A5004821325- Optical Network Technologies
- Asthma and respiratory diseases
- Photonic Crystal and Fiber Optics
- Advanced Photonic Communication Systems
- Advanced Fiber Laser Technologies
- Advanced Fiber Optic Sensors
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Receptor Mechanisms and Signaling
- Advanced Optical Network Technologies
- Immune Response and Inflammation
- Fatty Acid Research and Health
- Sphingolipid Metabolism and Signaling
- Carbohydrate Chemistry and Synthesis
- Photonic and Optical Devices
- Neuropeptides and Animal Physiology
- Respiratory and Cough-Related Research
- Anesthesia and Neurotoxicity Research
- Dermatology and Skin Diseases
- Semiconductor Lasers and Optical Devices
- Intensive Care Unit Cognitive Disorders
- IL-33, ST2, and ILC Pathways
- Chemical Synthesis and Analysis
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Peptides and Activities
Juntendo University
2015-2024
Sumitomo Electric Industries (Japan)
1998-2016
Kyushu University
2008-2015
Sumitomo Electric Industries (United States)
1997-2014
Kumamoto University
2010
The University of Tokyo
2002-2008
National Institute of Advanced Industrial Science and Technology
2006
Japan Science and Technology Agency
2003-2006
Keio University
1996-1997
Tohoku University
1993-1995
G2A (from G2 accumulation) is a G-protein-coupled receptor (GPCR) that regulates the cell cycle, proliferation, oncogenesis, and immunity. shares significant homology with three GPCRs including ovarian cancer GPCR (OGR1/GPR68), GPR4, T death-associated gene 8 (TDAG8). Lysophosphatidylcholine (LPC) sphingosylphosphorylcholine (SPC) were reported as ligands for GPR4 OGR1 (SPC only), glycosphingolipid psychosine was ligand TDAG8. As proton-sensing (Ludwig, M. G., Vanek, M., Guerini, D., Gasser,...
We have developed a fiber-based frequency comb system consisting of simple mode-locked fiber laser and backward pumping amplifier combined with highly nonlinear short zerodispersion wavelength. As result, the signal to noise ratio obtained carrier-envelope-offset beat is larger than 45 dB at bandwidth 100 kHz. Furthermore, we succeeded in measuring optical frequencies 1542-nm acetylene-stabilized 532-nm iodinestabilized Nd:YAG continuously for more one week using system. The long-term...
Activated blood platelets and macrophages metabolize prostaglandin H(2) into thromboxane A(2) 12(S)-hydroxyheptadeca-5Z, 8E, 10E-trienoic acid (12-HHT) in an equimolar ratio through the action of synthase. Although it has been shown that 12-HHT is abundant tissues bodily fluids, this compound long viewed as a by-product lacking any specific function. We show natural ligand for leukotriene B(4) (LTB(4)) receptor-2 (BLT2), G protein-coupled receptor was originally identified low-affinity...
Silica-based highly nonlinear fibers (HNLFs) have been utilized as platforms for various applications, including fiber lasers, optical amplifiers, and signal processings. For the practical nonlinearity enhancement without degrading attenuation tailoring chromatic dispersions remain key issues. Herein, we initially discuss design of HNLFs desired applications. Then fabrication results, with a longitudinally uniform zero-dispersion wavelength or optimized higher order dispersion, are...
Leukotriene B4 (LTB4) receptor type 2 (BLT2) is a G protein–coupled (GPCR) for 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) and LTB4. Despite the well-defined proinflammatory roles of BLT1, in vivo functions BLT2 remain elusive. As mouse highly expressed epidermal keratinocytes, we investigated role 12-HHT/BLT2 axis skin wound healing processes. 12-HHT accumulated fluid mice, BLT2-deficient mice exhibited impaired re-epithelialization delayed closure after punching. Aspirin...
Homoeostatic regulation of the acid-base balance is essential for cellular functional integrity. However, little known about molecular mechanism through which regulates responses. Here, we report that bicarbonate ions activate a G protein-coupled receptor (GPCR), i.e., GPR30, leads to
Silica-based optical fibers are now being used in various applications which utilize nonlinear effects fiber. In addition to enhancing nonlinearity of fibers, tailoring chromatic dispersion is one the important design issues such applications. Highly dispersion-shifted have been developed, and a very compact wavelength converter module has demonstrated by using Dispersion-flattened dispersion-decreasing also proved be highly effective for supercontinuum generation.
The positions of double bonds along the carbon chain methylene interrupted polyunsaturated fatty acids are unique identifiers specific derived from biochemical reactions that occur in cells. It is possible to obtain direct structural information as these bond using tandem mass spectrometry after collisional activation carboxylate anions an acetone adduct at each formed by photochemical Paternò-Büchi reaction with acetone. This can be carried out exposing a small portion inline fused silica...
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used to reduce inflammation by suppressing cyclooxygenases (COXs). NSAID eye drops frequently prescribed after ocular surgery and pain, but this treatment has clinically significant side effects, including corneal ulcer perforation. The molecular mechanisms underlying these effects remain unknown. Recently, the COX product 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) was identified as an endogenous ligand for leukotriene B4...
