A5101462848- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Cancer Genomics and Diagnostics
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Polyamine Metabolism and Applications
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Heat shock proteins research
- Amino Acid Enzymes and Metabolism
- Cannabis and Cannabinoid Research
- Galectins and Cancer Biology
- Genomics and Rare Diseases
- Photoreceptor and optogenetics research
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Retinal Development and Disorders
- Selenium in Biological Systems
- Cell death mechanisms and regulation
- Neuroscience and Neural Engineering
- Lipid metabolism and biosynthesis
- Genetics, Aging, and Longevity in Model Organisms
Hunan Normal University
2019-2024
Southwest Medical University
2024
Northwestern Polytechnical University
2020-2024
Air Force Medical University
2011-2024
Tongji University
2014-2023
Tongji Hospital
2023
Beijing Shijitan Hospital
2023
Binzhou Medical University
2017-2018
Binzhou University
2017-2018
Yangzhou University
2015
BackgroundThe dysregulation of oncogenes and tumor suppressor genes plays an important role in many cancers, including hepatocellular carcinoma (HCC), which is one the most common cancers world. In a previous microarray experiment, we found that DLGAP5 overexpressed HCCs. However, whether up-regulation contributes to hepatocarcinogenesis remains unclear. Methodology/Principal FindingsIn this study, showed was significantly up-regulated 76.4% (168 220) analyzed HCC specimens when compared...
Mitochondrial DNA (mtDNA) mutations are closely implicated in the pathogenesis of multiple cancers, making circulating cell-free mtDNA (ccf-mtDNA) as a potential non-invasive tumor biomarker. However, an effective approach to comprehensively profile ccf-mtDNA is still lacking. In this study, we first characterized by low-depth whole-genome sequencing (WGS) and found that plasma samples exhibited dramatic decrease copy number when compared with fresh tissues. Further analysis revealed had...
To describe the preparation of nano emodin transfersome (NET) and investigate its effect on mRNA expression adipose triglyceride lipase (ATGL) G0/G1 switch gene 2 (G0S2) in tissue diet-induced obese rats. NET was prepared by film-ultrasonic dispersion method. The effects components at different ratios encapsulation efficiency were investigated.The envelopment rate determined ultraviolet spectrophotometry. particle size Zeta potential evaluated Zetasizer analyzer. Sixty male SD rats assigned...
Epithelial ovarian cancer (EOC) heavily relies on oxidative phosphorylation (OXPHOS) and exhibits distinct mitochondrial metabolic reprogramming. Up to now, the evolutionary pattern of somatic DNA (mtDNA) mutations in EOC tissues their potential roles remodelling have not been systematically elucidated.
The polyamines spermidine, spermine, and their precursor putrescine are essential for cell growth the regulation of cycle. Recent studies suggest that excessive accumulation favors either malignant transformation or apoptosis, depending on type stimulus. This study examines involvement in induction apoptosis by DNA topoisomerase I inhibitor, camptothecin. In IEC-6 cells, camptothecin induced within 6 h, accompanied detachment cells. Detached cells showed laddering caspase 3 induction,...
The identification of potential oncogenes plays an important role in finding novel therapeutic targets for many cancers, including hepatocellular carcinoma (HCC), which is one the most common cancers worldwide. In our previous research, using microarray technology, we found that FAM83D was overexpressed HCCs. However, whether overexpression contributes to hepatocarcinogenesis remains unclear. this study, significantly upregulated 76.6% (167 218) HCC specimens at mRNA level and 69.44% (50 72)...
Hepatoblastoma is the most common liver cancer in children, and aggressive subtype often has a poor prognosis lacks effective targeted therapy. Although hepatoblastoma (HB) accompanied by abnormally high expression of transcription factor c-Myc, underlying mechanism remains unclear. In this study, we found that mitochondrial fragmentation was enhanced c-Myc overexpression human HB tissues associated with prognosis. Then, mouse model resembling established via hydrodynamic injection plasmids....
C1297, 2002; 10.1152/ajpcell.00351.2001.We have shown previously that depletion of polyamines delays apoptosis induced by camptothecin in rat intestinal epithelial cells (IEC-6). Mitochondria play an important role the regulation mammalian because apoptotic signals induce mitochondria to release cytochrome c. The latter interacts with Apaf-1 activate caspase-9, which turn activates downstream caspase-3. Bcl-2 family proteins are involved c from mitochondria. In this study, we examined...
Abstract Background Manganese superoxide dismutase (MnSOD) upregulating FoxM1 have previously been demonstrated promoting lung cancer stemness. Isovitexin exhibits antitumor activities in various cancers. This study aimed to assess whether isovitexin inhibits hepatic carcinoma stem-like cells (HCSLCs) features via regulating MnSOD and expression. Methods Second-generation spheres from the cell lines, respectively, were used as HCSLCs. Protein amounts of MnSOD, stemness-associated markers...
Liquid biopsy represents a diagnostic and monitoring tool the circulating cell-free mitochondrial DNA (mtDNA) plays vital role in tumor diagnosis dynamic assessment. Colorectal cancer (CRC) is one of most common fatal cancers worldwide. Mitochondrially encoded NADH dehydrogenase subunit 1 (MT-ND1) encodes biggest respiratory complex I mtDNA, mutations MT-ND1 are CRC. We sought to determine if could be potential biomarker for colorectal cancer. In this study, twenty-two CRC patients at...
