A5061243273- Pancreatic function and diabetes
- Diabetes Management and Research
- Diet, Metabolism, and Disease
- Natural Antidiabetic Agents Studies
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- Nanoparticle-Based Drug Delivery
- Metabolism, Diabetes, and Cancer
- Drug Solubulity and Delivery Systems
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Radiation Therapy and Dosimetry
- Diabetes Treatment and Management
- Phytochemicals and Antioxidant Activities
- Advancements in Transdermal Drug Delivery
- Advanced Drug Delivery Systems
- Retinal Development and Disorders
- RNA Research and Splicing
- Dendrimers and Hyperbranched Polymers
- Liver physiology and pathology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Flavonoids in Medical Research
- Nanoplatforms for cancer theranostics
- Caveolin-1 and cellular processes
Université de Strasbourg
2010-2025
Institut de Cancérologie Strasbourg
2021-2025
Diabète et thérapie cellulaire
2015
Inserm
2010
Centre National de la Recherche Scientifique
2010
Abstract Radiotherapy delivered using photons induces an immune response that leads to modulation of the tumor microenvironment. Clinical studies are ongoing evaluate checkpoint inhibitors in association with photon radiotherapy. At present, there is no publication on radio-induced after proton therapy. Balb/c mice bearing subcutaneous CT26 colon tumors were irradiated by a single fraction 16.4 Gy beam extracted from TR24 cyclotron. RNA sequencing analysis was assessed at 3 days...
Ionizing radiation can influence the antitumor immune response, either activating or suppressing system depending on tumor type and radiotherapy modality. While photon (RT) combined with immunotherapy (IT) is widely studied in clinical trials, proton (PT) IT has not been thoroughly investigated preclinical studies despite its radiobiological advantages. This study aims to explore effects of a hypofractionated PT scheme compared RT efficacy anti-PD-L1 immunotherapy. Balb/c mice bearing...
Transplantation of encapsulated islets in a bioartificial pancreas is promising alternative to free islet cell therapy avoid immunosuppressive regimens. However, hypoxia, which can induce rapid loss islets, major limiting factor. The efficiency oxygen delivery an vitro model involving hypoxia and confined conditions has never been investigated. Oxygen carriers such as perfluorocarbons hemoglobin might improve oxygenation. To verify this hypothesis, study aimed identify the best candidate...
Lateral interactions at the first retinal synapse have been initially proposed to involve GABA by transporter-mediated release from horizontal cells, onto GABA(A) receptors expressed on cone photoreceptor terminals and/or bipolar cell dendrites. However, in mammalian retina, cells do not seem contain systematically or express membrane transporters. We here report that mouse GAD65 GAD67 mRNA, and were weakly but consistently immunostained for GAD65/67. While was readily detected after...
In bioartificial pancreases (BP), the number of islets needed to restore normoglycaemia in diabetic patient is critical. However, confinement a high quantity limited space may impact islet survival, particularly regard low oxygen partial pressure (PO2) such environments. The aim present study was evaluate confined under hypoxia on cell survival. Rat were seeded at three different concentrations (150, 300, and 600 Islet Equivalents (IEQ)/cm(2)) cultured normal atmospheric (160 mmHg) as well...
Background and Purpose Liraglutide improves the metabolic control of diabetic animals after islet transplantation. However, mechanisms underlying this effect remain unknown. The objective study was to evaluate anti‐inflammatory anti‐oxidative properties liraglutide on rat pancreatic islets in vitro vivo . Experimental Approach In , were incubated with 10 μmol·L −1 for 12 24 h. Islet viability functionality assessed. evaluated by measuring CCL2, IL‐6 IL‐10 secretion macrophage chemotaxis....
This study investigated the angiogenic properties of liraglutide in vitro and vivo mechanisms involved, with a focus on Hypoxia Inducible Factor-1α (HIF-1α) mammalian target rapamycin (mTOR).
Islet transplantation is one of the most efficient cell therapies used in clinics and could treat a large proportion patients with diabetes. However, it limited by high requirement pancreas necessary to provide sufficient surviving islet mass hepatic tissue restore normoglycaemia. Reduction organ procurement requirements be achieved extrahepatic using biomaterial that enhances survival function. We report plasma-supplemented hydroxypropyl methylcellulose (HPMC) hydrogel, engineered...
Oxidative stress (OS) plays an important role in type 2 diabetes (T2D) pathogenesis and its complications. New therapies target natural antioxidants as alternative and/or supplemental strategy to prevent control them. Our previous chemical biological studies highlighted the antioxidant activities of cherries, among other fruits vegetables, thus we aimed determine vivo effects 2-month long cherry consumption using a high-fat/high-fructose (HFHF) model diabetic-rats (Lozano et al. Nutr Metab...