The glutamate receptor δ2 (GluRδ2) subunit is selectively expressed in cerebellar Purkinje cells and plays an important role long-term depression, motor learning, coordination, synapse development. We identified a novel GluRδ2-interacting protein, named Delphilin, that contains single PDZ domain formin homology (FH) domains FH1 FH2 plus coiled-coil structure. As far as we know, this the first reported protein both FH domains. Yeast two-hybrid surface plasmon resonance (SPR) analyses...
BLT2 is a low-affinity leukotriene B(4) (LTB(4)) receptor that activated by 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) and LTB(4). Despite the well-defined proinflammatory roles of BLT1, in vivo functions remain elusive. To clarify role BLT receptors intestinal inflammation, we assessed susceptibility to dextran sodium sulfate (DSS)-induced colitis mice lacking either BLT1 or BLT2. BLT2(-/-) exhibited increased sensitivity DSS as compared wild-type BLT1(-/-) mice, with more...
12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) has long been considered a by-product of thromboxane A2 (TxA2) biosynthesis with no biological activity. Recently, we reported 12-HHT to be an endogenous ligand for BLT2, low-affinity leukotriene B4 receptor. To delineate the biosynthetic pathway 12-HHT, established method that enables us quantify various eicosanoids and using LC-MS/MS analysis. During blood coagulation, levels increased in time-dependent manner were relatively higher...
Diabetic retinopathy (DR) is a widespread vision-threatening disease, and neuroretinal abnormality should be considered as an important problem. Brain-derived neurotrophic factor (BDNF) has recently been possible treatment to prevent DR-induced damage, but how BDNF upregulated in DR remains unclear. We found increase hydrogen peroxide (H2O2) the vitreous of patients with DR. confirmed that human retinal endothelial cells secreted H2O2 by high glucose, reduced cell viability MIO-M1, Müller...
It has been made clear through model simulations that dispersion-flattened and decreasing fiber (DFDF) is one of the most suitable fibers for broad-band supercontinuum (SC) generation. SC generation In a bandwidth 280 nm with intensity fluctuation less than 15 dB experimentally observed by using DFDF designed manufactured an optimized dispersion profile.
Leukotriene B4 (LTB4) has been implicated in the pathogenesis of allergic diseases. BLT2, a low-affinity LTB4 receptor, is activated by and 12(S)-hydroxyheptadeca-5Z,8E,10E -trienoic acid (12-HHT). Although high-affinity receptor BLT1 shown to exert proinflammatory roles, role BLT2 inflammation not clarified. To study function development asthma, we used mice model ovalbumin (OVA)-induced airway disease. The 12-HHT levels were elevated bronchoalveolar lavage (BAL) fluids...
GPCRs are involved in numerous physiologic functions and important drug targets. Although the epithelial barrier is for protection from invading pathogens, correlation between function remains unknown. Leukotriene B4 (LTB4) receptor type 2 (BLT2), mainly expressed cells, a GPCR 12(S)-hydroxyheptadeca-5Z,8E,10E-trienoic acid (12-HHT) LTB4. In our study, BLT2 localized at lateral membrane BLT2-overexpressing Madin-Darby canine kidney (MDCK) II cells small intestine of BLT2-transgenic mice....
Sepsis continues to result in high morbidity and mortality. General anesthesia is often administered septic patients, but the impacts of general on host defense are not well understood. can be given by volatile intravenous anesthetics. Our previous vitro study showed that anesthetic isoflurane directly inhibits leukocyte function-associated antigen-1 (LFA-1) macrophage-1 antigen (Mac-1), critical adhesion molecules leukocytes. Thus, role exposure vivo LFA-1 Mac-1 function was examined using...
Age-related macular degeneration (AMD), a progressive chronic disease of the central retina, is associated with aging and leading cause blindness worldwide. Here, we demonstrate that leukotriene B4 (LTB4) receptor 1 (BLT1) promotes laser-induced choroidal neovascularization (CNV) in mouse model for wet-type AMD. CNV was significantly less BLT1-deficient (BLT1-KO) mice compared BLT1-WT controls. Expression several proangiogenic profibrotic factors lower BLT1-KO eyes than eyes. LTB4 production...
General anesthesia has been the requisite component of surgical procedures for over 150 yr. Although immunomodulatory effects volatile anesthetics have growingly appreciated, molecular mechanism not understood. In septic mice, commonly used anesthetic isoflurane attenuated production 5-lipoxygenase products and IL-10 reduced CD11b intercellular adhesion molecule-1 expression on neutrophils, suggesting attenuation TLR4 signaling. We confirmed signaling in vitro their direct binding to...
Leukotriene B4 (LTB4) receptor 1 (BLT1), a high-affinity GPCR for LTB4, plays important roles in acute and chronic inflammatory diseases. Although the LTB4-BLT1 axis is known to promote inflammation, no studies have defined binding proteins that modulate signaling. In this study, advanced glycation end products (RAGE) interacted with BLT1 human cervical epithelial HeLa cells. RAGE increased LTB4-BLT1-dependent ERK phosphorylation inhibited activation of NF-κB up-regulation proinflammatory...