A5081401471- Alkaline Phosphatase Research Studies
- Influenza Virus Research Studies
- Pancreatitis Pathology and Treatment
- Vitamin C and Antioxidants Research
- Muscle and Compartmental Disorders
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Marine Biology and Environmental Chemistry
- Bone and Joint Diseases
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Medical and Biological Ozone Research
- Cellular transport and secretion
- Viral Infectious Diseases and Gene Expression in Insects
- Organometallic Compounds Synthesis and Characterization
- Pharmacogenetics and Drug Metabolism
- Escherichia coli research studies
- Lysosomal Storage Disorders Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Animal Disease Management and Epidemiology
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
Nanyang Technological University
2016-2024
Alloksys (Netherlands)
2012-2024
Utrecht University
2005-2023
Wageningen University & Research
2020-2023
AM–Pharma (Netherlands)
2003-2005
Solvay (Netherlands)
1993-2001
Solvay (Belgium)
1999
Alma College
1998
The Netherlands Cancer Institute
1986-1990
La Trobe University
1986
It has been demonstrated that human placental alkaline phosphatase (HPLAP) attenuates the lipopolysaccharide (LPS)-mediated inflammatory response, likely through dephosphorylation of lipid A moiety LPS. In this study, it is also derived from calf intestine (CIAP) able to detoxify mice administered CIAP, 80% animals survived a lethal <i>Escherichia coli</i> infection. piglets, previous LPS detoxification, pharmacokinetic behavior CIAP was studied. clearance shown be dose-independent and...
Hepatitis B surface antigen (HBsAg) particles are secreted by Chinese hamster ovary cells that stably transfected with the S gene of hepatitis virus. The assembly HBsAg into cylindrical and spherical occurred intracellularly within endoplasmic reticulum. accumulated large dilated areas reticulum remained these structures for most time prior to secretion from cells. Once were formed, polypeptides did not appear become associated subsequent intracellular organelle membranes or plasma membrane....
Mixed monolayers containing vesicular stomatitis virus-infected Chinese hamster ovary clone 15B cells (lacking UDP-N-acetylglucosamine transferase I, a Golgi enzyme) and uninfected wild-type were formed. Extensive cell fusion occurs after the monolayer is exposed to pH of 5.0. The virus encoded membrane glycoprotein (G protein) resident in rough endoplasmic reticulum (labeled with [35S]methionine) or complex [3H]palmitate) at time can reach complexes from fusion; G protein present plasma...
A tissue culture method using MDCK cells grown under serum-free conditions was developed to produce an inactivated influenza subunit vaccine. The first clinical data suggest it be equal the conventional egg-derived In a double-blind controlled trial, 2 groups (n = 57 each) of adult volunteers were immunized with experimental bivalent vaccine derived from either or hens' eggs. Each contained 15 μg hemagglutinin A/Taiwan/1/86 (H1N1) and B/Panama/ 45/90. No clinically relevant adverse reactions...
Objective: Alkaline phosphatase may decrease the harmful effects of lipopolysaccharide by detoxifying lipid A. The aim this study was to investigate whether administration alkaline is beneficial in a clinically relevant septic shock model. Design: Interventional laboratory study. Setting: University hospital animal research laboratory. Subjects: Fourteen fasted, anesthetized, invasively monitored, mechanically ventilated, female sheep (27.6 ± 3.9 kg). Interventions: Each received 1.5 g/kg...
ABSTRACT Lipopolysaccharide (LPS) contributes importantly to morbidity and mortality in sepsis. Bovine intestinal alkaline phosphatase (BIAP) was demonstrated detoxify LPS through dephosphorylation. injection combined with BIAP reduced inflammation improved survival various experimental settings. In this study, single-dose intravenous administration of (0.15 IU/g) applied a murine cecal ligation puncture (CLP) model polymicrobial Saline given as control (S group). Treatment prior CLP...
We have used a monoclonal antibody specific for hydrocarbon-induced cytochrome P450 to localize, by electron microscopy, the epitope-specific P450. The was found in rough and smooth endoplasmic reticulum (ER) nuclear envelope of hepatocytes. Significant quantities were not Golgi stacks. also could find any evidence Golgi-associated processing Asn-linked oligosaccharide chains two well-characterized ER membrane glycoprotein enzymes (glucosidase II hexose-6-phosphate dehydrogenase), or...
Alkaline phosphatase (AP) is an enzyme that exhibits anti-inflammatory effects by dephosphorylating inflammation triggering moieties (ITMs). AP administration aims to prevent and treat peri- post-surgical ischemia reperfusion injury in cardiothoracic surgery patients. Recent studies reported intravenous bolus continuous infusion of patients undergoing coronary artery bypass grafting with cardiac valve induce a subsequent release liver-type tissue non-specific alkaline (TNAP) into the...
Abstract Background Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which can neutralize endotoxin as well adenosine triphosphate (ATP), an endogenous danger signal released during brain injury. In this study we assessed potential therapeutic role for AP in inhibiting neuroinflammation using three complementary approaches. Methods Mice were immunized to induce experimental autoimmune encephalomyelitis (EAE) and treated with seven days different phases of disease. addition,...
Introduction Laboratory and clinical data have implicated endotoxin as an important factor in the inflammatory response to cardiopulmonary bypass. We assessed effects of administration bovine intestinal alkaline phosphatase (bIAP), detoxifier, on levels patients undergoing coronary artery bypass grafting. Methods A total 63 grafting were enrolled prospectively randomized. Bovine (n=32) or placebo (n=31) was administered intravenous bolus followed by continuous infusion for 36 hours. The...