Abstract Background CSLCs(Cancer stem cell-like cells), which are central to tumorigenesis, intrinsically influenced by epigenetic modifications. This study aimed elucidate the underlying mechanism involving DNMT1/miR-152-3p/SOS1 axis in regulating self-renewal and tumor growth of LCSLCs (lung cancer stem-like cells). Materials methods Target genes miR-152-3p were predicted using TargetScan Human 8.0. Self-renewal LCSLC compared suspension-cultured non-small cell lung (NSCLC) lines H460 A549...
Ornithine decarboxylase (ODC) is feedback regulated by polyamines. ODC antizyme mediates this process forming a complex with and enhancing its degradation. It has been reported that polyamines induce inhibit activity. Since exogenous can be converted to each other after they are taken up into cells, we used an inhibitor of S-adenosylmethionine decarboxylase, diethylglyoxal bis(guanylhydrazone) (DEGBG), block the synthesis spermidine spermine from putrescine investigated specific roles...
Yiqifumai is a traditional Chinese medicine compound preparation used for the treatment of various vascular diseases in China. However, little known regarding its role microcirculation. The present study investigated effect pretreatment yiqifumai on rat mesentery microcirculatory disturbance induced by LPS. Male Wistar rats were continuously infused with LPS (5 mg kg(-1) h(-1)). parameters evaluated included diameter and red blood cell velocity venules, leukocyte adhesion to venular wall,...
Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions transcription factor or transcriptional co‑activator in the regulation of cellular responses, and may be involved tumor progression metastasis. Data from our previous gene expression profile human microvascular endothelial cells during capillary morphogenesis showed significant alteration IER2 expression, suggesting participate cell angiogenesis. The aim present study was to...
Mitochondrial DNA (mtDNA) mutations alter mitochondrial function in oxidative metabolism and play an important role tumorigenesis. A series of studies have demonstrated that the mtDNA control region (mtCTR), which is essential for replication transcription, represents a mutational hotspot human tumors. However, comprehensive pan-cancer evolutionary pattern analysis mtCTR urgently needed.We generated combined dataset containing 10026 somatic from 4664 patients, covering 20 tumor types based...
Human liver cancer stem-like cells (HLCSLCs) are widely acknowledged as significant factors in the recurrence and eradication of hepatocellular carcinoma (HCC). The sustenance HLCSLCs' stemness is hypothesized to be intricately linked epigenetic process DNA methylation modification genes associated with anticancer properties. present study aimed elucidate stemness-maintaining mechanism HLCSLCs provide a novel idea for clearance HLCSLCs.
Abstract Background Many studies have demonstrated the high efficacy of cell-free nuclear DNA in cancer diagnostics. Compared to DNA, mitochondrial (mtDNA) exhibits distinct characteristics, including multiple copies per cell and higher mutation frequency. However, potential applicability mtDNA (cf-mtDNA) plasma urine remains poorly investigated. Methods Here, we comprehensively analyzed fragmentomic mutational characteristics cf-mtDNA samples from controls patients using next-generation...
The migration of IEC-6 cells is inhibited when the are depleted polyamines by inhibiting ornithine decarboxylase with alpha-difluoromethylornithine (DFMO). Exogenous putrescine, spermidine, and spermine completely restore cell DFMO. Because interconverted during their synthesis catabolism, specific role individual in intestinal migration, as well growth, remains unclear. In this study, we used an inhibitor S-adenosylmethionine decarboxylase, diethylglyoxal bis(guanylhydrazone)(DEGBG), to...
We have previously reported that 8-bromo-7-methoxychrysin (BrMC), a novel synthetic derivative of chrysin, was demonstrated anti-tumor activities against several human cancers, including lung cancer.Interaction between inflammation and cancer stem cell are recently increasingly recognized in tumorigenesis progression.The purpose this study to investigate whether BrMC inhibits stemness H460 cells induced by inflammatory factors (TGF-β combined with TNF-α) its potential mechanism.Our results...
Background. Manganese superoxide dismutase (MnSOD) has been reported to promote stemness of lung cancer stem-like cells (LCSLCs) which had higher glycolytic rates compared with non-CSLCs. Isovitexin exhibited an inhibitory effect on the hepatocellular carcinoma cells. However, whether isovitexin could inhibit promotion LCSLCs mediated by MnSOD through glycolysis remains unclear. Objective. Our study was aimed at investigating inhibits signaling blockage and suppression. Methods. Sphere...
Abstract Ovarian cancer (OC) is the most lethal gynecologic tumor and characterized by a high rate of metastasis. Challenges in accurately delineating metastatic pattern have greatly restricted improvement treatment OC patients. An increasing number studies leveraged mitochondrial DNA (mtDNA) mutations as efficient lineage-tracing markers clonality. We applied multiregional sampling high-depth mtDNA sequencing to determine patterns advanced-stage Somatic were profiled from total 195 primary...
IntroductionMitochondrial fission process 1 (MTFP1) is an inner mitochondrial membrane (IMM) protein implicated in the development and progression of various tumors, particularly lung squamous cell carcinoma (LUSC). This study aims to provide a more theoretical basis for treatment LUSC.MethodsThrough bioinformatics analysis, MTFP1 was identified as novel target gene HIF1A. expression LUSC examined using The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Proteomics Data Commons...