antibodies (M-Zlex) by computer-assisted analysis (CASA) imaging flow cytometry. Fresh samples from a total 26 ejaculates were...
This study investigated the effects of gonadotropin-releasing hormone (GnRH) vaccine on pig growth, carcass quality, and sensory perceptions pork meat by Thai consumers. Male crossbred pigs (n = 30) were separated into three groups 10 each: boars, immunocastrated pigs, surgically castrated pigs. The group was immunized at 13 19 weeks age with GnRH (400 μg/dose). All slaughtered 24 age. Blood samples collected testes size determined. Testes weight back fat thickness recorded time slaughter,...
The development of assisted reproductive technologies (ART) in mammalian species such as vitro embryo production (IVEP) has the potential to provide great benefits for significant population increase, improve genetic performance and advancement, reduce transmission venereal diseases. Correspondingly, capacitation sperm is also paramount, related ability fertilize oocytes, was created imitate vivo conditions female tract. Amid developments, studies on how far progressed mimicking scenes have...
Pradu-hangdum is a distinctive Thai native pure breed chicken. To conserve the of this chicken species, artificial insemination was invested. The key to highly successful output technique high quality semen. Hence, objective present study characterize Pradu-hangdam sperms and their longevity freezability in BHSV extender. semen 30 randomized male were collected. macroscopic microscopic examination used determine sperm characters. results revealed that contained normal spiral-shaped head,...