A5102798720- Microbial Natural Products and Biosynthesis
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Ginseng Biological Effects and Applications
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Polysaccharides Composition and Applications
- Silymarin and Mushroom Poisoning
- Enzyme Production and Characterization
- Pharmacological Effects of Natural Compounds
- Mycotoxins in Agriculture and Food
- Microbial Metabolism and Applications
- Abdominal Surgery and Complications
- Proteoglycans and glycosaminoglycans research
- Phytochemicals and Antioxidant Activities
- Exercise and Physiological Responses
- Magnesium in Health and Disease
- Biological and pharmacological studies of plants
- Synthesis and bioactivity of alkaloids
- Natural Antidiabetic Agents Studies
- Bioactive natural compounds
- Phytochemical Studies and Bioactivities
- Enhanced Recovery After Surgery
Wuhan University
2018-2025
Shanghai Jiao Tong University
2021-2025
Shanghai Advanced Research Institute
2025
Shandong University of Traditional Chinese Medicine
2017-2024
Shandong Shierming Eye Hospital
2024
Xuzhou Medical College
2018-2019
Affiliated Hospital of Xuzhou Medical College
2019
Wenzhou Hospital of Traditional Chinese Medicine
2017
Stanford University
2010
Abstract Aspergillus oryzae is a widely used host for heterologous expression of fungal natural products. However, the vectors previously developed are not convenient use and screening positive transformants by PCR fermentation time- effort-consuming. Hence, three plug-and-play were here multi-gene liquid chromatography mass spectrometry detection was introduced to screen transformants. Using rug BGC verification, we demonstrated that perform well feasible For deleterious gene expression, P...
Controlling the incorporation of extender units is a key strategy for manipulating polyketide scaffolds. Current understanding suggests that unit by modular synthase (PKS) primarily regulated acyltransferase (AT) domains, along with ketosynthase (KS), ketoreductase (KR) and thioesterase (TE) domains. In this study, we investigated mechanism underlying specific benzyl side chain at C7 position UK‐2A, both in vivo vitro. Our findings reveal benzylmalonyl‐CoA governed inter‐domain coordination...
We report the first biosynthesis of trifluoromethyl (TFM)-containing extender unit and its incorporation into polyketide biosynthetic pathway. Using engineered enzymes UkaQFAV Arm13-ACCase, we synthesized a TFM-containing unit, which was successfully integrated antimycin structure. This modification resulted in stereochemical changes, leading to formation an unusual 7S-epimer. The TFM-modified derivatives exhibited significantly enhanced antifungal activity, providing new strategy for...
Abstract Polyketides are a large family of pharmaceutically important natural products, and the structural modification their scaffolds is significant for drug development. Herein, we report high‐resolution X‐ray crystal structures broadly selective acyltransferase (AT) from splenocin polyketide synthase (SpnD‐AT) in apo form complex with benzylmalonyl pentynylmalonyl extender unit mimics. These revealed molecular basis stereoselectivity substrate specificity SpnD‐AT, enabled engineering...
The purpose of this study is to develop a robust approach obtain β glucans from Lentinus edodes and characterize their structural biological properties for sustainable utilization. alkali extraction was optimized with an orthogonal experimental design, concise process obtaining specific targeting polysaccharides developed in study. After purification Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, methylation analysis, NMR spectroscopy were employed...
Colorectal cancer is one of the leading causes tumor-associated death, and traditional Chinese medicine (TCM) classifies colorectal into various subtypes mainly according to symptomatic pattern identification (ZHENG). Here, we investigated difference in metabolic profiles serum by comparing subjects with Nondeficiency (ND), Qi deficiency (QD), Yin (YD). The ratio carcinoembryonic antigen (CEA) was higher YD pattern, carbohydrate 19-9 (CA19-9) both QD, compared ND. As a result metabolomics...
The infusion of magnesium sulfate is well known to reduce arterial pressure and attenuate hemodynamic response pneumoperitoneum. This study aimed investigate whether different doses can effectively the pneumoperitoneum-related changes release vasopressin in patients undergoing laparoscopic gastrointestinal surgery.Sixty-nine partial gastrectomy were randomized into three groups: group L received 30 mg/kg loading dose 15 mg/kg/h continuous maintenance for 1 h; H 50 followed by S (control...
Abstract β‐Ketoacyl‐ACP synthase III (KAS III) is a class of important C−C bond‐forming enzymes that mostly catalyze the initiation polyketide and fatty acid biosynthesis. In this study, we elucidated an unusual (PKS) system involves two unique KAS IIIs (AsuC3 C4) in biosynthesis upper triene chain asukamycin. Significantly, AsuC3 C4 have both iterative elongation activity, while being functionally biased toward steps, respectively. Mutational analysis revealed their catalytic activities...
Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied optimize medium components endophytic fungus. Results indicated that endophyte Aspergillus terreus had matrine. The single factor tests demonstrated potato starch best carbon source and combination peptone NH4NO3 optimal nitrogen A. terreus. model RSM predicted gain maximal production at 20.67 μg/L, when 160.68 g/L,...
A cryptic tetronate biosynthetic pathway was identified in Kitasatospora niigatensis DSM 44781 via heterologous expression. Distinct from the currently known pathways, this system utilizes a partially functional nonribosomal peptide synthetase and broadly selective polyketide synthase to direct assembly lactonization of scaffold. By employing permissive crotonyl-CoA reductase/carboxylase provide different extender units, seven new tetronates (kitaniitetronins A–G) were obtained...
This review presents the chemical constituents and pharmacological actions of Silybum marianum. These include flavonolignans, fatty acids, phenolics other constituents. Furthermore, flavonolignans silymarin isosilychristin, silychristin, silydianin, silybin A, B, isosilybin etc. Pharmacological a well curative effect on non-alcoholic steatohepatitis, UV damage, varieties cancers, diabetes. In addition, its anti-inflammatory, anti-depression more actions. paper will enable marianum lay...
Acyl-CoAs are key precursors of primary and secondary metabolism. Their efficient biosynthesis is often impeded by the limited substrate specificity low in vivo activity acyl-CoA synthetases (ACSs) due to regulatory acylation catalytically important lysine residue motif A10 (Lys-A10). In this study, we identified an unusual ACS (UkaQ) from UK-2A biosynthetic pathway that naturally lacks Lys-A10 exhibits extraordinarily broad specificity. Protein engineering significantly improved its...