Exogenous insulin is the only treatment available for type 1 diabetic patients and mostly administered by subcutaneous (SC) injection in a basal bolus scheme using pens (injection) or pumps (preimplanted SC catheter). Some divergence exists between these two modes of administration, since provide better glycaemic control compared to injections humans. The aim this study was compare impacts administration (single long-acting pump delivery rapid-acting insulin) at same dosage (4 IU/200 g/day)...
Aims . Oxidative stress (OS) plays a major role in type 2 diabetes and its vascular hepatic complications, novel therapeutic approaches include natural antioxidants. Our previous chemical biological studies demonstrated the antioxidant activities of red cabbage (RC), here, we aimed to determine vivo effects 2‐month long RC consumption using high‐fat/high‐fructose model diabetic rats. Results This vegetable, associated with lifestyle measurement, was shown decrease OS increase endothelial NO...
Pancreatic ductal adenocarcinoma is a devastating disease with 5-year overall survival of 9% for all stages. Gemcitabine-based chemoradiotherapy locally advanced pancreatic cancer highly toxic. We conducted an in vitro study to determine whether poly(ADP-ribose) polymerase-1 inhibition radiosensitized gemcitabine-based chemotherapy. Human cell lines, MIA PaCa-2, AsPC-1, BxPC-3 and PANC-1 were treated gemcitabine (10 nM) and/or olaparib (1 µM). Low-LET gamma single dose 2, 5 10 Gy radiations...
The EGFR-targeting antibody cetuximab (CTX) combined with radiotherapy is the only targeted therapy that has been proven effective for treatment of locally advanced head and neck squamous cell carcinoma (LA-HNSCC). Recurrence arises in 50% patients HNSCC years following treatment. In clinicopathological practice, it difficult to assign classes risk because no reliable biomarkers are available predict outcome HPV-unrelated HNSCC. present study, we investigated role Caveolin-1 (Cav1)...
Following the tremendous development of hydrogels for cell therapy, there is now a growing need surgical techniques to validate in vivo scaffold benefits islet transplantation. Therefore, we propose newly designed procedure involving injection hydrogel-embedded pancreatic islets omentum, which considered favorable environment survival and function. Our technique, called h-Omental Matrix Islet filliNG (hOMING) was test hydrogel on function vivo. Islets were implanted omentum diabetic rats...
Regenerative medicine based on cell therapy represents a new hope for curing disease. Current obstacles include proper in vivo validation of the efficiency therapy. For transfer to recipient body, cells often need be combined with biomaterials, especially hydrogels. However, efficacy such graft requires right environment, hydrogel, and site. The omentum might Based example islet transplantation, we developed hOMING (h-Omental Matrix Islet filliNG) technique, which consists injection inside...
Abstract Improving the tumor reoxygenation to sensitize radiation therapy is a cornerstone in oncology. Here, pre‐clinical development of clinically transferable liposomal formulation encapsulating trans sodium crocetinate (NP TSC) reported improve oxygen diffusion through environment. Early pharmacokinetic analysis clinical trial this molecule performed on 37 patients orient define optimal fixed dosage use triple‐negative breast cancer model validate therapeutic combination and NP TSC....
Objectives: Oxidative stress plays a major role in the pathophysiology of type 2 diabetes and today new therapeutics target natural antioxidants as an alternative/additional strategy. Our studies, combining chemistry biology, aimed to compare anti-oxidant properties fruits vegetables (F&V) validate vivo effect selected products on complications. Methodology: HPLC-ABTS.+ assay was realized 27 F&V extracts (polar, nonpolar, oil). In vivo, induced by months high-fat-high-fructose (HFHF) dietary...
Regenerative medicine based on cell therapy represents a new hope for curing disease. Current obstacles include proper in vivo validation of the efficiency therapy. For transfer to recipient body, cells often need be combined with biomaterials, especially hydrogels. However, efficacy such graft requires right environment, hydrogel, and site. The omentum might Based example islet transplantation, we developed hOMING (h-Omental Matrix Islet filliNG) technique, which consists injection inside...
Enhancement of Radiation Therapy Effects Liposome-based nanoparticles encapsulating transcrocetin (NP TSC) act as therapeutic radiosensitizer. In article number 2205961, Alexandre Detappe and co-workers perform a study on mouse to confirm their ability reoxygenate triple negative breast cancer tumors for up 48h. This reoxygenation effect leads normalisation the vasculature which enhances radiation therapy effects.
Abstract Diffuse midline (DMG) and brainstem (DIPG) high-grade gliomas (HGG) bearing H3.3K27M driver mutation are aggressive uncurable brain tumors. We evidenced by the past HIF-2a its transcriptional gene, EPAS1, as a marker of resistance when targeting mTor/HIF-1a pathway. To understand HIF-2α potential role in chronically active hypoxic environment DIPG/DMG, we investigate status cohort pediatric HGGs comprising notably mutant tumors their paired vitro derived models. Using concomitantly...